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. 1988 Apr;118(4):593–600. doi: 10.1093/genetics/118.4.593

Repair of Single Base-Pair Transversion Mismatches of Escherichia Coli in Vitro: Correction of Certain a/G Mismatches Is Independent of Dam Methylation and Host Muthls Gene Functions

A L Lu 1, D Y Chang 1
PMCID: PMC1203314  PMID: 3284785

Abstract

Six different base-pair transversion mismatches are repaired with different efficiencies in an in vitro mismatch repair system. In particular, the T/T and C/C mismatches appear to be less efficiently repaired than the A/A and G/G mismatches. Four A/G and four C/T mismatches at different positions are repaired to different extents. One of the A/G mismatches is repaired equally efficiently when DNA heteroduplexes are fully methylated or hemi-methylated at the d(GATC) sequences. This type of mismatch repair appears to be unidirectional with A to C conversion by acting at A/G mispairs to restore the C/G pairs. This methylation-independent correction is not controlled by the mutH, mutL, mutS, uvrE, uvrB, phr, recA, recF, and recJ gene products. The independence of the transversion mismatch repair of these genes and methylation distinguishes this from the known mismatch repair pathways.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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