Abstract
The effect of allozyme variation at the sn-glycerol-3-phosphate dehydrogenase (Gpdh) locus on variation in the mechanical power output of the flight muscles of Drosophila melanogaster was investigated. The influence of different rearing and flight temperatures and of their interactions with the Gpdh allozymic genotypes (allotypes) on flight ability also were analyzed. Populations from three continents were used, and Gpdh allotypes were generated from crosses between randomly paired isofemale lines made autozygous for each of the two alleles by inbreeding. Measurements made during tethered flight, together with wing morphology, were used to estimate power output using both Weis-Fogh's and Ellington's formulas.—Analyses of variance (ANOVA) indicated significant main effects for both environmental components (rearing and flight temperatures) but for only one of the three genetic components (genetic backgrounds within continent); Gpdh allotypes and populations (continent of origin) were not significant. The interaction between rearing and flight temperature was highly significant, indicating some physiological adaptation. The effect of Gpdh allozymes depended on both rearing and flight temperature and was either significant or marginally so, depending on which set of formulas was used. In either case, the S/S allotype showed a 2–4% greater power output than the F/F allotype at low temperature for both interactions. In addition, the S/S allotype showed significantly greater power output than the F/F allotype among flies raised at 15° and flown at 15°, whereas the reverse was true for flies raised at 30° and flown at 30°. Significant differences among the three allotypes for GPDH activity level were found in general, with S/S having the highest, F/S intermediate and F/F the lowest activity, and an inverse relationship existed between rearing temperature and activity.—The temperature effects on power output are consistent with the geographical and seasonal variation observed at the Gpdh locus in nature. In general, the results show that Gpdh can be considered a minor polygene affecting quantitative variation in the power output during flight and that genotype-by-environment interaction is an important component of that effect.
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Selected References
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- Alahiotis S., Miller S., Berger E. Natural selection at the alpha-GDH locus in Drosophila. Nature. 1977 Sep 8;269(5624):144–145. doi: 10.1038/269144a0. [DOI] [PubMed] [Google Scholar]
- Ashburner M., Lemeunier F. Relationships within the melanogaster species subgroup of the genus Drosophila (Sophophora) i. inversion polymorphisms in Drosophila melanogaster and Drosophila simulans. Proc R Soc Lond B Biol Sci. 1976 Apr 13;193(1111):137–157. doi: 10.1098/rspb.1976.0036. [DOI] [PubMed] [Google Scholar]
- Curtsinger J. W., Laurie-Ahlberg C. C. Genetic Variability of Flight Metabolism in DROSOPHILA MELANOGASTER. I. Characterization of Power Output during Tethered Flight. Genetics. 1981 Jul;98(3):549–564. doi: 10.1093/genetics/98.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson F. M., Schaffer H. E. Isozyme variability in species of the genus Drosophila. VII. Genotype-environment relationships in populations of D. melanogaster from the Eastern United States. Biochem Genet. 1973 Oct;10(2):149–163. doi: 10.1007/BF00485762. [DOI] [PubMed] [Google Scholar]
- Kotarski M. A., Pickert S., Leonard D. A., LaRosa G. J., MacIntyre R. J. The characterization of alpha-glycerophosphate dehydrogenase mutants in Drosophila melanogaster. Genetics. 1983 Oct;105(2):387–407. doi: 10.1093/genetics/105.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laurie-Ahlberg C. C., Bewley G. C. Naturally occurring genetic variation affecting the expression of sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster. Biochem Genet. 1983 Oct;21(9-10):943–961. doi: 10.1007/BF00483952. [DOI] [PubMed] [Google Scholar]
- Laurie-Ahlberg C. C., Maroni G., Bewley G. C., Lucchesi J. C., Weir B. S. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073–1077. doi: 10.1073/pnas.77.2.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Powers D. A., DiMichele L., Place A. R. The use of enzyme kinetics to predict differences in cellular metabolism, developmental rate, and swimming performance between LDH-B genotypes of the fish, fundulus heteroclitus. Isozymes Curr Top Biol Med Res. 1983;10:147–170. [PubMed] [Google Scholar]
- Rubin G. M., Spradling A. C. Genetic transformation of Drosophila with transposable element vectors. Science. 1982 Oct 22;218(4570):348–353. doi: 10.1126/science.6289436. [DOI] [PubMed] [Google Scholar]
- Watt W. B. Adaptation at Specific Loci. II. Demographic and Biochemical Elements in the Maintenance of the Colias Pgi Polymorphism. Genetics. 1983 Apr;103(4):691–724. doi: 10.1093/genetics/103.4.691. [DOI] [PMC free article] [PubMed] [Google Scholar]