Skip to main content
NCBI home page
Genetics logoLink to Genetics
. 1986 Apr;112(4):785–802. doi: 10.1093/genetics/112.4.785

Lethal Mutations Flanking the 68c Glue Gene Cluster on Chromosome 3 of DROSOPHILA MELANOGASTER

Madeline A Crosby 1, Elliot M Meyerowitz 1
PMCID: PMC1202777  PMID: 3082712

Abstract

We have conducted a genetic analysis of the region flanking the 68C glue gene cluster in Drosophila melanogaster by isolating lethal and semilethal mutations uncovered by deficiencies which span this region. Three different mutagens were used: ethyl methanesulfonate (EMS), ethyl nitrosourea (ENU) and diepoxybutane (DEB). In the region from 68A3 to 68C11, 64 lethal, semilethal, and visible mutations were recovered. These include alleles of 13 new lethal complementation groups, as well as new alleles of rotated, low xanthine dehydrogenase, lethal(3)517 and lethal(3)B76. Six new visible mutations from within this region were recovered on the basis of their reduced viability; all proved to be semiviable alleles of lethal complementation groups. No significant differences were observed in the distributions of lethals recovered using the three different mutagens. Each lethal was mapped on the basis of complementation with overlapping deficiencies; mutations that mapped within the same interval were tested for complementation, and the relative order of the lethal groups within each interval was determined by recombination. The cytological distribution of genes within the 68A3-68C11 region is not uniform: the region from 68A2,3 to 68B1,3 (seven to ten polytene chromosome bands) contains at least 13 lethal complementation groups and the mutation low xanthine dehydrogenase; the adjoining region from 68B1,3 to 68C5,6 (six to nine bands) includes the 68C glue gene cluster, but no known lethal or visible complementation groups; and the interval from 68C5,6 to 68C10,11 (three to five bands) contains at least three lethal complementation groups and the visible mutation rotated. The developmental stage at which lethality is observed was determined for a representative allele from each lethal complementation group.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akam M. E., Roberts D. B., Richards G. P., Ashburner M. Drosophila: the genetics of two major larval proteins. Cell. 1978 Feb;13(2):215–225. doi: 10.1016/0092-8674(78)90190-3. [DOI] [PubMed] [Google Scholar]
  2. Artavanis-Tsakonas S., Muskavitch M. A., Yedvobnick B. Molecular cloning of Notch, a locus affecting neurogenesis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1977–1981. doi: 10.1073/pnas.80.7.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ballantyne G. H., Chovnick A. Gene conversion in higher organisms: non-reciprocal recombination events at the rosy cistron in Drosophila melanogaster. Genet Res. 1971 Apr;17(2):139–149. doi: 10.1017/s0016672300012131. [DOI] [PubMed] [Google Scholar]
  4. Beckendorf S. K., Kafatos F. C. Differentiation in the salivary glands of Drosophila melanogaster: characterization of the glue proteins and their developmental appearance. Cell. 1976 Nov;9(3):365–373. doi: 10.1016/0092-8674(76)90081-7. [DOI] [PubMed] [Google Scholar]
  5. Bender W., Akam M., Karch F., Beachy P. A., Peifer M., Spierer P., Lewis E. B., Hogness D. S. Molecular Genetics of the Bithorax Complex in Drosophila melanogaster. Science. 1983 Jul 1;221(4605):23–29. doi: 10.1126/science.221.4605.23. [DOI] [PubMed] [Google Scholar]
  6. Bishop J. B., Lee W. R. Chromosome breakage in Drosophila melanogaster induced by a monofunctional alkylating agent (EMS). Mutat Res. 1973 Dec;21(6):327–333. [PubMed] [Google Scholar]
  7. Campbell S. D., Hilliker A. J., Phillips J. P. Cytogenetic analysis of the cSOD microregion in Drosophila melanogaster. Genetics. 1986 Feb;112(2):205–215. doi: 10.1093/genetics/112.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crowley T. E., Meyerowitz E. M. Steroid regulation of RNAs transcribed from the Drosophila 68c polytene chromosome puff. Dev Biol. 1984 Mar;102(1):110–121. doi: 10.1016/0012-1606(84)90179-9. [DOI] [PubMed] [Google Scholar]
  9. Guild G. M., Shore E. M. Larval salivary gland secretion proteins in Drosophila. Identification and characterization of the Sgs-5 structural gene. J Mol Biol. 1984 Nov 5;179(3):289–314. doi: 10.1016/0022-2836(84)90067-6. [DOI] [PubMed] [Google Scholar]
  10. Jarry B. P. Genetical and cytological location of the structural parts coding for the first three steps of pyrimidine biosynthesis in Drosophila melanogaster. Mol Gen Genet. 1979 May 4;172(2):199–202. doi: 10.1007/BF00268283. [DOI] [PubMed] [Google Scholar]
  11. Judd B. H., Shen M. W., Kaufman T. C. The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics. 1972 May;71(1):139–156. doi: 10.1093/genetics/71.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Keller E. C., Jr, Glassman E. Phenocopies of the ma-1 and ry mutants of Drosophila melanogaster: inhibition in vivo of xanthine dehydrogenase by 4-hydroxypyrazolo(3,4-d)pyrimidine. Nature. 1965 Oct 9;208(5006):202–203. doi: 10.1038/208202a0. [DOI] [PubMed] [Google Scholar]
  13. Korge G. Chromosome puff activity and protein synthesis in larval salivary glands of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4550–4554. doi: 10.1073/pnas.72.11.4550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kress H., Meyerowitz E. M., Davidson N. High resolution mapping of in situ hybridized biotinylated DNA to surface-spread Drosophila polytene chromosomes. Chromosoma. 1985;93(2):113–122. doi: 10.1007/BF00293158. [DOI] [PubMed] [Google Scholar]
  15. Roberts D. B., Brock H. W., Rudden N. C., Evans-Roberts S. A Genetic and Cytogenetic Analysis of the Region Surrounding the Lsp-1 beta-Gene in DROSOPHILA MELANOGASTER. Genetics. 1985 Jan;109(1):145–156. doi: 10.1093/genetics/109.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Skaer R. J. Interband transcription in Drosophila. J Cell Sci. 1977 Aug;26:251–266. doi: 10.1242/jcs.26.1.251. [DOI] [PubMed] [Google Scholar]
  17. WELSHONS W. J., VON HALLE E. S. Pseudoallelism at the notch locus in drosophila. Genetics. 1962 Jun;47:743–759. doi: 10.1093/genetics/47.6.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wright T. R., Beermann W., Marsh J. L., Bishop C. P., Steward R., Black B. C., Tomsett A. D., Wright E. Y. The genetics of dopa decarboxylase in Drosophila melanogaster. IV. The genetics and cytology of the 37B10-37D1 region. Chromosoma. 1981;83(1):45–58. doi: 10.1007/BF00286015. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES