Abstract
A cut was made on the middorsal skin of newborn mice of strains C57BL/10J, C57BL/10J-A/A, and C3H/He using fine iridectomy scissors. In the epidermis within 1 mm of the wound edge in C57BL/10J and C57BL/10J-A/A, the melanocyte population positive to the dopa reaction as well as the melanoblast-melanocyte population positive to the combined dopa-premelanin reaction increased dramatically until the 3rd day, then gradually decreased. In contrast, the melanocyte population of C3H/He did not increase after wounding, despite that the melanoblast-melanocyte population increased. Pigment-producing melanocytes in mitosis were frequently found in C57BL/10J and C57BL/10J-A/A, but not in C3H/He. The F(1), F(2), and backcross matings were performed to get some information about the genetic basis of the difference between C57BL/10J and C3H/He. In the F(1) generation the offspring from reciprocal crosses exhibited intermediate values in both populations on the 3rd day after wounding. The F(2) generation included the C3H/He type, F(1) type, and C57BL/10J type in a ratio of 1:2:1 in both populations. Moreover, both reciprocal backcrosses gave 1:1 ratios of parent type to F(1) type in both populations. These results indicate that the proliferative activity of mouse epidermal melanocytes during the healing of skin wounds are controlled by semidominant genes.
Full Text
The Full Text of this article is available as a PDF (2.7 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hirobe T. Genes involved in regulating the melanocyte and melanoblast-melanocyte populations in the epidermis of newborn mouse skin. J Exp Zool. 1982 Nov 1;223(3):257–264. doi: 10.1002/jez.1402230307. [DOI] [PubMed] [Google Scholar]
- Hirobe T. Histochemical survey of the distribution of the epidermal melanoblasts and melanocytes in the mouse during fetal and postnatal periods. Anat Rec. 1984 Apr;208(4):589–594. doi: 10.1002/ar.1092080414. [DOI] [PubMed] [Google Scholar]
- Jimbow K., Roth S. I., Fitzpatrick T. B., Szabo G. Mitotic activity in non-neoplastic melanocytes in vivo as determined by histochemical, autoradiographic, and electron microscope studies. J Cell Biol. 1975 Sep;66(3):663–670. doi: 10.1083/jcb.66.3.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MISHIMA Y. ELECTRON MICROSCOPIC CYTOCHEMISTRY OF MELANOSOMES AND MITOCHONDRIA. J Histochem Cytochem. 1964 Oct;12:784–790. doi: 10.1177/12.10.784. [DOI] [PubMed] [Google Scholar]
- MISHIMA Y. New technic for comprehensive demonstration of melanin, premelanin, and tyrosinase sites. Combined dopapremelanin reaction. J Invest Dermatol. 1960 Jun;34:355–360. doi: 10.1038/jid.1960.62. [DOI] [PubMed] [Google Scholar]
- Mayer T. C. The migratory pathway of neural crest cells into the skin of mouse embryos. Dev Biol. 1973 Sep;34(1):39–46. doi: 10.1016/0012-1606(73)90337-0. [DOI] [PubMed] [Google Scholar]
- Rosdahl I. K., Lindström S. Morphology of epidermal melanocytes in different stages of mitosis. Acta Derm Venereol. 1980;60(3):209–215. [PubMed] [Google Scholar]
- Rosdahl I. K. Melanocyte mitosis in UVB-irradiated mouse skin. Acta Derm Venereol. 1978;58(3):217–221. [PubMed] [Google Scholar]
- Rosdahl I. K., Szabo G. Thymidine labelling of epidermal melanocytes in UV-irradiated skin. Acta Derm Venereol. 1976;56(2):159–161. [PubMed] [Google Scholar]
- Rosdahl I. K., Szabó G. Mitotic activity of epidermal melanocytes in UV-irradiated mouse skin. J Invest Dermatol. 1978 Mar;70(3):143–148. doi: 10.1111/1523-1747.ep12258559. [DOI] [PubMed] [Google Scholar]
- Rosdahl I., Bagge U. Vital microscopy of epidermal melanocytes. Acta Derm Venereol. 1981;61(1):55–58. [PubMed] [Google Scholar]