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. 1987 Jul;116(3):359–369. doi: 10.1093/genetics/116.3.359

Physical Analysis of Tn10- and Is10-Promoted Transpositions and Rearrangements

Michael M Shen 1, Elisabeth A Raleigh 1, Nancy Kleckner 1
PMCID: PMC1203147  PMID: 3038673

Abstract

We have investigated by Southern blot hybridization the rate of IS10 transposition and other Tn10/IS10-promoted rearrangements in Escherichia coli and Salmonella strains bearing single chromosomal insertions of Tn10 or a related Tn 10 derivative. We present evidence for three primary conclusions. First, the rate of IS10 transposition is approximately 10-4 per cell per bacterial generation when overnight cultures are grown and plated on minimal media and is at least ten times more frequent than any other Tn 10/IS10-promoted DNA alteration. Second, all of the chromosomal rearrangements observed can be accounted for by two previously characterized Tn10-promoted rearrangements: deletion/inversions and deletions. Together these rearrangements occur at about 10% the rate of IS10 transposition. Third, the data suggest that intramolecular Tn10-promoted rearrangements preferentially use nearby target sites, while the target sites for IS 10 transposition events are scattered randomly around the chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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