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. 1989 May;122(1):65–71. doi: 10.1093/genetics/122.1.65

A Large Cluster of Highly Expressed Genes Is Dispensable for Growth and Development in Aspergillus Nidulans

R Aramayo 1, T H Adams 1, W E Timberlake 1
PMCID: PMC1203693  PMID: 2471671

Abstract

We investigated the functions of the highly expressed, sporulation-specific SpoC1 genes of Aspergillus nidulans by deleting the entire 38-kb SpoC1 gene cluster. The resultant mutant strain did not differ from the wild type in (1) growth rate, (2) morphology of specialized reproductive structures formed during completion of the asexual or sexual life cycles, (3) sporulation efficiency, (4) spore viability or (5) spore resistance to environmental stress. Thus, deletion of the SpoC1 gene cluster, representing 0.15% of the A. nidulans genome, had no readily detectable phenotypic effects. Implications of this result are discussed in the context of major alterations in gene expression that occur during A. nidulans development.

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Selected References

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  1. Adams T. H., Boylan M. T., Timberlake W. E. brlA is necessary and sufficient to direct conidiophore development in Aspergillus nidulans. Cell. 1988 Jul 29;54(3):353–362. doi: 10.1016/0092-8674(88)90198-5. [DOI] [PubMed] [Google Scholar]
  2. Bainbridge B. W. Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans. J Gen Microbiol. 1971 Jun;66(3):319–325. doi: 10.1099/00221287-66-3-319. [DOI] [PubMed] [Google Scholar]
  3. Beever R. E., Laracy E. P. Osmotic adjustment in the filamentous fungus Aspergillus nidulans. J Bacteriol. 1986 Dec;168(3):1358–1365. doi: 10.1128/jb.168.3.1358-1365.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berse B., Dmochowska A., Skrzypek M., Wegleński P., Bates M. A., Weiss R. L. Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans. Gene. 1983 Nov;25(1):109–117. doi: 10.1016/0378-1119(83)90173-7. [DOI] [PubMed] [Google Scholar]
  5. Caddick M. X., Arst H. N., Jr, Taylor L. H., Johnson R. I., Brownlee A. G. Cloning of the regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. EMBO J. 1986 May;5(5):1087–1090. doi: 10.1002/j.1460-2075.1986.tb04326.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Caddick M. X., Brownlee A. G., Arst H. N., Jr Regulation of gene expression by pH of the growth medium in Aspergillus nidulans. Mol Gen Genet. 1986 May;203(2):346–353. doi: 10.1007/BF00333978. [DOI] [PubMed] [Google Scholar]
  7. Garber A. T., Segall J. The SPS4 gene of Saccharomyces cerevisiae encodes a major sporulation-specific mRNA. Mol Cell Biol. 1986 Dec;6(12):4478–4485. doi: 10.1128/mcb.6.12.4478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gwynne D. I., Miller B. L., Miller K. Y., Timberlake W. E. Structure and regulated expression of the SpoC1 gene cluster from Aspergillus nidulans. J Mol Biol. 1984 Nov 25;180(1):91–109. doi: 10.1016/0022-2836(84)90432-7. [DOI] [PubMed] [Google Scholar]
  9. Hawkins A. R., Francisco Da Silva A. J., Roberts C. F. Cloning and characterization of the three enzyme structural genes QUTB, QUTC and QUTE from the quinic acid utilization gene cluster in Aspergillus nidulans. Curr Genet. 1985;9(4):305–311. doi: 10.1007/BF00419960. [DOI] [PubMed] [Google Scholar]
  10. Hawkins A. R., Giles N. H., Kinghorn J. R. Genetical and biochemical aspects of quinate breakdown in the filamentous fungus Aspergillus nidulans. Biochem Genet. 1982 Apr;20(3-4):271–286. doi: 10.1007/BF00484424. [DOI] [PubMed] [Google Scholar]
  11. Kohara Y., Akiyama K., Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. doi: 10.1016/0092-8674(87)90503-4. [DOI] [PubMed] [Google Scholar]
  12. Kuttin E. S., Kaplan W., Scholer H. I., Burtscher H., Köhler H. Sexual and asexual reproduction of Aspergillus nidulans in vivo. Mykosen. 1985 Mar;28(3):109–116. doi: 10.1111/j.1439-0507.1985.tb02103.x. [DOI] [PubMed] [Google Scholar]
  13. Lindquist S. The heat-shock response. Annu Rev Biochem. 1986;55:1151–1191. doi: 10.1146/annurev.bi.55.070186.005443. [DOI] [PubMed] [Google Scholar]
  14. Macdonald K. D., Holt G. Genetics of biosynthesis and overproduction of penicillin. Sci Prog. 1976 Winter;63(252):547–573. [PubMed] [Google Scholar]
