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. 1989 Feb;121(2):333–352. doi: 10.1093/genetics/121.2.333

Zygotic Lethals with Specific Maternal Effect Phenotypes in Drosophila Melanogaster. I. Loci on the X Chromosome

N Perrimon 1, L Engstrom 1, A P Mahowald 1
PMCID: PMC1203621  PMID: 2499512

Abstract

In order to identify all X-linked zygotic lethal loci that exhibit a specific maternal effect on embryonic development, germline clonal analyses of X-linked zygotic lethal mutations have been performed. Two strategies were employed. In Screen A germline clonal analysis of 441 mutations at 211 previously mapped X-linked loci within defined regions was performed. In Screen B germline clonal analysis of 581 larval and pupal mutations distributed throughout the entire length of the X chromosome was performed. These approaches provide an 86% level of saturation for X-linked late zygotic lethals (larval and pupal) with specific maternal effect embryonic lethal phenotypes. The maternal effect phenotypes of these mutations are described.

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Selected References

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  1. Akam M. The molecular basis for metameric pattern in the Drosophila embryo. Development. 1987 Sep;101(1):1–22. [PubMed] [Google Scholar]
  2. Baker N. E. Molecular cloning of sequences from wingless, a segment polarity gene in Drosophila: the spatial distribution of a transcript in embryos. EMBO J. 1987 Jun;6(6):1765–1773. doi: 10.1002/j.1460-2075.1987.tb02429.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Busson D., Gans M., Komitopoulou K., Masson M. Genetic Analysis of Three Dominant Female-Sterile Mutations Located on the X Chromosome of DROSOPHILA MELANOGASTER. Genetics. 1983 Oct;105(2):309–325. doi: 10.1093/genetics/105.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cabrera C. V., Alonso M. C., Johnston P., Phillips R. G., Lawrence P. A. Phenocopies induced with antisense RNA identify the wingless gene. Cell. 1987 Aug 14;50(4):659–663. doi: 10.1016/0092-8674(87)90039-0. [DOI] [PubMed] [Google Scholar]
  5. Degelmann A., Hardy P. A., Perrimon N., Mahowald A. P. Developmental analysis of the torso-like phenotype in Drosophila produced by a maternal-effect locus. Dev Biol. 1986 Jun;115(2):479–489. doi: 10.1016/0012-1606(86)90268-x. [DOI] [PubMed] [Google Scholar]
  6. Dietrich U., Campos-Ortega J. A. The expression of neurogenic loci in imaginal epidermal cells of Drosophila melanogaster. J Neurogenet. 1984 Dec;1(4):315–332. doi: 10.3109/01677068409107094. [DOI] [PubMed] [Google Scholar]
  7. Doe C. Q., Scott M. P. Segmentation and homeotic gene function in the developing nervous system of Drosophila. Trends Neurosci. 1988 Mar;11(3):101–106. doi: 10.1016/0166-2236(88)90154-3. [DOI] [PubMed] [Google Scholar]
  8. Gans M., Audit C., Masson M. Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster. Genetics. 1975 Dec;81(4):683–704. doi: 10.1093/genetics/81.4.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Garcia-Bellido A., Robbins L. G. Viability of Female Germ-Line Cells Homozygous for Zygotic Lethals in DROSOPHILA MELANOGASTER. Genetics. 1983 Feb;103(2):235–247. doi: 10.1093/genetics/103.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gerttula S., Jin Y. S., Anderson K. V. Zygotic expression and activity of the Drosophila Toll gene, a gene required maternally for embryonic dorsal-ventral pattern formation. Genetics. 1988 May;119(1):123–133. doi: 10.1093/genetics/119.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Haenlin M., Steller H., Pirrotta V., Mohier E. A 43 kilobase cosmid P transposon rescues the fs(1)K10 morphogenetic locus and three adjacent Drosophila developmental mutants. Cell. 1985 Apr;40(4):827–837. doi: 10.1016/0092-8674(85)90342-3. [DOI] [PubMed] [Google Scholar]
  12. Komitopoulou K., Gans M., Margaritis L. H., Kafatos F. C., Masson M. Isolation and Characterization of Sex-Linked Female-Sterile Mutants in DROSOPHILA MELANOGASTER with Special Attention to Eggshell Mutants. Genetics. 1983 Dec;105(4):897–920. doi: 10.1093/genetics/105.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Konrad K. D., Engstrom L., Perrimon N., Mahowald A. P. Genetic analysis of oogenesis and the role of maternal gene expression in early development. Dev Biol (N Y 1985) 1985;1:577–617. doi: 10.1007/978-1-4615-6814-8_13. [DOI] [PubMed] [Google Scholar]
