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. 1988 Apr;118(4):601–607. doi: 10.1093/genetics/118.4.601

Nonsense Mutations in Essential Genes of Saccharomyces Cerevisiae

L Riles 1, M V Olson 1
PMCID: PMC1203315  PMID: 3284786

Abstract

A new method for isolating nonsense mutations in essential yeast genes has been used to develop a collection of 115 ochre mutations that define 94 complementation groups. The mutants are isolated in a genetic background that includes an ochre suppressor on a metastable plasmid and a suppressible colony-color marker on a chromosome. When the parental strain is plated on a rich medium, the colonies display a pattern of red, plasmid-free sectors on a white background. Mutants containing an ochre mutation in any essential yeast gene give rise to nonsectoring, white colonies, since cell growth is dependent on the presence of the plasmid-borne suppressor. Analysis of the data suggests that mutations are being recovered from a pool of approximately 250 genes.

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Selected References

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