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. 1988 Jan;118(1):41–47. doi: 10.1093/genetics/118.1.41

Uga Suppressors in Saccharomyces Cerevisiae: Allelism, Action Spectra and Map Positions

B I Ono 1, R Fujimoto 1, Y Ohno 1, N Maeda 1, Y Tsuchiya 1, T Usui 1, Y Ishino-Arao 1
PMCID: PMC1203264  PMID: 8608930

Abstract

Sixty independent UGA suppressors of Saccharomyces cerevisiae have been studied. They are dominant and are divided into 16 groups (loci) by recombination. Suppressors representing these loci are divided into two classes by action spectra; four in class 1 (a broad action spectrum) and 12 in class 2 (a narrow action spectrum). Class 1 suppressors are less frequent in terms of not only total number but also number per locus than class 2 suppressors, indicating difference in either or both mutation frequency and selective pressure between suppressors of the two classes. Two of the class 1 suppressors, SUP152 and SUP161, do not recombine with SUP28 and SUP33, leucine-inserting UAA suppressors, respectively, indicating that they are mutations in genes coding for tRNA(UUA)(Leu). Of the remaining two class 1 suppressors, SUP160 which causes lethality in the ψ(+) cytoplasm is mapped on chromosome XV very close to the centromere, and SUP165 on the right arm of chromosome XIV 44 cM distal to lys9. Of the class 2 suppressors, ten do not recombine with one or another of previously known UGA suppressors. The remaining two class 2 suppressors, SUP154 and SUP155, are mapped on the left and right arms of chromosome VII, respectively.

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Selected References

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  1. Chattoo B. B., Palmer E., Ono B., Sherman F. Patterns of Genetic and Phenotypic Suppression of lys2 Mutations in the Yeast SACCHAROMYCES CEREVISIAE. Genetics. 1979 Sep;93(1):67–79. doi: 10.1093/genetics/93.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HAWTHORNE D. C., MORTIMER R. K. Super-suppressors in yeast. Genetics. 1963 Apr;48:617–620. doi: 10.1093/genetics/48.4.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hawthorne D. C., Leupold U. Suppressors in yeast. Curr Top Microbiol Immunol. 1974;64(0):1–47. doi: 10.1007/978-3-642-65848-8_1. [DOI] [PubMed] [Google Scholar]
  4. Hawthorne D. C. UGA mutations and UGA suppressors in yeast. Biochimie. 1976;58(1-2):179–182. doi: 10.1016/s0300-9084(76)80368-9. [DOI] [PubMed] [Google Scholar]
  5. Hottinger H., Pearson D., Yamao F., Gamulin V., Cooley L., Cooper T., Söll D. Nonsense suppression in Schizosaccharomyces pombe: the S. pombe Sup3-e tRNASerUGA gene is active in S. cerevisiae. Mol Gen Genet. 1982;188(2):219–224. doi: 10.1007/BF00332678. [DOI] [PubMed] [Google Scholar]
  6. Liebman S. W., Sherman F. Extrachromosomal psi+ determinant suppresses nonsense mutations in yeast. J Bacteriol. 1979 Sep;139(3):1068–1071. doi: 10.1128/jb.139.3.1068-1071.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mortimer R. K., Hawthorne D. C. Genetic mapping in yeast. Methods Cell Biol. 1975;11:221–233. doi: 10.1016/s0091-679x(08)60325-8. [DOI] [PubMed] [Google Scholar]
  8. Mortimer R. K., Schild D. Genetic map of Saccharomyces cerevisiae. Microbiol Rev. 1980 Dec;44(4):519–571. doi: 10.1128/mr.44.4.519-571.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ono B. I., Ishino Y., Shinoda S. Nonsense mutations in the can1 locus of Saccharomyces cerevisiae. J Bacteriol. 1983 Jun;154(3):1476–1479. doi: 10.1128/jb.154.3.1476-1479.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ono B. I., Stewart J. W., Sherman F. Yeast UAA suppressors effective in psi+ strains serine-inserting suppressors. J Mol Biol. 1979 Feb 15;128(1):81–100. doi: 10.1016/0022-2836(79)90309-7. [DOI] [PubMed] [Google Scholar]
  11. Ono B. I., Stewart J. W., Sherman F. Yeast UAA suppressors effective in psi+ strains: leucine-inserting suppressors. J Mol Biol. 1979 Aug 15;132(3):507–520. doi: 10.1016/0022-2836(79)90272-9. [DOI] [PubMed] [Google Scholar]
  12. Ono B. I., Wills N., Stewart J. W., Gesteland R. F., Sherman F. Serine-inserting UAA suppression mediated by yeast tRNASer. J Mol Biol. 1981 Aug 15;150(3):361–373. doi: 10.1016/0022-2836(81)90552-0. [DOI] [PubMed] [Google Scholar]
  13. Ono B., Ishino-Arao Y., Tanaka M., Awano I., Shinoda S. Recessive nonsense suppressors in Saccharomyces cerevisiae: action spectra, complementation groups and map positions. Genetics. 1986 Oct;114(2):363–374. doi: 10.1093/genetics/114.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perkins D. D. Biochemical Mutants in the Smut Fungus Ustilago Maydis. Genetics. 1949 Sep;34(5):607–626. doi: 10.1093/genetics/34.5.607. [DOI] [PMC free article] [PubMed] [Google Scholar]

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