Abstract
We have investigated the chromosomal region around unc-60 V, a gene affecting muscle structure, in the nematode Caenorhabditis elegans. The region studied covers 3 map units and lies at the left end of linkage group (LG) V. Compared to the region around dpy-11 (at the center of LGV), the unc-60 region has relatively few visible genes per map unit. We found the same to be true for essential genes. By screening simultaneously for recessive lethals closely linked to either dpy-11 or unc-60, we recovered ethyl methanesulfonate-induced mutations in 10 essential genes near dpy-11 but in only two genes near unc-60. Four deficiency breakpoints were mapped to the unc-60 region. Using recombination and deficiency mapping we established the following gene order: let-336, unc-34, let-326, unc-60, emb-29, let-426. Regarding unc-60 itself, we compared the effect of ten alleles (including five isolated during this study) on hermaphrodite mobility and fecundity. We used intragenic mapping to position eight of these alleles. The results show that these alleles are not distributed uniformly within the gene, but map to two groups approximately 0.012 map unit apart.
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- Anderson P., Brenner S. A selection for myosin heavy chain mutants in the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4470–4474. doi: 10.1073/pnas.81.14.4470. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974 May;77(1):71–94. doi: 10.1093/genetics/77.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cassada R., Isnenghi E., Culotti M., von Ehrenstein G. Genetic analysis of temperature-sensitive embryogenesis mutants in Caenorhabditis elegans. Dev Biol. 1981 May;84(1):193–205. doi: 10.1016/0012-1606(81)90383-3. [DOI] [PubMed] [Google Scholar]
- Clark S. H., McCarron M., Love C., Chovnick A. On the identification of the rosy locus DNA in Drosophila melanogaster: intragenic recombination mapping of mutations associated with insertions and deletions. Genetics. 1986 Apr;112(4):755–767. doi: 10.1093/genetics/112.4.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hecht R. M., Berg-Zabelshansky M., Rao P. N., Davis F. M. Conditional absence of mitosis-specific antigens in a temperature-sensitive embryonic-arrest mutant of Caenorhabditis elegans. J Cell Sci. 1987 Mar;87(Pt 2):305–314. doi: 10.1242/jcs.87.2.305. [DOI] [PubMed] [Google Scholar]
- Herman R. K., Kari C. K., Hartman P. S. Dominant X-chromosome nondisjunction mutants of Caenorhabditis elegans. Genetics. 1982 Nov;102(3):379–400. doi: 10.1093/genetics/102.3.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herman R. K., Madl J. E., Kari C. K. Duplications in Caenorhabditis elegans. Genetics. 1979 Jun;92(2):419–435. doi: 10.1093/genetics/92.2.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hilliker A. J., Chovnick A. Further observations on intragenic recombination in Drosophila melanogaster. Genet Res. 1981 Dec;38(3):281–296. doi: 10.1017/s0016672300020619. [DOI] [PubMed] [Google Scholar]
- Horvitz H. R., Brenner S., Hodgkin J., Herman R. K. A uniform genetic nomenclature for the nematode Caenorhabditis elegans. Mol Gen Genet. 1979 Sep;175(2):129–133. doi: 10.1007/BF00425528. [DOI] [PubMed] [Google Scholar]
- Judd B. H., Shen M. W., Kaufman T. C. The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics. 1972 May;71(1):139–156. doi: 10.1093/genetics/71.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lawrence P. A., Morata G. The elements of the bithorax complex. Cell. 1983 Dec;35(3 Pt 2):595–601. doi: 10.1016/0092-8674(83)90091-0. [DOI] [PubMed] [Google Scholar]