  15. Makins J. F., Holt G., Macdonald K. D. The genetic location of three mutations impairing penicillin production in Aspergillus nidulans. J Gen Microbiol. 1983 Oct;129(10):3027–3033. doi: 10.1099/00221287-129-10-3027. [DOI] [PubMed] [Google Scholar]
  16. Marsh J. L., Erfle M., Wykes E. J. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene. 1984 Dec;32(3):481–485. doi: 10.1016/0378-1119(84)90022-2. [DOI] [PubMed] [Google Scholar]
  17. May G. S., Tsang M. L., Smith H., Fidel S., Morris N. R. Aspergillus nidulans beta-tubulin genes are unusually divergent. Gene. 1987;55(2-3):231–243. doi: 10.1016/0378-1119(87)90283-6. [DOI] [PubMed] [Google Scholar]
  18. Miller B. L., Miller K. Y., Roberti K. A., Timberlake W. E. Position-dependent and -independent mechanisms regulate cell-specific expression of the SpoC1 gene cluster of Aspergillus nidulans. Mol Cell Biol. 1987 Jan;7(1):427–434. doi: 10.1128/mcb.7.1.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Miller B. L., Miller K. Y., Timberlake W. E. Direct and indirect gene replacements in Aspergillus nidulans. Mol Cell Biol. 1985 Jul;5(7):1714–1721. doi: 10.1128/mcb.5.7.1714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mullaney E. J., Hamer J. E., Roberti K. A., Yelton M. M., Timberlake W. E. Primary structure of the trpC gene from Aspergillus nidulans. Mol Gen Genet. 1985;199(1):37–45. doi: 10.1007/BF00327506. [DOI] [PubMed] [Google Scholar]
  21. Orr W. C., Timberlake W. E. Clustering of spore-specific genes in Aspergillus nidulans. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5976–5980. doi: 10.1073/pnas.79.19.5976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. PONTECORVO G., ROPER J. A., HEMMONS L. M., MACDONALD K. D., BUFTON A. W. J. The genetics of Aspergillus nidulans. Adv Genet. 1953;5:141–238. doi: 10.1016/s0065-2660(08)60408-3. [DOI] [PubMed] [Google Scholar]
  23. Parker R., Simmons T., Shuster E. O., Siliciano P. G., Guthrie C. Genetic analysis of small nuclear RNAs in Saccharomyces cerevisiae: viable sextuple mutant. Mol Cell Biol. 1988 Aug;8(8):3150–3159. doi: 10.1128/mcb.8.8.3150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Percival-Smith A., Segall J. Characterization and mutational analysis of a cluster of three genes expressed preferentially during sporulation of Saccharomyces cerevisiae. Mol Cell Biol. 1986 Jul;6(7):2443–2451. doi: 10.1128/mcb.6.7.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Petko L., Lindquist S. Hsp26 is not required for growth at high temperatures, nor for thermotolerance, spore development, or germination. Cell. 1986 Jun 20;45(6):885–894. doi: 10.1016/0092-8674(86)90563-5. [DOI] [PubMed] [Google Scholar]
  26. Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]
  27. Timberlake W. E., Barnard E. C. Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans. Cell. 1981 Oct;26(1 Pt 1):29–37. doi: 10.1016/0092-8674(81)90030-1. [DOI] [PubMed] [Google Scholar]
  28. Timberlake W. E. Developmental gene regulation in Aspergillus nidulans. Dev Biol. 1980 Aug;78(2):497–510. doi: 10.1016/0012-1606(80)90349-8. [DOI] [PubMed] [Google Scholar]
  29. Timberlake W. E., Marshall M. A. Genetic regulation of development in Aspergillus nidulans. Trends Genet. 1988 Jun;4(6):162–169. doi: 10.1016/0168-9525(88)90022-4. [DOI] [PubMed] [Google Scholar]
  30. Upshall A., Gilbert T., Saari G., O'Hara P. J., Weglenski P., Berse B., Miller K., Timberlake W. E. Molecular analysis of the argB gene of Aspergillus nidulans. Mol Gen Genet. 1986 Aug;204(2):349–354. doi: 10.1007/BF00425521. [DOI] [PubMed] [Google Scholar]
  31. Yager L. N., Kurtz M. B., Champe S. P. Temperature-shift analysis of conidial development in Aspergillus nidulans. Dev Biol. 1982 Sep;93(1):92–103. doi: 10.1016/0012-1606(82)90242-1. [DOI] [PubMed] [Google Scholar]
  32. Yamashita I., Fukui S. Transcriptional control of the sporulation-specific glucoamylase gene in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1985 Nov;5(11):3069–3073. doi: 10.1128/mcb.5.11.3069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yelton M. M., Hamer J. E., Timberlake W. E. Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1470–1474. doi: 10.1073/pnas.81.5.1470. [DOI] [PMC free article] [PubMed] [Google Scholar]

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