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. 1988 Jun;119(2):355–363. doi: 10.1093/genetics/119.2.355

Identification of a Putative Structural Gene for Cathepsin D in Caenorhabditis Elegans

L A Jacobson 1, L Jen-Jacobson 1, J M Hawdon 1, G P Owens 1, M A Bolanowski 1, S W Emmons 1, M V Shah 1, R A Pollock 1, D S Conklin 1
PMCID: PMC1203418  PMID: 3396869

Abstract

Mutants of Caenorhabditis elegans having about 10% of wild-type activity of the aspartyl protease cathepsin D have been isolated by screening. Mutant homozygotes have normal growth rates and no obvious morphological or developmental abnormalities. The mutant gene (cad-1) has been mapped to the right extremity of linkage group II. Heterozygous animals (cad-1/+) show intermediate enzyme levels and animals heterozygous for chromosomal deficiencies of the right extremity of linkage group II have 50% of wild-type activity. Cathepsin D purified from a mutant strain has a lower activity per unit mass of pure enzyme. These data suggest that cad-1 is a structural gene for cathepsin D.

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Selected References

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