- Meneely P. M., Herman R. K. Lethals, steriles and deficiencies in a region of the X chromosome of Caenorhabditis elegans. Genetics. 1979 May;92(1):99–115. doi: 10.1093/genetics/92.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meneely P. M., Herman R. K. Suppression and function of X-linked lethal and sterile mutations in Caenorhabditis elegans. Genetics. 1981 Jan;97(1):65–84. doi: 10.1093/genetics/97.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moerman D. G., Baillie D. L. Genetic Organization in CAENORHABDITIS ELEGANS: Fine-Structure Analysis of the unc-22 Gene. Genetics. 1979 Jan;91(1):95–103. doi: 10.1093/genetics/91.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Park E. C., Horvitz H. R. C. elegans unc-105 mutations affect muscle and are suppressed by other mutations that affect muscle. Genetics. 1986 Aug;113(4):853–867. doi: 10.1093/genetics/113.4.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riddle D. L., Brenner S. Indirect suppression in Caenorhabditis elegans. Genetics. 1978 Jun;89(2):299–314. doi: 10.1093/genetics/89.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rogalski T. M., Baillie D. L. Genetic organization of the unc-22 IV gene and the adjacent region in Caenorhabditis elegans. Mol Gen Genet. 1985;201(3):409–414. doi: 10.1007/BF00331331. [DOI] [PubMed] [Google Scholar]
- Rogalski T. M., Moerman D. G., Baillie D. L. Essential genes and deficiencies in the unc-22 IV region of Caenorhabditis elegans. Genetics. 1982 Dec;102(4):725–736. doi: 10.1093/genetics/102.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rose A. M., Baillie D. L. Genetic organization of the region around UNC-15 (I), a gene affecting paramyosin in Caenorhabditis elegans. Genetics. 1980 Nov;96(3):639–648. doi: 10.1093/genetics/96.3.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rose A. M., Baillie D. L. The Effect of Temperature and Parental Age on Recombination and Nondisjunction in CAENORHABDITIS ELEGANS. Genetics. 1979 Jun;92(2):409–418. doi: 10.1093/genetics/92.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenbluth R. E., Baillie D. L. The genetic analysis of a reciprocal translocation, eT1(III; V), in Caenorhabditis elegans. Genetics. 1981 Nov-Dec;99(3-4):415–428. doi: 10.1093/genetics/99.3-4.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenbluth R. E., Cuddeford C., Baillie D. L. Mutagenesis in Caenorhabditis elegans. II. A spectrum of mutational events induced with 1500 r of gamma-radiation. Genetics. 1985 Mar;109(3):493–511. doi: 10.1093/genetics/109.3.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sigurdson D. C., Spanier G. J., Herman R. K. Caenorhabditis elegans deficiency mapping. Genetics. 1984 Oct;108(2):331–345. doi: 10.1093/genetics/108.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trent C., Tsuing N., Horvitz H. R. Egg-laying defective mutants of the nematode Caenorhabditis elegans. Genetics. 1983 Aug;104(4):619–647. doi: 10.1093/genetics/104.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waterston R. H. A second informational suppressor, SUP-7 X, in Caenorhabditis elegans. Genetics. 1981 Feb;97(2):307–325. doi: 10.1093/genetics/97.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waterston R. H., Fishpool R. M., Brenner S. Mutants affecting paramyosin in Caenorhabditis elegans. J Mol Biol. 1977 Dec 15;117(3):679–697. doi: 10.1016/0022-2836(77)90064-x. [DOI] [PubMed] [Google Scholar]
- Waterston R. H., Thomson J. N., Brenner S. Mutants with altered muscle structure of Caenorhabditis elegans. Dev Biol. 1980 Jun 15;77(2):271–302. doi: 10.1016/0012-1606(80)90475-3. [DOI] [PubMed] [Google Scholar]
- Wills N., Gesteland R. F., Karn J., Barnett L., Bolten S., Waterston R. H. The genes sup-7 X and sup-5 III of C. elegans suppress amber nonsense mutations via altered transfer RNA. Cell. 1983 Jun;33(2):575–583. doi: 10.1016/0092-8674(83)90438-5. [DOI] [PubMed] [Google Scholar]
- Woodruff R. C., Ashburner M. The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. II. Lethal mutations in the region. Genetics. 1979 May;92(1):133–149. doi: 10.1093/genetics/92.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]