Abstract
This report presents the results of a recombination experiment designed to question the existence of special sites for the initiation or termination of a recombination heteroduplex within the region of the rosy locus. Intragenic recombination events were monitored between two physically separated rosy mutant alleles ry(301) and ry(2) utilizing DNA restriction site polymorphisms as genetic markers. Both ry(301) and ry(2) are known from previous studies to be associated with gene conversion frequencies an order of magnitude lower than single site mutations. The mutations are associated with large, well defined insertions located as internal sites within the locus in prior intragenic mapping studies. On the molecular map, they represent large insertions approximately 2.7 kb apart in the second and third exons, respectively, of the XDH coding region. The present study monitors intragenic recombination in a mutant heterozygous genotype in which DNA homology is disrupted by these large discontinuities, greater than the region of DNA homology and flanking both sides of the locus. If initiation/or termination requires separate sites at either end of the locus, then intragenic recombination within the rosy locus of the heterozygote should be eliminated. Contrary to expectation, significant recombination between these sites is seen.
Full Text
The Full Text of this article is available as a PDF (2.5 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Carpenter A. T. Mismatch repair, gene conversion, and crossing-over in two recombination-defective mutants of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5961–5965. doi: 10.1073/pnas.79.19.5961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chovnick A., Ballantyne G. H., Holm D. G. Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster. Genetics. 1971 Oct;69(2):179–209. doi: 10.1093/genetics/69.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chovnick A. Gene conversion and transfer of genetic information within the inverted region of inversion heterozygotes. Genetics. 1973 Sep;75(1):123–131. doi: 10.1093/genetics/75.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark S. H., Chovnick A. Studies of normal and position-affected expression of rosy region genes in Drosophila melanogaster. Genetics. 1986 Nov;114(3):819–840. doi: 10.1093/genetics/114.3.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark S. H., Daniels S., Rushlow C. A., Hilliker A. J., Chovnick A. Tissue-specific and pretranslational character of variants of the rosy locus control element in Drosophila melanogaster. Genetics. 1984 Dec;108(4):953–968. doi: 10.1093/genetics/108.4.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark S. H., Hilliker A. J., Chovnick A. Genetic analysis of the right (3') end of the rosy locus in Drosophila melanogaster. Genet Res. 1986 Apr;47(2):109–116. doi: 10.1017/s001667230002293x. [DOI] [PubMed] [Google Scholar]
- Clark S. H., McCarron M., Love C., Chovnick A. On the identification of the rosy locus DNA in Drosophila melanogaster: intragenic recombination mapping of mutations associated with insertions and deletions. Genetics. 1986 Apr;112(4):755–767. doi: 10.1093/genetics/112.4.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coté B., Bender W., Curtis D., Chovnick A. Molecular mapping of the rosy locus in Drosophila melanogaster. Genetics. 1986 Apr;112(4):769–783. doi: 10.1093/genetics/112.4.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981 Apr;112(2):295–298. doi: 10.1016/0003-2697(81)90296-7. [DOI] [PubMed] [Google Scholar]
- Gelbart W. M., McCarron M., Pandey J., Chovnick A. Genetic limits of the xanthine dehydrogenase structural element within the rosy locus in Drosophila melanogaster. Genetics. 1974 Nov;78(3):869–886. doi: 10.1093/genetics/78.3.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gelbart W., McCarron M., Chovnick A. Extension of the limits of the XDH structural element in Drosophila melanogaster. Genetics. 1976 Oct;84(2):211–232. doi: 10.1093/genetics/84.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hilliker A. J., Chovnick A. Further observations on intragenic recombination in Drosophila melanogaster. Genet Res. 1981 Dec;38(3):281–296. doi: 10.1017/s0016672300020619. [DOI] [PubMed] [Google Scholar]
- Keith T. P., Riley M. A., Kreitman M., Lewontin R. C., Curtis D., Chambers G. Sequence of the structural gene for xanthine dehydrogenase (rosy locus) in Drosophila melanogaster. Genetics. 1987 May;116(1):67–73. doi: 10.1093/genetics/116.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee C. S., Curtis D., McCarron M., Love C., Gray M., Bender W., Chovnick A. Mutations affecting expression of the rosy locus in Drosophila melanogaster. Genetics. 1987 May;116(1):55–66. doi: 10.1093/genetics/116.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MURRAY N. E. Polarized recombination and fine structure within the me-2 gene of Neurospora crassa. Genetics. 1963 Sep;48:1163–1183. doi: 10.1093/genetics/48.9.1163. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meselson M. S., Radding C. M. A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975 Jan;72(1):358–361. doi: 10.1073/pnas.72.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Rushlow C. A., Bender W., Chovnick A. Studies on the mechanism of heterochromatic position effect at the rosy locus of Drosophila melanogaster. Genetics. 1984 Nov;108(3):603–615. doi: 10.1093/genetics/108.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Szostak J. W., Orr-Weaver T. L., Rothstein R. J., Stahl F. W. The double-strand-break repair model for recombination. Cell. 1983 May;33(1):25–35. doi: 10.1016/0092-8674(83)90331-8. [DOI] [PubMed] [Google Scholar]