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. 1986 Jul;113(3):499–515. doi: 10.1093/genetics/113.3.499

Interaction of DNA Polymerase III γ and β Subunits IN VIVO in SALMONELLA TYPHIMURIUM

Joyce Engstrom 1, Annette Wong 1, Russell Maurer 1
PMCID: PMC1202852  PMID: 3015716

Abstract

We show that temperature-sensitive mutations in dnaZ, the gene for the γ subunit of DNA polymerase III holoenzyme, can be suppressed by mutations in the dnaN gene, which encodes the β subunit. These results support a direct physical interaction of these two subunits during polymerase assembly or function. The suppressor phenotype is also sensitive to modulation by the dnaA genotype. Since dnaA is organized in an operon with dnaN, and dnaA is a regulatory gene of this operon, we propose that the dnaA effect on suppression can best be explained by modulation of suppressor dnaN levels.

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Selected References

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