Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1988 Mar;118(3):401–410. doi: 10.1093/genetics/118.3.401

Physical Lengths of Meiotic and Mitotic Gene Conversion Tracts in Saccharomyces Cerevisiae

S R Judd 1, T D Petes 1
PMCID: PMC1203294  PMID: 2835285

Abstract

Physical lengths of gene conversion tracts for meiotic and mitotic conversions were examined, using the same diploid yeast strain in all experiments. This strain is heterozygous for a mutation in the URA3 gene as well as closely linked restriction site markers. In cells that had a gene conversion event at the URA3 locus, it was determined by Southern analysis which of the flanking heterozygous restriction sites had co-converted. It was found that mitotic conversion tracts were longer on the average than meiotic tracts. About half of the tracts generated by spontaneous mitotic gene conversion included heterozygous markers 4.2 kb apart; none of the meiotic conversions included these markers. Stimulation of mitotic gene conversion by ultraviolet light or methylmethanesulfonate had no obvious effect on the size or distribution of the tracts. Almost all conversion tracts were continuous.

Full Text

The Full Text of this article is available as a PDF (3.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahn B. Y., Livingston D. M. Mitotic gene conversion lengths, coconversion patterns, and the incidence of reciprocal recombination in a Saccharomyces cerevisiae plasmid system. Mol Cell Biol. 1986 Nov;6(11):3685–3693. doi: 10.1128/mcb.6.11.3685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop D. K., Kolodner R. D. Repair of heteroduplex plasmid DNA after transformation into Saccharomyces cerevisiae. Mol Cell Biol. 1986 Oct;6(10):3401–3409. doi: 10.1128/mcb.6.10.3401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boeke J. D., LaCroute F., Fink G. R. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet. 1984;197(2):345–346. doi: 10.1007/BF00330984. [DOI] [PubMed] [Google Scholar]
  4. Dicarprio L., Hastings P. J. Gene conversion and intragenic recombination at the SUP6 locus and the surrounding region in Saccharomyces cerevisiae. Genetics. 1976 Dec;84(4):697–721. doi: 10.1093/genetics/84.4.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Esposito M. S. Evidence that spontaneous mitotic recombination occurs at the two-strand stage. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4436–4440. doi: 10.1073/pnas.75.9.4436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fogel S., Mortimer R. K. Informational transfer in meiotic gene conversion. Proc Natl Acad Sci U S A. 1969 Jan;62(1):96–103. doi: 10.1073/pnas.62.1.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Golin J. E., Esposito M. S. Coincident gene conversion during mitosis in saccharomyces. Genetics. 1984 Jul;107(3):355–365. doi: 10.1093/genetics/107.3.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Golin J. E., Esposito M. S. Mitotic recombination: mismatch correction and replicational resolution of Holliday structures formed at the two strand stage in Saccharomyces. Mol Gen Genet. 1981;183(2):252–263. doi: 10.1007/BF00270626. [DOI] [PubMed] [Google Scholar]
  9. Golin J. E., Falco S. C., Margolskee J. P. Coincident gene conversion events in yeast that involve a large insertion. Genetics. 1986 Dec;114(4):1081–1094. doi: 10.1093/genetics/114.4.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jinks-Robertson S., Petes T. D. Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics. 1986 Nov;114(3):731–752. doi: 10.1093/genetics/114.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klein H. L. Lack of association between intrachromosomal gene conversion and reciprocal exchange. 1984 Aug 30-Sep 5Nature. 310(5980):748–753. doi: 10.1038/310748a0. [DOI] [PubMed] [Google Scholar]
  12. Klein H. L., Petes T. D. Intrachromosomal gene conversion in yeast. Nature. 1981 Jan 15;289(5794):144–148. doi: 10.1038/289144a0. [DOI] [PubMed] [Google Scholar]
  13. Kunz B. A., Haynes R. H. Phenomenology and genetic control of mitotic recombination in yeast. Annu Rev Genet. 1981;15:57–89. doi: 10.1146/annurev.ge.15.120181.000421. [DOI] [PubMed] [Google Scholar]
  14. Meselson M. S., Radding C. M. A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975 Jan;72(1):358–361. doi: 10.1073/pnas.72.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Montelone B. A., Prakash S., Prakash L. Spontaneous mitotic recombination in mms8-1, an allele of the CDC9 gene of Saccharomyces cerevisiae. J Bacteriol. 1981 Aug;147(2):517–525. doi: 10.1128/jb.147.2.517-525.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nakai S., Mortimer R. K. Studies on the genetic mechanism of radiation-induced mitotic segregation in yeast. Mol Gen Genet. 1969;103(4):329–338. doi: 10.1007/BF00383483. [DOI] [PubMed] [Google Scholar]
  17. Orr-Weaver T. L., Szostak J. W. Fungal recombination. Microbiol Rev. 1985 Mar;49(1):33–58. doi: 10.1128/mr.49.1.33-58.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Petes T. D., Hereford L. M., Botstein D. Simple Mendelian inheritance of the repeating yeast ribosomal DNA genes. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1201–1207. doi: 10.1101/sqb.1978.042.01.121. [DOI] [PubMed] [Google Scholar]
  19. Rose M., Grisafi P., Botstein D. Structure and function of the yeast URA3 gene: expression in Escherichia coli. Gene. 1984 Jul-Aug;29(1-2):113–124. doi: 10.1016/0378-1119(84)90172-0. [DOI] [PubMed] [Google Scholar]
  20. Szostak J. W., Orr-Weaver T. L., Rothstein R. J., Stahl F. W. The double-strand-break repair model for recombination. Cell. 1983 May;33(1):25–35. doi: 10.1016/0092-8674(83)90331-8. [DOI] [PubMed] [Google Scholar]
  21. White J. H., Lusnak K., Fogel S. Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate. Nature. 1985 May 23;315(6017):350–352. doi: 10.1038/315350a0. [DOI] [PubMed] [Google Scholar]
  22. Williamson V. M. Transposable elements in yeast. Int Rev Cytol. 1983;83:1–25. doi: 10.1016/s0074-7696(08)61684-8. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES