Skip to main content
NCBI home page
Genetics logoLink to Genetics
. 1988 Jun;119(2):345–353. doi: 10.1093/genetics/119.2.345

The Unc-22(iv) Region of Caenorhabditis Elegans: Genetic Analysis of Lethal Mutations

D V Clark 1, T M Rogalski 1, L M Donati 1, D L Baillie 1
PMCID: PMC1203417  PMID: 3396868

Abstract

The organization of essential genes in the unc-22 region, defined by the deficiency sDf2 on linkage group IV, has been studied. Using the balancer nT1 (IV;V), which suppresses recombination over 49 map units, 294 lethal mutations on LGIV(right) and LGV(left) were recovered using EMS mutagenesis. Twenty-six of these mutations fell into the unc-22 region. Together with previously isolated lethal mutations, there is now a total of 63 lethal mutations which fall into 31 complementation groups. Mutations were positioned on the map using eight overlapping deficiencies in addition to sDf2. The lethal alleles and deficiencies in the unc-22 region were characterized with respect to their terminal phenotypes. Mapping of these lethal mutations shows that sDf2 deletes a minimum of 1.8 map units and a maximum of 2.5 map units. A minimum estimate of essential gene number for the region using a truncated Poisson calculation is 48. The data indicate a minimum estimate of approximately 3500 essential genes in the Caenorhabditis elegans genome.

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baillie D. L., Beckenbach K. A., Rose A. M. Cloning within the unc-43 to unc-31 interval (linkage group IV) of the Caenorhabditis elegans genome using Tc1 linkage selection. Can J Genet Cytol. 1985 Aug;27(4):457–466. doi: 10.1139/g85-067. [DOI] [PubMed] [Google Scholar]
  2. Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974 May;77(1):71–94. doi: 10.1093/genetics/77.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coulson A., Sulston J., Brenner S., Karn J. Toward a physical map of the genome of the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821–7825. doi: 10.1073/pnas.83.20.7821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ferguson E. L., Horvitz H. R. Identification and characterization of 22 genes that affect the vulval cell lineages of the nematode Caenorhabditis elegans. Genetics. 1985 May;110(1):17–72. doi: 10.1093/genetics/110.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gausz J., Hall L. M., Spierer A., Spierer P. Molecular Genetics of the ROSY-ACE Region of DROSOPHILA MELANOGASTER. Genetics. 1986 Jan;112(1):65–78. doi: 10.1093/genetics/112.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greenwald I., Coulson A., Sulston J., Priess J. Correlation of the physical and genetic maps in the lin-12 region of Caenorhabditis elegans. Nucleic Acids Res. 1987 Mar 11;15(5):2295–2307. doi: 10.1093/nar/15.5.2295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Horvitz H. R., Brenner S., Hodgkin J., Herman R. K. A uniform genetic nomenclature for the nematode Caenorhabditis elegans. Mol Gen Genet. 1979 Sep;175(2):129–133. doi: 10.1007/BF00425528. [DOI] [PubMed] [Google Scholar]
  8. Lefevre G., Watkins W. The question of the total gene number in Drosophila melanogaster. Genetics. 1986 Aug;113(4):869–895. doi: 10.1093/genetics/113.4.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Meneely P. M., Herman R. K. Lethals, steriles and deficiencies in a region of the X chromosome of Caenorhabditis elegans. Genetics. 1979 May;92(1):99–115. doi: 10.1093/genetics/92.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Meneely P. M., Herman R. K. Suppression and function of X-linked lethal and sterile mutations in Caenorhabditis elegans. Genetics. 1981 Jan;97(1):65–84. doi: 10.1093/genetics/97.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moerman D. G., Baillie D. L. Formaldehyde mutagenesis in the nematode Caenorhabditis elegans. Mutat Res. 1981 Feb;80(2):273–279. doi: 10.1016/0027-5107(81)90100-7. [DOI] [PubMed] [Google Scholar]
  12. Moerman D. G., Baillie D. L. Genetic Organization in CAENORHABDITIS ELEGANS: Fine-Structure Analysis of the unc-22 Gene. Genetics. 1979 Jan;91(1):95–103. doi: 10.1093/genetics/91.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moerman D. G., Waterston R. H. Spontaneous unstable unc-22 IV mutations in C. elegans var. Bergerac. Genetics. 1984 Dec;108(4):859–877. doi: 10.1093/genetics/108.4.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rogalski T. M., Baillie D. L. Genetic organization of the unc-22 IV gene and the adjacent region in Caenorhabditis elegans. Mol Gen Genet. 1985;201(3):409–414. doi: 10.1007/BF00331331. [DOI] [PubMed] [Google Scholar]
  15. Rogalski T. M., Moerman D. G., Baillie D. L. Essential genes and deficiencies in the unc-22 IV region of Caenorhabditis elegans. Genetics. 1982 Dec;102(4):725–736. doi: 10.1093/genetics/102.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogalski T. M., Riddle D. L. A Caenorhabditis elegans RNA polymerase II gene, ama-1 IV, and nearby essential genes. Genetics. 1988 Jan;118(1):61–74. doi: 10.1093/genetics/118.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rose A. M., Baillie D. L. Genetic organization of the region around UNC-15 (I), a gene affecting paramyosin in Caenorhabditis elegans. Genetics. 1980 Nov;96(3):639–648. doi: 10.1093/genetics/96.3.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rose A. M., Baillie D. L. The Effect of Temperature and Parental Age on Recombination and Nondisjunction in CAENORHABDITIS ELEGANS. Genetics. 1979 Jun;92(2):409–418. doi: 10.1093/genetics/92.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rosenbluth R. E., Baillie D. L. The genetic analysis of a reciprocal translocation, eT1(III; V), in Caenorhabditis elegans. Genetics. 1981 Nov-Dec;99(3-4):415–428. doi: 10.1093/genetics/99.3-4.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES