Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1986 Jan;112(1):65–78. doi: 10.1093/genetics/112.1.65

Molecular Genetics of the ROSY-ACE Region of DROSOPHILA MELANOGASTER

Janos Gausz 1,2, Lucinda M C Hall 1,2, Anne Spierer 1,2, Pierre Spierer 1,2
PMCID: PMC1202691  PMID: 17246310

Abstract

Three hundred and fifteen kilobases of DNA from the rosy-Ace region on chromosome 3R of D. melanogaster have previously been cloned and extensively characterized. We describe the isolation of nine new deficiency mutants that break within the 315-kb interval. The position of these breakpoints on the DNA map was determined by in situ and Southern hybridization. Further, we more precisely mapped the breakpoints of several deletions previously analyzed. The results permit us to delimit sequences essential to the known complementation groups in the region within approximately 20 kb in most cases. However, one gene, B16-1, is shown to contain essential sequences that span about 50 kb. Also, we demonstrate by overlapping deficiencies that a 45-kb DNA segment from the region, which includes one known complementation group, allows limited survival when deleted.

Full Text

The Full Text of this article is available as a PDF (1.7 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bender W., Akam M., Karch F., Beachy P. A., Peifer M., Spierer P., Lewis E. B., Hogness D. S. Molecular Genetics of the Bithorax Complex in Drosophila melanogaster. Science. 1983 Jul 1;221(4605):23–29. doi: 10.1126/science.221.4605.23. [DOI] [PubMed] [Google Scholar]
  2. Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
  3. Bossy B., Hall L. M., Spierer P. Genetic activity along 315 kb of the Drosophila chromosome. EMBO J. 1984 Nov;3(11):2537–2541. doi: 10.1002/j.1460-2075.1984.tb02169.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Garber R. L., Kuroiwa A., Gehring W. J. Genomic and cDNA clones of the homeotic locus Antennapedia in Drosophila. EMBO J. 1983;2(11):2027–2036. doi: 10.1002/j.1460-2075.1983.tb01696.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gausz J., Bencze G., Gyurkovics H., Ashburner M., Ish-Horowicz D., Holden J. J. Genetic Characterization of the 87c Region of the Third Chromosome of DROSOPHILA MELANOGASTER. Genetics. 1979 Dec;93(4):917–934. doi: 10.1093/genetics/93.4.917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ising G., Block K. Derivation-dependent distribution of insertion sites for a Drosophila transposon. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):527–544. doi: 10.1101/sqb.1981.045.01.069. [DOI] [PubMed] [Google Scholar]
  7. Kidd S., Lockett T. J., Young M. W. The Notch locus of Drosophila melanogaster. Cell. 1983 Sep;34(2):421–433. doi: 10.1016/0092-8674(83)90376-8. [DOI] [PubMed] [Google Scholar]
  8. Rubin G. M., Spradling A. C. Genetic transformation of Drosophila with transposable element vectors. Science. 1982 Oct 22;218(4570):348–353. doi: 10.1126/science.6289436. [DOI] [PubMed] [Google Scholar]
  9. Scott M. P., Weiner A. J., Hazelrigg T. I., Polisky B. A., Pirrotta V., Scalenghe F., Kaufman T. C. The molecular organization of the Antennapedia locus of Drosophila. Cell. 1983 Dec;35(3 Pt 2):763–776. doi: 10.1016/0092-8674(83)90109-5. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES