Abstract
Recessive lethal mutations have been isolated and used to maintain n + 1 aneuploid strains of Dictyostelium discoideum carrying a duplication of part or all of linkage group VII. The recessive lethal mutations, relA351 and relB352, arose spontaneously in diploids; no mutagenic treatment was used in the isolation of these mutations. The probable gene order on linkage group VII is: centromere, relB, couA, bsgB, cobA, relA . Maintenance of aneuploids disomic for linkage group VII was made possible by complementation of a rel mutation on each linkage group VII homologue by the corresponding wild-type allele on the other linkage group VII homologue. The duplication-bearing disomic strains were slow-growing and produced faster-growing sectors on the colony edge. Haploid sectors probably arise by a combination of mitotic recombination and subsequent loss of one homologue, diploid sectors may be formed by chromosome doubling to 2n + 2, followed by chromosome loss to return to 2n, and aneuploid sectors may arise by deletion or new mutation.
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Selected References
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- Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Welker D. L., Hirth K. P., Romans P., Noegel A., Firtel R. A., Williams K. L. The use of restriction fragment length polymorphisms and DNA duplications to study the organization of the actin multigene family in Dictyostelium discoideum. Genetics. 1986 Jan;112(1):27–42. doi: 10.1093/genetics/112.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Welker D. L., Metz B. A., Williams K. L. Chromosome rearrangements in Dictyostelium discoideum. Genetics. 1982 Dec;102(4):711–723. doi: 10.1093/genetics/102.4.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Welker D. L., Williams K. L. Genetic and cytological characterisation of fusion chromosomes of Dictyostelium discoideum. Chromosoma. 1981;82(3):321–332. doi: 10.1007/BF00285758. [DOI] [PubMed] [Google Scholar]
- Welker D. L., Williams K. L. The assignment of four new loci, including the coumarin sensitivity locus couA, to linkage group VII of Dictyostelium discoideum. J Gen Microbiol. 1980 Sep;120(1):149–159. doi: 10.1099/00221287-120-1-149. [DOI] [PubMed] [Google Scholar]