Skip to main content
Genetics logoLink to Genetics
. 1988 Jul;119(3):549–559. doi: 10.1093/genetics/119.3.549

Allelic and Ectopic Recombination between Ty Elements in Yeast

M Kupiec 1, T D Petes 1
PMCID: PMC1203441  PMID: 2841187

Abstract

Allelic and nonallelic (ectopic) recombination events were analyzed in a set of isogenic strains that carry marked Ty elements. We found that allelic recombination between Ty elements occurred at normal frequencies both in meiosis and mitosis. The marked Ty elements were involved in a large variety of different types of ectopic recombination and this variety was greater in mitosis than in meiosis. Allelic and ectopic recombination events occurred at similar frequencies in mitosis, but allelic recombination predominated in meiosis. Some of the types of ectopic mitotic recombination indicated the common occurrence of concerted recombination events. The length of homology represented by a delta element (330 bp) seemed to be sufficient for some types of mitotic and meiotic recombination.

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boeke J. D., LaCroute F., Fink G. R. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet. 1984;197(2):345–346. doi: 10.1007/BF00330984. [DOI] [PubMed] [Google Scholar]
  2. Botstein D., Falco S. C., Stewart S. E., Brennan M., Scherer S., Stinchcomb D. T., Struhl K., Davis R. W. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene. 1979 Dec;8(1):17–24. doi: 10.1016/0378-1119(79)90004-0. [DOI] [PubMed] [Google Scholar]
  3. Breilmann D., Gafner J., Ciriacy M. Gene conversion and reciprocal exchange in a Ty-mediated translocation in yeast. Curr Genet. 1985;9(7):553–560. doi: 10.1007/BF00381167. [DOI] [PubMed] [Google Scholar]
  4. Casadaban M. J., Martinez-Arias A., Shapira S. K., Chou J. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast. Methods Enzymol. 1983;100:293–308. doi: 10.1016/0076-6879(83)00063-4. [DOI] [PubMed] [Google Scholar]
  5. Chaleff D. T., Fink G. R. Genetic events associated with an insertion mutation in yeast. Cell. 1980 Aug;21(1):227–237. doi: 10.1016/0092-8674(80)90130-0. [DOI] [PubMed] [Google Scholar]
  6. Downs K. M., Brennan G., Liebman S. W. Deletions extending from a single Ty1 element in Saccharomyces cerevisiae. Mol Cell Biol. 1985 Dec;5(12):3451–3457. doi: 10.1128/mcb.5.12.3451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FOGEL S., HURST D. D. Coincidence relations between gene conversion and mitotic recombination in Saccharomyces. Genetics. 1963 Mar;48:321–328. doi: 10.1093/genetics/48.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jinks-Robertson S., Petes T. D. Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics. 1986 Nov;114(3):731–752. doi: 10.1093/genetics/114.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kingsman A. J., Gimlich R. L., Clarke L., Chinault A. C., Carbon J. Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae. J Mol Biol. 1981 Feb 5;145(4):619–632. doi: 10.1016/0022-2836(81)90306-5. [DOI] [PubMed] [Google Scholar]
  10. Klein H. L., Petes T. D. Genetic mapping of Ty elements in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Feb;4(2):329–339. doi: 10.1128/mcb.4.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kohli J., Munz P., Aebi R., Amstutz H., Gysler C., Heyer W. D., Lehmann L., Schuchert P., Szankasi P., Thuriaux P. Interallelic and intergenic conversion in three serine tRNA genes of Schizosaccharomyces pombe. Cold Spring Harb Symp Quant Biol. 1984;49:31–40. doi: 10.1101/sqb.1984.049.01.006. [DOI] [PubMed] [Google Scholar]
