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. 1988 Jan;118(1):21–29. doi: 10.1093/genetics/118.1.21

DNA Sequence Determinants of λ Repressor Binding in Vivo

N Benson 1, P Sugiono 1, P Youderian 1
PMCID: PMC1203262  PMID: 8608928

Abstract

The critical operator determinants for λ repressor recognition have been defined by analyzing the binding of wild-type repressor to a set of mutant operators in vivo. Base pair substitutions at six positions within the λ operator half-site impair binding severely, and define these base pairs as critical for operator function. One mutant operator binds repressor better than the consensus operator, and is a superoperator. The model proposed by M. Lewis in 1983 for the binding of λ repressor to its operator accurately predicts the observed operator requirements for binding in vivo, with several minor exceptions. The order of affinities of the six natural λ operators has also been determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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