  14. LaBonne S. G., Mahowald A. P. Partial rescue of embryos from two maternal-effect neurogenic mutants by transplantation of wild-type ooplasm. Dev Biol. 1985 Jul;110(1):264–267. doi: 10.1016/0012-1606(85)90084-3. [DOI] [PubMed] [Google Scholar]
  15. Lefevre G., Watkins W. The question of the total gene number in Drosophila melanogaster. Genetics. 1986 Aug;113(4):869–895. doi: 10.1093/genetics/113.4.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MacKrell A. J., Blumberg B., Haynes S. R., Fessler J. H. The lethal myospheroid gene of Drosophila encodes a membrane protein homologous to vertebrate integrin beta subunits. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2633–2637. doi: 10.1073/pnas.85.8.2633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mohler J. D. Developmental genetics of the Drosophila egg. I. Identification of 59 sex-linked cistrons with maternal effects on embryonic development. Genetics. 1977 Feb;85(2):259–272. doi: 10.1093/genetics/85.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mortin M. A., Perrimon N., Bonner J. J. Clonal analysis of two mutations in the large subunit of RNA polymerase II of Drosophila. Mol Gen Genet. 1985;199(3):421–426. doi: 10.1007/BF00330753. [DOI] [PubMed] [Google Scholar]
  19. Nüsslein-Volhard C., Frohnhöfer H. G., Lehmann R. Determination of anteroposterior polarity in Drosophila. Science. 1987 Dec 18;238(4834):1675–1681. doi: 10.1126/science.3686007. [DOI] [PubMed] [Google Scholar]
  20. Papkoff J., Brown A. M., Varmus H. E. The int-1 proto-oncogene products are glycoproteins that appear to enter the secretory pathway. Mol Cell Biol. 1987 Nov;7(11):3978–3984. doi: 10.1128/mcb.7.11.3978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Perrimon N. Clonal Analysis of Dominant Female-Sterile, Germline-Dependent Mutations in DROSOPHILA MELANOGASTER. Genetics. 1984 Dec;108(4):927–939. doi: 10.1093/genetics/108.4.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Perrimon N., Engstrom L., Mahowald A. P. Developmental genetics of the 2C-D region of the Drosophila X chromosome. Genetics. 1985 Sep;111(1):23–41. doi: 10.1093/genetics/111.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Perrimon N., Engstrom L., Mahowald A. P. The effects of zygotic lethal mutations on female germ-line functions in Drosophila. Dev Biol. 1984 Oct;105(2):404–414. doi: 10.1016/0012-1606(84)90297-5. [DOI] [PubMed] [Google Scholar]
  24. Perrimon N., Gans M. Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237. Dev Biol. 1983 Dec;100(2):365–373. doi: 10.1016/0012-1606(83)90231-2. [DOI] [PubMed] [Google Scholar]
  25. Perrimon N., Mahowald A. P. Multiple functions of segment polarity genes in Drosophila. Dev Biol. 1987 Feb;119(2):587–600. doi: 10.1016/0012-1606(87)90061-3. [DOI] [PubMed] [Google Scholar]
  26. Perrimon N., Mohler D., Engstrom L., Mahowald A. P. X-linked female-sterile loci in Drosophila melanogaster. Genetics. 1986 Jul;113(3):695–712. doi: 10.1093/genetics/113.3.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Perrimon N., Smouse D., Miklos G. L. Developmental genetics of loci at the base of the X chromosome of Drosophila melanogaster. Genetics. 1989 Feb;121(2):313–331. doi: 10.1093/genetics/121.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rijsewijk F., Schuermann M., Wagenaar E., Parren P., Weigel D., Nusse R. The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell. 1987 Aug 14;50(4):649–657. doi: 10.1016/0092-8674(87)90038-9. [DOI] [PubMed] [Google Scholar]
  29. Shannon M. P., Kaufman T. C., Shen M. W., Judd B. H. Lethality patterns and morphology of selected lethal and semi-lethal mutations in the zeste-white region of Drosophila melanogaster. Genetics. 1972 Dec;72(4):615–638. doi: 10.1093/genetics/72.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Simpson P. Maternal-Zygotic Gene Interactions during Formation of the Dorsoventral Pattern in Drosophila Embryos. Genetics. 1983 Nov;105(3):615–632. doi: 10.1093/genetics/105.3.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wright T. R. The genetics of embryogenesis in Drosophila. Adv Genet. 1970;15:261–395. doi: 10.1016/s0065-2660(08)60075-9. [DOI] [PubMed] [Google Scholar]

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