Abstract
Genes of the dilute-short ear (d-se) region of mouse chromosome 9 comprise an array of loci important to the normal development of the animal. Over 200 spontaneous, chemically induced and radiation-induced mutations at these loci have been identified, making it one of the most genetically well-characterized regions of the mouse. Molecular analysis of this region has recently become feasible by the identification of a dilute mutation that was induced by integration of an ecotropic murine leukemia virus genome. Several unique sequence cellular DNA probes flanking this provirus have now been identified and used to investigate the organization of wild-type chromosomes and chromosomes with radiation-induced d-se region mutations. As expected, several of these mutations are associated with deletions, and, in general, the molecular and genetic complementation maps of these mutants are concordant. Furthermore, a deletion break-point fusion fragment has been identified and has been used to orient the physical map of the d-se region with respect to the genetic complementation map. These experiments provide important initial steps for analyzing this developmentally important region at the molecular level, as well as for studying in detail how a diverse group of mutagens acts on the mammalian germline.
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Selected References
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- Copeland N. G., Hutchison K. W., Jenkins N. A. Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs. Cell. 1983 Jun;33(2):379–387. doi: 10.1016/0092-8674(83)90419-1. [DOI] [PubMed] [Google Scholar]
- Deol M. S., Green M. C. Snell's waltzer, a new mutation affecting behaviour and the inner ear in the mouse. Genet Res. 1966 Dec;8(3):339–345. doi: 10.1017/s0016672300010193. [DOI] [PubMed] [Google Scholar]
- Gates W H. Linkage of the Factors for Short-Ear and Density in the House Mouse (MUS MUSCULUS). Genetics. 1928 Mar;13(2):170–179. doi: 10.1093/genetics/13.2.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Markert C L, Silvers W K. The Effects of Genotype and Cell Environment on Melanoblast Differentiation in the House Mouse. Genetics. 1956 May;41(3):429–450. doi: 10.1093/genetics/41.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Modolell J., Bender W., Meselson M. Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1678–1682. doi: 10.1073/pnas.80.6.1678. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Popp R. A., Bailiff E. G., Skow L. C., Johnson F. M., Lewis S. E. Analysis of a mouse alpha-globin gene mutation induced by ethylnitrosourea. Genetics. 1983 Sep;105(1):157–167. doi: 10.1093/genetics/105.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUSSELL W. L. X-ray-induced mutations in mice. Cold Spring Harb Symp Quant Biol. 1951;16:327–336. doi: 10.1101/sqb.1951.016.01.024. [DOI] [PubMed] [Google Scholar]
- Russell L. B., Montgomery C. S., Raymer G. D. Analysis of the albino-locus region of the mouse: IV. Characterization of 34 deficiencies. Genetics. 1982 Mar;100(3):427–453. doi: 10.1093/genetics/100.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Russell W. L., Kelly E. M., Hunsicker P. R., Bangham J. W., Maddux S. C., Phipps E. L. Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5818–5819. doi: 10.1073/pnas.76.11.5818. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zachar Z., Bingham P. M. Regulation of white locus expression: the structure of mutant alleles at the white locus of Drosophila melanogaster. Cell. 1982 Sep;30(2):529–541. doi: 10.1016/0092-8674(82)90250-1. [DOI] [PubMed] [Google Scholar]