Abstract
A circadian clock mutant of Neurospora crassa, the most distinctive characteristic of which is the complete loss of temperature compensation of its period length, maps to the frq locus where seven other clock mutants have previously been mapped. This mutant, designated frq-9, is recessive to the wild-type allele and to each of the other frq mutants; thus, it differs from the other mutants, which show incomplete dominance to wild type and to each other. Complementation analysis suggests either that the frq locus is a single gene or that frq-9 is a deletion that overlaps adjacent genes. Preliminary efforts at fine structure mapping have indicated that recombination between certain pairs of frq mutations is less than 0.005%, a distance consistent with the locus being a single gene. The recessive nature of frq-9, coupled with complete loss of temperature compensation, suggests that this mutant may represent the null phenotype of the locus and that the frq gene is involved in the temperature compensation mechanism of the clock.—Genetic mapping studies have placed the frq locus on linkage group VIIR, midway between oli (oligomycin resistance) and for (formate auxotrophy), about 2 map units from each, and clearly indicate that frq and oli are separate genes.
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Selected References
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- Brody S., Dieckmann C., Mikolajczyk S. Circadian rhythms in Neurospora crassa: the effects of point mutations on the proteolipid portion of the mitochondrial ATP synthetase. Mol Gen Genet. 1985;200(1):155–161. doi: 10.1007/BF00383329. [DOI] [PubMed] [Google Scholar]
- Case M. E., Giles N. H. Gene order in the qa gene cluster of Neurospora crassa. Mol Gen Genet. 1976 Aug 10;147(1):83–89. doi: 10.1007/BF00337940. [DOI] [PubMed] [Google Scholar]
- Chaleff R. S. The inducible quinate-shikimate catabolic pathway in Neurospora crassa: genetic organization. J Gen Microbiol. 1974 Apr;81(2):337–355. doi: 10.1099/00221287-81-2-337. [DOI] [PubMed] [Google Scholar]
- Feldman J. F., Hoyle M. N. Complementation analysis of linked circadian clock mutants of Neurospora crassa. Genetics. 1976 Jan;82(1):9–17. doi: 10.1093/genetics/82.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gardner G. F., Feldman J. F. Temperature Compensation of Circadian Period Length in Clock Mutants of Neurospora crassa. Plant Physiol. 1981 Dec;68(6):1244–1248. doi: 10.1104/pp.68.6.1244. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gardner G. F., Feldman J. F. The frq locus in Neurospora crassa: a key element in circadian clock organization. Genetics. 1980 Dec;96(4):877–886. doi: 10.1093/genetics/96.4.877. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reddy P., Zehring W. A., Wheeler D. A., Pirrotta V., Hadfield C., Hall J. C., Rosbash M. Molecular analysis of the period locus in Drosophila melanogaster and identification of a transcript involved in biological rhythms. Cell. 1984 Oct;38(3):701–710. doi: 10.1016/0092-8674(84)90265-4. [DOI] [PubMed] [Google Scholar]
- Sargent M. L., Woodward D. O. Genetic determinants of circadian rhythmicity in Neurospora. J Bacteriol. 1969 Feb;97(2):861–866. doi: 10.1128/jb.97.2.861-866.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sebald W., Machleidt W., Wachter E. N,N'-dicyclohexylcarbodiimide binds specifically to a single glutamyl residue of the proteolipid subunit of the mitochondrial adenosinetriphosphatases from Neurospora crassa and Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1980 Feb;77(2):785–789. doi: 10.1073/pnas.77.2.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith R. F., Konopka R. J. Circadian clock phenotypes of chromosome aberrations with a breakpoint at the per locus. Mol Gen Genet. 1981;183(2):243–251. doi: 10.1007/BF00270625. [DOI] [PubMed] [Google Scholar]
- Zehring W. A., Wheeler D. A., Reddy P., Konopka R. J., Kyriacou C. P., Rosbash M., Hall J. C. P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic Drosophila melanogaster. Cell. 1984 Dec;39(2 Pt 1):369–376. doi: 10.1016/0092-8674(84)90015-1. [DOI] [PubMed] [Google Scholar]
- Zimmermann C. R., Orr W. C., Leclerc R. F., Barnard E. C., Timberlake W. E. Molecular cloning and selection of genes regulated in Aspergillus development. Cell. 1980 Oct;21(3):709–715. doi: 10.1016/0092-8674(80)90434-1. [DOI] [PubMed] [Google Scholar]