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. 1988 Jul;119(3):619–629. doi: 10.1093/genetics/119.3.619

Naturally Occurring Variation in the Restriction Map of the Amy Region of Drosophila Melanogaster

C H Langley 1, A E Shrimpton 1, T Yamazaki 1, N Miyashita 1, Y Matsuo 1, C F Aquadro 1
PMCID: PMC1203447  PMID: 17246437

Abstract

The restriction maps of 85 alleles of the Amy region of Drosophila melanogaster from natural populations were surveyed. A subset of these were also scored for allozyme phenotype and adult enzyme activity of α-amylase. Large insertions were found in 12% of the alleles in a 15-kb region surrounding the two transcriptional units of the duplicated Amy locus. The low frequencies at which each of these large insertions were found are consistent with earlier reports of variation in other loci. Four small deletions were found in the region 5' to the Amy genes. Each was also rare in the population. Restriction site variation provided an estimate of per nucleotide heterozygosity of 0.006. Several statistically significant linkage disequilibria were observed between four polymorphic restriction sites and the allozymes. Adult α-amylase activity was correlated with the allozymes and with the polymorphism at one restriction site close to the transcriptional units.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham I., Doane W. W. Genetic regulation of tissue-specific expression of amylase structural genes in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4446–4450. doi: 10.1073/pnas.75.9.4446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aquadro C. F., Desse S. F., Bland M. M., Langley C. H., Laurie-Ahlberg C. C. Molecular population genetics of the alcohol dehydrogenase gene region of Drosophila melanogaster. Genetics. 1986 Dec;114(4):1165–1190. doi: 10.1093/genetics/114.4.1165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barrie P. A., Jeffreys A. J., Scott A. F. Evolution of the beta-globin gene cluster in man and the primates. J Mol Biol. 1981 Jul 5;149(3):319–336. doi: 10.1016/0022-2836(81)90476-9. [DOI] [PubMed] [Google Scholar]
  4. Benkel B. F., Abukashawa S., Boer P. H., Hickey D. A. Molecular cloning of DNA complementary to Drosophila melanogaster alpha-amylase mRNA. Genome. 1987 Jun;29(3):510–515. doi: 10.1139/g87-087. [DOI] [PubMed] [Google Scholar]
  5. Benkel B. F., Hickey D. A. The Interaction of Genetic and Environmental Factors in the Control of Amylase Gene Expression in DROSOPHILA MELANOGASTER. Genetics. 1986 Nov;114(3):943–954. doi: 10.1093/genetics/114.3.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Boer P. H., Hickey D. A. The alpha-amylase gene in Drosophila melanogaster: nucleotide sequence, gene structure and expression motifs. Nucleic Acids Res. 1986 Nov 11;14(21):8399–8411. doi: 10.1093/nar/14.21.8399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brown A. J. Variation at the 87A heat shock locus in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5350–5354. doi: 10.1073/pnas.80.17.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cross S. R., Birley A. J. Restriction endonuclease map variation in the Adh region in populations of Drosophila melanogaster. Biochem Genet. 1986 Jun;24(5-6):415–433. doi: 10.1007/BF00499097. [DOI] [PubMed] [Google Scholar]
  9. Davis P. S., Shen M. W., Judd B. H. Asymmetrical pairings of transposons in and proximal to the white locus of Drosophila account for four classes of regularly occurring exchange products. Proc Natl Acad Sci U S A. 1987 Jan;84(1):174–178. doi: 10.1073/pnas.84.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Doane W. W., Gemmill R. M., Schwartz P. E., Hawley S. A., Norman R. A. Structural organization of the alpha-amylase gene locus in Drosophila melanogaster and Drosophila miranda. Isozymes Curr Top Biol Med Res. 1987;14:229–266. [PubMed] [Google Scholar]
  11. Ewens W. J., Spielman R. S., Harris H. Estimation of genetic variation at the DNA level from restriction endonuclease data. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3748–3750. doi: 10.1073/pnas.78.6.3748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Finnegan D. J. Transposable elements in eukaryotes. Int Rev Cytol. 1985;93:281–326. doi: 10.1016/s0074-7696(08)61376-5. [DOI] [PubMed] [Google Scholar]
  13. Gemmill R. M., Levy J. N., Doane W. W. Molecular cloning of alpha-amylase genes from Drosophila melanogaster. I. Clone isolation by use of a mouse probe. Genetics. 1985 Jun;110(2):299–312. doi: 10.1093/genetics/110.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gemmill R. M., Schwartz P. E., Doane W. W. Structural organization of the Amy locus in seven strains of Drosophila melanogaster. Nucleic Acids Res. 1986 Jul 11;14(13):5337–5352. doi: 10.1093/nar/14.13.5337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Goldberg M. L., Sheen J. Y., Gehring W. J., Green M. M. Unequal crossing-over associated with asymmetrical synapsis between nomadic elements in the Drosophila melanogaster genome. Proc Natl Acad Sci U S A. 1983 Aug;80(16):5017–5021. doi: 10.1073/pnas.80.16.5017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Golding G. B., Aquadro C. F., Langley C. H. Sequence evolution within populations under multiple types of mutation. Proc Natl Acad Sci U S A. 1986 Jan;83(2):427–431. doi: 10.1073/pnas.83.2.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Green M M. Non-Homologous Pairing and Crossing over in Drosophila Melanogaster. Genetics. 1959 Nov;44(6):1243–1256. doi: 10.1093/genetics/44.6.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hazelrigg T., Levis R., Rubin G. M. Transformation of white locus DNA in drosophila: dosage compensation, zeste interaction, and position effects. Cell. 1984 Feb;36(2):469–481. doi: 10.1016/0092-8674(84)90240-x. [DOI] [PubMed] [Google Scholar]
