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. 1989 Jun;122(2):331–339. doi: 10.1093/genetics/122.2.331

Characterization of the Amdr-Controlled Lama and Lamb Genes of Aspergillus Nidulans

M E Katz 1, M J Hynes 1
PMCID: PMC1203705  PMID: 2670667

Abstract

Four Aspergillus nidulans genes are known to be under the control of the trans-acting regulatory gene amdR. We describe the isolation and initial characterization of one of these amdR-regulated genes, lamA. The lam locus, however, was found to consist of two divergently transcribed genes, the lamA gene, and a new gene, also under amdR control, which we have designated lamB. Using recombinant DNA techniques we have constructed a strain of A. nidulans lacking a functional lamB gene. Experiments conducted with this strain demonstrate that lamB, like lamA, is involved in utilization of 2-pyrrolidinone in A. nidulans. Metabolism of a related compound, γ-amino butyric acid (GABA) is not affected. We also provide evidence that the conversion of exogenous 2-pyrrolidinone to endogenous GABA requires a functional lamB gene. The expression of both lamA and lamB is subject to carbon and nitrogen metabolite repression in addition to amdR-mediated induction by ω-amino acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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