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. 1987 May;116(1):23–31. doi: 10.1093/genetics/116.1.23

The Constitutive, Glucose-Repression-Insensitive Mutation of the Yeast MAL4 Locus Is an Alteration of the MAL43 Gene

Maureen J Charron 1, Corinne A Michels 1
PMCID: PMC1203117  PMID: 3036644

Abstract

Mutations resulting in constitutive production of maltase have been identified at each of the five MAL loci of Saccharomyces yeasts. Here we examine a dominant constitutive, glucose-repression-insensitive allele of the MAL4 locus (MAL4-C). Our results demonstrate that MAL4-C is an alteration in the MAL43 gene, which encodes the positive regulator of the MAL structural genes, and that its product is trans-acting. The MAL43 gene from the MAL4-C strain was cloned and integrated into a series of nonfermenting strains lacking a functional regulatory gene but carrying copies of the maltose permease and maltase structural genes. Expression of the maltase structural gene was both constitutive and insensitive to glucose repression in these transformants. The MAL4-C allele also results in constitutive expression of the unlinked MAL12 gene (encoding maltase) in this strain. In addition, the cloned MAL43 gene was shown to be dominant to the wild-type MAL63 gene. We also show that most of the glucose repression insensitivity of strains carrying the MAL4-C allele results from alteration of MAL43.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Charron M. J., Dubin R. A., Michels C. A. Structural and functional analysis of the MAL1 locus of Saccharomyces cerevisiae. Mol Cell Biol. 1986 Nov;6(11):3891–3899. doi: 10.1128/mcb.6.11.3891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clarke L., Carbon J. Isolation of the centromere-linked CDC10 gene by complementation in yeast. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2173–2177. doi: 10.1073/pnas.77.4.2173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen J. D., Goldenthal M. J., Chow T., Buchferer B., Marmur J. Organization of the MAL loci of Saccharomyces. Physical identification and functional characterization of three genes at the MAL6 locus. Mol Gen Genet. 1985;200(1):1–8. doi: 10.1007/BF00383304. [DOI] [PubMed] [Google Scholar]
  4. Dawes I. W., Hardie I. D. Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether. Mol Gen Genet. 1974;131(4):281–289. doi: 10.1007/BF00264859. [DOI] [PubMed] [Google Scholar]
  5. Dubin R. A., Needleman R. B., Gossett D., Michels C. A. Identification of the structural gene encoding maltase within the MAL6 locus of Saccharomyces carlsbergensis. J Bacteriol. 1985 Nov;164(2):605–610. doi: 10.1128/jb.164.2.605-610.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dubin R. A., Perkins E. L., Needleman R. B., Michels C. A. Identification of a second trans-acting gene controlling maltose fermentation in Saccharomyces carlsbergensis. Mol Cell Biol. 1986 Aug;6(8):2757–2765. doi: 10.1128/mcb.6.8.2757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hinnen A., Hicks J. B., Fink G. R. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. doi: 10.1073/pnas.75.4.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. doi: 10.1128/jb.153.1.163-168.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Matsumoto K., Adachi Y., Toh-e A., Oshima Y. Function of positive regulatory gene gal4 in the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae: evidence that the GAL81 region codes for part of the gal4 protein. J Bacteriol. 1980 Feb;141(2):508–527. doi: 10.1128/jb.141.2.508-527.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matsumoto K., Yoshimatsu T., Oshima Y. Recessive mutations conferring resistance to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae. J Bacteriol. 1983 Mar;153(3):1405–1414. doi: 10.1128/jb.153.3.1405-1414.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mortimer R. K., Hawthorne D. C. Genetic mapping in Saccharomyces. Genetics. 1966 Jan;53(1):165–173. doi: 10.1093/genetics/53.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Needleman R. B., Kaback D. B., Dubin R. A., Perkins E. L., Rosenberg N. G., Sutherland K. A., Forrest D. B., Michels C. A. MAL6 of Saccharomyces: a complex genetic locus containing three genes required for maltose fermentation. Proc Natl Acad Sci U S A. 1984 May;81(9):2811–2815. doi: 10.1073/pnas.81.9.2811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Needleman R. B., Michels C. Repeated family of genes controlling maltose fermentation in Saccharomyces carlsbergensis. Mol Cell Biol. 1983 May;3(5):796–802. doi: 10.1128/mcb.3.5.796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Orr-Weaver T. L., Szostak J. W., Rothstein R. J. Genetic applications of yeast transformation with linear and gapped plasmids. Methods Enzymol. 1983;101:228–245. doi: 10.1016/0076-6879(83)01017-4. [DOI] [PubMed] [Google Scholar]
  15. Ouerdane Y, Denis A, Désesquelles J. Time evolution of alignment and orientation in an electric field for the n 1D2 He I levels (3 <= n <= 5). Phys Rev A Gen Phys. 1986 Sep;34(3):1966–1976. doi: 10.1103/physreva.34.1966. [DOI] [PubMed] [Google Scholar]
  16. Ouwehand J., van Wijk R. Regulation of maltase and -methylglucosidase synthesis in genetically defined strains of Saccharomyces carlsbergensis. Mol Gen Genet. 1972;117(1):30–38. doi: 10.1007/BF00268834. [DOI] [PubMed] [Google Scholar]
  17. Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]
  18. Van Wijk R., Ouwehand J., van den Bos T., Koningsberger V. V. Induction and catabolite repression of alpha-glucosidase synthesis in protoplasts of Saccharomyces carlsbergensis. Biochim Biophys Acta. 1969 Jul 22;186(1):178–191. doi: 10.1016/0005-2787(69)90501-2. [DOI] [PubMed] [Google Scholar]
  19. ten Berge A. M., Zoutewelle G., van de Poll K. W., Bloemers H. P. Regulation of maltose fermentation in Saccharomyces carlsbergensis. II. Properties of a constitutive MAL6-mutant. Mol Gen Genet. 1973 Sep 5;125(2):139–146. doi: 10.1007/BF00268867. [DOI] [PubMed] [Google Scholar]
  20. ten Berge A. M., Zoutewelle G., van de Poll K. W. Regulation of maltose fermentation in Saccharomyces carlsbergensis. I. The function of the gene MAL6, as recognized by mal6-mutants. Mol Gen Genet. 1973 Jul 2;123(3):233–246. doi: 10.1007/BF00271242. [DOI] [PubMed] [Google Scholar]

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