  12. Lehrman M. A., Goldstein J. L., Russell D. W., Brown M. S. Duplication of seven exons in LDL receptor gene caused by Alu-Alu recombination in a subject with familial hypercholesterolemia. Cell. 1987 Mar 13;48(5):827–835. doi: 10.1016/0092-8674(87)90079-1. [DOI] [PubMed] [Google Scholar]
  13. Liebman S., Shalit P., Picologlou S. Ty elements are involved in the formation of deletions in DEL1 strains of Saccharomyces cerevisiae. Cell. 1981 Nov;26(3 Pt 1):401–409. doi: 10.1016/0092-8674(81)90209-9. [DOI] [PubMed] [Google Scholar]
  14. Liskay R. M., Stachelek J. L. Evidence for intrachromosomal gene conversion in cultured mouse cells. Cell. 1983 Nov;35(1):157–165. doi: 10.1016/0092-8674(83)90218-0. [DOI] [PubMed] [Google Scholar]
  15. Mikus M. D., Petes T. D. Recombination between genes located on nonhomologous chromosomes in Saccharomyces cerevisiae. Genetics. 1982 Jul-Aug;101(3-4):369–404. doi: 10.1093/genetics/101.3-4.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nicholls R. D., Fischel-Ghodsian N., Higgs D. R. Recombination at the human alpha-globin gene cluster: sequence features and topological constraints. Cell. 1987 May 8;49(3):369–378. doi: 10.1016/0092-8674(87)90289-3. [DOI] [PubMed] [Google Scholar]
  17. Potier S., Winsor B., Lacroute F. Genetic selection for reciprocal translocation at chosen chromosomal sites in Saccharomyces cerevisiae. Mol Cell Biol. 1982 Sep;2(9):1025–1032. doi: 10.1128/mcb.2.9.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Roeder G. S., Fink G. R. DNA rearrangements associated with a transposable element in yeast. Cell. 1980 Aug;21(1):239–249. doi: 10.1016/0092-8674(80)90131-2. [DOI] [PubMed] [Google Scholar]
  19. Roeder G. S., Smith M., Lambie E. J. Intrachromosomal movement of genetically marked Saccharomyces cerevisiae transposons by gene conversion. Mol Cell Biol. 1984 Apr;4(4):703–711. doi: 10.1128/mcb.4.4.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rothstein R. Deletions of a tyrosine tRNA gene in S. cerevisiae. Cell. 1979 May;17(1):185–190. doi: 10.1016/0092-8674(79)90306-4. [DOI] [PubMed] [Google Scholar]
  21. Rothstein R. Double-strand-break repair, gene conversion, and postdivision segregation. Cold Spring Harb Symp Quant Biol. 1984;49:629–637. doi: 10.1101/sqb.1984.049.01.071. [DOI] [PubMed] [Google Scholar]
  22. Rothstein R., Helms C., Rosenberg N. Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Mar;7(3):1198–1207. doi: 10.1128/mcb.7.3.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rouyer F., Simmler M. C., Page D. C., Weissenbach J. A sex chromosome rearrangement in a human XX male caused by Alu-Alu recombination. Cell. 1987 Nov 6;51(3):417–425. doi: 10.1016/0092-8674(87)90637-4. [DOI] [PubMed] [Google Scholar]
  24. Scherer S., Davis R. W. Recombination of dispersed repeated DNA sequences in yeast. Science. 1980 Sep 19;209(4463):1380–1384. doi: 10.1126/science.6251545. [DOI] [PubMed] [Google Scholar]
  25. Warmington J. R., Anwar R., Newlon C. S., Waring R. B., Davies R. W., Indge K. J., Oliver S. G. A 'hot-spot' for Ty transposition on the left arm of yeast chromosome III. Nucleic Acids Res. 1986 Apr 25;14(8):3475–3485. doi: 10.1093/nar/14.8.3475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Warmington J. R., Waring R. B., Newlon C. S., Indge K. J., Oliver S. G. Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast. Nucleic Acids Res. 1985 Sep 25;13(18):6679–6693. doi: 10.1093/nar/13.18.6679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Williamson V. M. Transposable elements in yeast. Int Rev Cytol. 1983;83:1–25. doi: 10.1016/s0074-7696(08)61684-8. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES