Abstract
The DNA from the chromosomal region of the Drosophila rosy locus has been examined in 83 rosy mutant strains. Several spontaneous and radiation-induced alleles were associated with insertions and deletions, respectively. The lesions are clustered in a 4-kb region. Some of the alleles identified on the DNA map have been located on the genetic map by fine-structure recombination experiments. The genetic and molecular maps are collinear, and the alignment identifies the DNA location of the rosy control region. A rosy RNA of 4.5 kb has been identified; its 5' end lies in or near the control region.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
- Campuzano S., Carramolino L., Cabrera C. V., Ruíz-Gómez M., Villares R., Boronat A., Modolell J. Molecular genetics of the achaete-scute gene complex of D. melanogaster. Cell. 1985 Feb;40(2):327–338. doi: 10.1016/0092-8674(85)90147-3. [DOI] [PubMed] [Google Scholar]
- Chovnick A., Ballantyne G. H., Holm D. G. Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster. Genetics. 1971 Oct;69(2):179–209. doi: 10.1093/genetics/69.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chovnick A., Gelbart W., McCarron M., Osmond B. Organization of the rosy locus in Drosophila melanogaster: evidence for a control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1976 Oct;84(2):233–255. doi: 10.1093/genetics/84.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chovnick A., McCarron M., Hilliker A., O'Donnell J., Gelbart W., Clark S. Gene organization in Drosophila. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1011–1021. doi: 10.1101/sqb.1978.042.01.101. [DOI] [PubMed] [Google Scholar]
- Clark S. H., Daniels S., Rushlow C. A., Hilliker A. J., Chovnick A. Tissue-specific and pretranslational character of variants of the rosy locus control element in Drosophila melanogaster. Genetics. 1984 Dec;108(4):953–968. doi: 10.1093/genetics/108.4.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark S. H., McCarron M., Love C., Chovnick A. On the identification of the rosy locus DNA in Drosophila melanogaster: intragenic recombination mapping of mutations associated with insertions and deletions. Genetics. 1986 Apr;112(4):755–767. doi: 10.1093/genetics/112.4.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Covington M., Fleenor D., Devlin R. B. Analysis of xanthine dehydrogenase mRNA levels in mutants affecting the expression of the rosy locus. Nucleic Acids Res. 1984 Jun 11;12(11):4559–4573. doi: 10.1093/nar/12.11.4559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craig E. A., Ingolia T. D., Manseau L. J. Expression of Drosophila heat-shock cognate genes during heat shock and development. Dev Biol. 1983 Oct;99(2):418–426. doi: 10.1016/0012-1606(83)90291-9. [DOI] [PubMed] [Google Scholar]
- Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
- Gelbart W., McCarron M., Chovnick A. Extension of the limits of the XDH structural element in Drosophila melanogaster. Genetics. 1976 Oct;84(2):211–232. doi: 10.1093/genetics/84.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green M. M. Genetic instability in Drosophila melanogaster: De novo induction of putative insertion mutations. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3490–3493. doi: 10.1073/pnas.74.8.3490. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hazelrigg T., Kaufman T. C. Revertants of Dominant Mutations Associated with the Antennapedia Gene Complex of DROSOPHILA MELANOGASTER: Cytology and Genetics. Genetics. 1983 Nov;105(3):581–600. doi: 10.1093/genetics/105.3.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hilliker A. J., Chovnick A. Further observations on intragenic recombination in Drosophila melanogaster. Genet Res. 1981 Dec;38(3):281–296. doi: 10.1017/s0016672300020619. [DOI] [PubMed] [Google Scholar]
- Karch F., Weiffenbach B., Peifer M., Bender W., Duncan I., Celniker S., Crosby M., Lewis E. B. The abdominal region of the bithorax complex. Cell. 1985 Nov;43(1):81–96. doi: 10.1016/0092-8674(85)90014-5. [DOI] [PubMed] [Google Scholar]
- Kidd S., Lockett T. J., Young M. W. The Notch locus of Drosophila melanogaster. Cell. 1983 Sep;34(2):421–433. doi: 10.1016/0092-8674(83)90376-8. [DOI] [PubMed] [Google Scholar]
- Kidwell M. G., Kidwell J. F., Sved J. A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977 Aug;86(4):813–833. doi: 10.1093/genetics/86.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCarron M., Gelbart W., Chovnick A. Intracistronic mapping of electrophoretic sites in Drosophila melanogaster: fidelity of information transfer by gene conversion. Genetics. 1974 Feb;76(2):289–299. doi: 10.1093/genetics/76.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCarron M., O'Donnell J., Chovnick A., Bhullar B. S., Hewitt J., Candido E. P. Organization of the rosy locus in Drosophila melanogaster: further evidence in support of a cis-acting control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1979 Feb;91(2):275–293. doi: 10.1093/genetics/91.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Rubin G. M., Kidwell M. G., Bingham P. M. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982 Jul;29(3):987–994. doi: 10.1016/0092-8674(82)90462-7. [DOI] [PubMed] [Google Scholar]
- Rushlow C. A., Bender W., Chovnick A. Studies on the mechanism of heterochromatic position effect at the rosy locus of Drosophila melanogaster. Genetics. 1984 Nov;108(3):603–615. doi: 10.1093/genetics/108.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rushlow C. A., Chovnick A. Heterochromatic position effect at the rosy locus of Drosophila melanogaster: cytological, genetic and biochemical characterization. Genetics. 1984 Nov;108(3):589–602. doi: 10.1093/genetics/108.3.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scott M. P., Weiner A. J., Hazelrigg T. I., Polisky B. A., Pirrotta V., Scalenghe F., Kaufman T. C. The molecular organization of the Antennapedia locus of Drosophila. Cell. 1983 Dec;35(3 Pt 2):763–776. doi: 10.1016/0092-8674(83)90109-5. [DOI] [PubMed] [Google Scholar]