  19. Hudson R. R. Estimating genetic variability with restriction endonucleases. Genetics. 1982 Apr;100(4):711–719. doi: 10.1093/genetics/100.4.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Jinks-Robertson S., Petes T. D. Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics. 1986 Nov;114(3):731–752. doi: 10.1093/genetics/114.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Judd B H. Studies on Some Position Pseudoalleles at the White Region in Drosophila Melanogaster. Genetics. 1959 Jan;44(1):34–42. doi: 10.1093/genetics/44.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Klarenberg A. J., Scharloo W. Nonrandom Association between Structural Amy and Regulatory map Variants in DROSOPHILA MELANOGASTER. Genetics. 1986 Nov;114(3):875–884. doi: 10.1093/genetics/114.3.875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kreitman M. E., Aguadé M. Excess polymorphism at the Adh locus in Drosophila melanogaster. Genetics. 1986 Sep;114(1):93–110. doi: 10.1093/genetics/114.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  25. Langley C. H., Aquadro C. F. Restriction-map variation in natural populations of Drosophila melanogaster: white-locus region. Mol Biol Evol. 1987 Nov;4(6):651–663. doi: 10.1093/oxfordjournals.molbev.a040467. [DOI] [PubMed] [Google Scholar]
  26. Langley C. H., Ito K., Voelker R. A. Linkage disequilibrium in natural populations of Drosophila melanogaster Seasonal variation. Genetics. 1977 Jun;86(2 Pt 1):447–454. [PMC free article] [PubMed] [Google Scholar]
  27. Langley C. H., Montgomery E., Quattlebaum W. F. Restriction map variation in the Adh region of Drosophila. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5631–5635. doi: 10.1073/pnas.79.18.5631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Langley C. H., Tobari Y. N., Kojima K. I. Linkage disequilibrium in natural populations of Drosophila melanogaster. Genetics. 1974 Nov;78(3):921–936. doi: 10.1093/genetics/78.3.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Langley C. H., Voelker R. A., Brown A. J., Ohnishi S., Dickson B., Montgomery E. Null allele frequencies at allozyme loci in natural populations of Drosophila melanogaster. Genetics. 1981 Sep;99(1):151–156. doi: 10.1093/genetics/99.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Laurie-Ahlberg C. C., Maroni G., Bewley G. C., Lucchesi J. C., Weir B. S. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073–1077. doi: 10.1073/pnas.77.2.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Laurie-Ahlberg C. C., Stam L. F. Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster. Genetics. 1987 Jan;115(1):129–140. doi: 10.1093/genetics/115.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Levy J. N., Gemmill R. M., Doane W. W. Molecular cloning of alpha-amylase genes from Drosophila melanogaster. II. Clone organization and verification. Genetics. 1985 Jun;110(2):313–324. doi: 10.1093/genetics/110.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Montgomery E. A., Langley C. H. Transposable Elements in Mendelian Populations. II. Distribution of Three COPIA-like Elements in a Natural Population of DROSOPHILA MELANOGASTER. Genetics. 1983 Jul;104(3):473–483. doi: 10.1093/genetics/104.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Montgomery E., Charlesworth B., Langley C. H. A test for the role of natural selection in the stabilization of transposable element copy number in a population of Drosophila melanogaster. Genet Res. 1987 Feb;49(1):31–41. doi: 10.1017/s0016672300026707. [DOI] [PubMed] [Google Scholar]
  35. Muki T., Watanabe T. K., Yamaguchi O. The genetic structure of natural populations of Drosophila melanogaster. XII. Linkage disequilibrium in a large local population. Genetics. 1974 Aug;77(4):771–793. doi: 10.1093/genetics/77.4.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Nei M., Li W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269–5273. doi: 10.1073/pnas.76.10.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Nei M., Tajima F. DNA polymorphism detectable by restriction endonucleases. Genetics. 1981 Jan;97(1):145–163. doi: 10.1093/genetics/97.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Schaeffer S. W., Aquadro C. F., Langley C. H. Restriction-map variation in the Notch region of Drosophila melanogaster. Mol Biol Evol. 1988 Jan;5(1):30–40. doi: 10.1093/oxfordjournals.molbev.a040475. [DOI] [PubMed] [Google Scholar]
  39. Singh R. S., Coulthart M. B. Genic variation in abundant soluble proteins of Drosophila melanogaster and Drosophila pseudoobscura. Genetics. 1982 Nov;102(3):437–453. doi: 10.1093/genetics/102.3.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Singh R. S., Hickey D. A., David J. Genetic Differentiation between Geographically Distant Populations of DROSOPHILA MELANOGASTER. Genetics. 1982 Jun;101(2):235–256. doi: 10.1093/genetics/101.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Voelker R. A., Langley C. H., Brown A. J., Ohnishi S., Dickson B., Montgomery E., Smith S. C. Enzyme null alleles in natural populations of Drosophila melanogaster: Frequencies in a North Carolina population. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1091–1095. doi: 10.1073/pnas.77.2.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Yamazaki T., Matsuo Y. Genetic Analysis of Natural Populations of DROSOPHILA MELANOGASTER in Japan. III. Genetic Variability of Inducing Factors of Amylase and Fitness. Genetics. 1984 Sep;108(1):223–235. doi: 10.1093/genetics/108.1.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. de Jong G., Hoorn A. J., Thörig G. E., Scharloo W. Frequencies of amylase variants in Drosophila melanogaster. Nature. 1972 Aug 25;238(5365):453–454. doi: 10.1038/238453a0. [DOI] [PubMed] [Google Scholar]

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