Abstract
A new experimental system for inducing mitochondrial DNA heteroplasmy in Drosophila was developed. By transplanting the germ plasm of Drosophila melanogaster and Drosophila mauritiana into the posterior pole of the recipient eggs of D. melanogaster, it was possible to introduce foreign mitochondria into the recipient female germline. Heteroplasmic individuals containing both donor and recipient mtDNA were obtained in intra- and interspecific combinations at similar frequencies. The proportion of donor-derived mtDNA in the heteroplasmic individuals varied considerably from individual to individual irrespective of the donor species used. No significant decrease in or elimination of donor mtDNA was observed, and the heteroplasmic state in female germlines persisted for several generations. The present system should serve very much to promote the study and clarification of the transmission genetics of mtDNA in insects.
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Selected References
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- De Francesco L., Attardi G., Croce C. M. Uniparental propagation of mitochondrial DNA in mouse-human cell hybrids. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4079–4083. doi: 10.1073/pnas.77.7.4079. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox T. D., Sanford J. C., McMullin T. W. Plasmids can stably transform yeast mitochondria lacking endogenous mtDNA. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7288–7292. doi: 10.1073/pnas.85.19.7288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harrison R. G., Rand D. M., Wheeler W. C. Mitochondrial DNA size variation within individual crickets. Science. 1985 Jun 21;228(4706):1446–1448. doi: 10.1126/science.228.4706.1446. [DOI] [PubMed] [Google Scholar]
- Hauswirth W. W., Clayton D. A. Length heterogeneity of a conserved displacement-loop sequence in human mitochondrial DNA. Nucleic Acids Res. 1985 Nov 25;13(22):8093–8104. doi: 10.1093/nar/13.22.8093. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hauswirth W. W., Laipis P. J. Mitochondrial DNA polymorphism in a maternal lineage of Holstein cows. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4686–4690. doi: 10.1073/pnas.79.15.4686. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Illmensee K., Mahowald A. P. Transplantation of posterior polar plasm in Drosophila. Induction of germ cells at the anterior pole of the egg. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1016–1020. doi: 10.1073/pnas.71.4.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnston S. A., Anziano P. Q., Shark K., Sanford J. C., Butow R. A. Mitochondrial transformation in yeast by bombardment with microprojectiles. Science. 1988 Jun 10;240(4858):1538–1541. doi: 10.1126/science.2836954. [DOI] [PubMed] [Google Scholar]
- King M. P., Attardi G. Injection of mitochondria into human cells leads to a rapid replacement of the endogenous mitochondrial DNA. Cell. 1988 Mar 25;52(6):811–819. doi: 10.1016/0092-8674(88)90423-0. [DOI] [PubMed] [Google Scholar]
- Mahowald A. P., Illmensee K., Turner F. R. Interspecific transplantation of polar plasm between Drosophila embryos. J Cell Biol. 1976 Aug;70(2 Pt 1):358–373. doi: 10.1083/jcb.70.2.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rand D. M., Harrison R. G. Mitochondrial DNA transmission genetics in crickets. Genetics. 1986 Nov;114(3):955–970. doi: 10.1093/genetics/114.3.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Solignac M., Monnerot M., Mounolou J. C. Mitochondrial DNA heteroplasmy in Drosophila mauritiana. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6942–6946. doi: 10.1073/pnas.80.22.6942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White F. A., Bunn C. L. Segregation of mitochondrial DNA in human somatic cell hybrids. Mol Gen Genet. 1984;197(3):453–460. doi: 10.1007/BF00329942. [DOI] [PubMed] [Google Scholar]