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. 1988 May;119(1):75–83. doi: 10.1093/genetics/119.1.75

P Element Insertions and Rearrangements at the Singed Locus of Drosophila Melanogaster

H Roiha 1, G M Rubin 1, K O'Hare 1
PMCID: PMC1203347  PMID: 2840331

Abstract

DNA from the singed gene of Drosophila melanogaster was isolated using an inversion between a previously cloned P element at cytological location 17C and the hypermutable allele singed-weak. Five out of nine singed mutants examined have alterations in their DNA maps in this region. The singed locus is a hotspot for mutation during P-M hybrid dysgenesis, and we have analyzed 22 mutations induced by P-M hybrid dysgenesis. All 22 have a P element inserted within a 700-bp region. The precise positions of 10 P element insertions were determined and they define 4 sites within a 100-bp interval. During P-M hybrid dysgenesis, the singed-weak allele is destabilized, producing two classes of phenotypically altered derivatives at high frequency. In singed-weak, two defective P elements are present in a ``head-to-head'' or inverse tandem arrangement. Excision of one element results in a more extreme singed bristle phenotype while excision of the other leads to a wild-type bristle phenotype.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bender H A. Studies on the Expression of Various Singed Alleles in Drosophila Melanogaster. Genetics. 1960 Jul;45(7):867–883. doi: 10.1093/genetics/45.7.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bingham P. M., Kidwell M. G., Rubin G. M. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982 Jul;29(3):995–1004. doi: 10.1016/0092-8674(82)90463-9. [DOI] [PubMed] [Google Scholar]
  3. Chia W., Howes G., Martin M., Meng Y. B., Moses K., Tsubota S. Molecular analysis of the yellow locus of Drosophila. EMBO J. 1986 Dec 20;5(13):3597–3605. doi: 10.1002/j.1460-2075.1986.tb04688.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Digan M. E., Haynes S. R., Mozer B. A., Dawid I. B., Forquignon F., Gans M. Genetic and molecular analysis of fs(1)h, a maternal effect homeotic gene in Drosophila. Dev Biol. 1986 Mar;114(1):161–169. doi: 10.1016/0012-1606(86)90392-1. [DOI] [PubMed] [Google Scholar]
  5. Engels W. R. A trans-acting product needed for P factor transposition in Drosophila. Science. 1984 Dec 7;226(4679):1194–1196. doi: 10.1126/science.6095450. [DOI] [PubMed] [Google Scholar]
  6. Engels W. R. Extrachromosomal control of mutability in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4011–4015. doi: 10.1073/pnas.76.8.4011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Engels W. R., Preston C. R. Identifying P factors in Drosophila by means of chromosome breakage hotspots. Cell. 1981 Nov;26(3 Pt 1):421–428. doi: 10.1016/0092-8674(81)90211-7. [DOI] [PubMed] [Google Scholar]
  8. Engels W. R. The P family of transposable elements in Drosophila. Annu Rev Genet. 1983;17:315–344. doi: 10.1146/annurev.ge.17.120183.001531. [DOI] [PubMed] [Google Scholar]
  9. Finnegan D. J., Fawcett D. H. Transposable elements in Drosophila melanogaster. Oxf Surv Eukaryot Genes. 1986;3:1–62. [PubMed] [Google Scholar]
  10. Green M. M. Genetic instability in Drosophila melanogaster: De novo induction of putative insertion mutations. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3490–3493. doi: 10.1073/pnas.74.8.3490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green M. M. Genetic instability in Drosophila melanogaster: the genetics of an MR element that makes complete P insertion mutations. Proc Natl Acad Sci U S A. 1986 Feb;83(4):1036–1040. doi: 10.1073/pnas.83.4.1036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kelley M. R., Kidd S., Berg R. L., Young M. W. Restriction of P-element insertions at the Notch locus of Drosophila melanogaster. Mol Cell Biol. 1987 Apr;7(4):1545–1548. doi: 10.1128/mcb.7.4.1545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Levis R., Bingham P. M., Rubin G. M. Physical map of the white locus of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Jan;79(2):564–568. doi: 10.1073/pnas.79.2.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maniatis T., Hardison R. C., Lacy E., Lauer J., O'Connell C., Quon D., Sim G. K., Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. doi: 10.1016/0092-8674(78)90036-3. [DOI] [PubMed] [Google Scholar]
  15. O'Hare K., Rubin G. M. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. doi: 10.1016/0092-8674(83)90133-2. [DOI] [PubMed] [Google Scholar]
  16. Perrimon N., Gans M. Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237. Dev Biol. 1983 Dec;100(2):365–373. doi: 10.1016/0012-1606(83)90231-2. [DOI] [PubMed] [Google Scholar]
  17. Rubin G. M., Kidwell M. G., Bingham P. M. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982 Jul;29(3):987–994. doi: 10.1016/0092-8674(82)90462-7. [DOI] [PubMed] [Google Scholar]
  18. Searles L. L., Greenleaf A. L., Kemp W. E., Voelker R. A. Sites of P element insertion and structures of P element deletions in the 5' region of Drosophila melanogaster RpII215. Mol Cell Biol. 1986 Oct;6(10):3312–3319. doi: 10.1128/mcb.6.10.3312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Simmons M. J., Lim J. K. Site specificity of mutations arising in dysgenic hybrids of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6042–6046. doi: 10.1073/pnas.77.10.6042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Spradling A. C., Rubin G. M. Transposition of cloned P elements into Drosophila germ line chromosomes. Science. 1982 Oct 22;218(4570):341–347. doi: 10.1126/science.6289435. [DOI] [PubMed] [Google Scholar]
  21. Tsubota S., Ashburner M., Schedl P. P-element-induced control mutations at the r gene of Drosophila melanogaster. Mol Cell Biol. 1985 Oct;5(10):2567–2574. doi: 10.1128/mcb.5.10.2567. [DOI] [PMC free article] [PubMed] [Google Scholar]

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