Abstract
Levels of DNA divergence among the eight species of the Drosophila melanogaster subgroup and D. takahashii have been determined using the technique of DNA-DNA hybridization. Two types of DNA were used: single-copy nuclear DNA (scnDNA) and mitochondrial DNA (mtDNA). The major findings are: (1) A phylogeny has been derived for the group based on scnDNA which is congruent with chromosomal data, morphology, and behavior. The three homosequential species, simulans, sechellia, and mauritiana, are very closely related; the scnDNA divergence indicate the two island species are a monophyletic group. (2) The rates of change of scnDNA and mtDNA are not greatly different; if anything scnDNA evolves faster than mtDNA. (3) The rates of scnDNA evolution are not closely correlated to chromosomal (inversion) evolution. (4) The Drosophila genome appears to consist of two distinct classes of scnDNA with respect to rate of evolutionary change, a very rapidly evolving fraction and a relatively conservative fraction. (5) The absolute rate of change was estimated to be at least 1.7% nucleotide substitution per one million years. (6) DNA distance estimates based on restriction site variation are correlated with distances based on DNA-DNA hybridization, although the correlation is not very strong.
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Selected References
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- Britten R. J., Cetta A., Davidson E. H. The single-copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus. Cell. 1978 Dec;15(4):1175–1186. doi: 10.1016/0092-8674(78)90044-2. [DOI] [PubMed] [Google Scholar]
- Britten R. J. Rates of DNA sequence evolution differ between taxonomic groups. Science. 1986 Mar 21;231(4744):1393–1398. doi: 10.1126/science.3082006. [DOI] [PubMed] [Google Scholar]
- Caccone A., Amato G. D., Powell J. R. Intraspecific DNA divergence in Drosophila: a study on parthenogenetic D. mercatorum. Mol Biol Evol. 1987 Jul;4(4):343–350. doi: 10.1093/oxfordjournals.molbev.a040451. [DOI] [PubMed] [Google Scholar]
- Fitch W. M., Margoliash E. Construction of phylogenetic trees. Science. 1967 Jan 20;155(3760):279–284. doi: 10.1126/science.155.3760.279. [DOI] [PubMed] [Google Scholar]
- Hunt J. A., Hall T. J., Britten R. J. Evolutionary distances in Hawaiian Drosophila measured by DNA reassociation. J Mol Evol. 1981;17(6):361–367. doi: 10.1007/BF01734358. [DOI] [PubMed] [Google Scholar]
- Martin C. H., Meyerowitz E. M. Characterization of the boundaries between adjacent rapidly and slowly evolving genomic regions in Drosophila. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8654–8658. doi: 10.1073/pnas.83.22.8654. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nei M., Li W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269–5273. doi: 10.1073/pnas.76.10.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nei M., Tajima F. DNA polymorphism detectable by restriction endonucleases. Genetics. 1981 Jan;97(1):145–163. doi: 10.1093/genetics/97.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schulze D. H., Lee C. S. DNA sequence comparison among closely related Drosophila species in the mulleri complex. Genetics. 1986 Jun;113(2):287–303. doi: 10.1093/genetics/113.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolstenholme D. R., Clary D. O. Sequence evolution of Drosophila mitochondrial DNA. Genetics. 1985 Apr;109(4):725–744. doi: 10.1093/genetics/109.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zwiebel L. J., Cohn V. H., Wright D. R., Moore G. P. Evolution of single-copy DNA and the ADH gene in seven drosophilids. J Mol Evol. 1982;19(1):62–71. doi: 10.1007/BF02100224. [DOI] [PubMed] [Google Scholar]