Abstract
Restriction maps of an α-amylase structural gene clone, λDm65, and of four putative α-amylase pseudogene clones are presented. Two α-amylase structural genes, inverted with respect to each other, are contained in λDm65. Subregions of internal DNA sequence homology within λDm65 and of cross-homology between the presumptive pseudogene clones and λDm65 were determined. Subregions of cross-homology between the Drosophila clones and the mouse α-amylase cDNA clone, pMSa104, were also determined. The presence of functional α-amylase structural genes in λDm65 was verified by injection of appropriate subclones into the germinal vesicle of Xenopus oocytes, followed by incubation of the oocytes under conditions that allowed coupled transcription and translation of injected genes to occur. Subclones of the 3.8- and 5.6- kb EcoRI fragments of λDm65 were shown to code for α-amylase isozymes 1 and 3, respectively, of Drosophila melanogaster Canton-S. Both subclones are homologous to RNA of a size sufficient to accommodate the α-amylase-coding information. No RNA species homologous to other subcloned EcoRI fragments of λDm65 was detected.
Full Text
The Full Text of this article is available as a PDF (1.7 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abraham I., Doane W. W. Genetic regulation of tissue-specific expression of amylase structural genes in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4446–4450. doi: 10.1073/pnas.75.9.4446. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crerar M. M., Swain W. F., Pictet R. L., Nikovits W., Rutter W. J. Isolation and characterization of a rat amylase gene family. J Biol Chem. 1983 Jan 25;258(2):1311–1317. [PubMed] [Google Scholar]
- Doane W. W. Quantitation of amylases in Drosophila separated by acrylamide gel electrophoresis. J Exp Zool. 1967 Apr;164(3):363–377. doi: 10.1002/jez.1401640307. [DOI] [PubMed] [Google Scholar]
- Doane W. W., Treat-Clemons L. G., Gemmill R. M., Levy J. N., Hawley S. A., Buchberg A. M., Paigen K. Genetic mechanism for tissue-specific control of alpha-amylase expression in Drosophila melanogaster. Isozymes Curr Top Biol Med Res. 1983;9:63–90. [PubMed] [Google Scholar]
- Meisler M., Strahler J., Wiebauer K., Thomsen K. K. Multiple genes encode mouse pancreatic amylases. Isozymes Curr Top Biol Med Res. 1983;7:39–57. [PubMed] [Google Scholar]