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. 1989 Aug;122(4):759–772. doi: 10.1093/genetics/122.4.759

Mitotic and Meiotic Gene Conversion of Ty Elements and Other Insertions in Saccharomyces Cerevisiae

A Vincent 1, T D Petes 1
PMCID: PMC1203752  PMID: 2547693

Abstract

We examined meiotic and mitotic gene conversion events involved in deletion of Ty elements and other insertions from the genome of the yeast Saccharomyces cerevisiae. We found that Ty elements and one other insertion were deleted by mitotic gene conversion less frequently than point mutations at the same loci. One non-Ty insertion similar in size to Ty, however, did not show this bias. Mitotic conversion events deleting insertions were more frequently associated with crossing over than those deleting point mutations. In meiosis, conversion events duplicating the element were more common than those that deleted the element for one of the loci (HIS4) examined.

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Selected References

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  1. Boeke J. D., Garfinkel D. J., Styles C. A., Fink G. R. Ty elements transpose through an RNA intermediate. Cell. 1985 Mar;40(3):491–500. doi: 10.1016/0092-8674(85)90197-7. [DOI] [PubMed] [Google Scholar]
  2. Boeke J. D., LaCroute F., Fink G. R. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet. 1984;197(2):345–346. doi: 10.1007/BF00330984. [DOI] [PubMed] [Google Scholar]
  3. Bruschi C. V., Esposito M. S. Enhancement of spontaneous mitotic recombination by the meiotic mutant spo11-1 in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7566–7570. doi: 10.1073/pnas.80.24.7566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chaleff D. T., Fink G. R. Genetic events associated with an insertion mutation in yeast. Cell. 1980 Aug;21(1):227–237. doi: 10.1016/0092-8674(80)90130-0. [DOI] [PubMed] [Google Scholar]
  5. Chattoo B. B., Sherman F., Azubalis D. A., Fjellstedt T. A., Mehnert D., Ogur M. Selection of lys2 Mutants of the Yeast SACCHAROMYCES CEREVISIAE by the Utilization of alpha-AMINOADIPATE. Genetics. 1979 Sep;93(1):51–65. doi: 10.1093/genetics/93.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ciriacy M., Williamson V. M. Analysis of mutations affecting Ty-mediated gene expression in Saccharomyces cerevisiae. Mol Gen Genet. 1981;182(1):159–163. doi: 10.1007/BF00422784. [DOI] [PubMed] [Google Scholar]
  7. Clark-Adams C. D., Winston F. The SPT6 gene is essential for growth and is required for delta-mediated transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Feb;7(2):679–686. doi: 10.1128/mcb.7.2.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Davidow L. S., Byers B. Enhanced gene conversion and postmeiotic segregation in pachytene-arrested Saccharomyces cerevisiae. Genetics. 1984 Feb;106(2):165–183. doi: 10.1093/genetics/106.2.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eibel H., Gafner J., Stotz A., Philippsen P. Characterization of the yeast mobile element Ty1. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):609–617. doi: 10.1101/sqb.1981.045.01.079. [DOI] [PubMed] [Google Scholar]
  10. Farabaugh P. J., Fink G. R. Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication. Nature. 1980 Jul 24;286(5771):352–356. doi: 10.1038/286352a0. [DOI] [PubMed] [Google Scholar]
  11. Fink G. R., Styles C. A. Gene conversion of deletions in the his4 region of yeast. Genetics. 1974 Jun;77(2):231–244. doi: 10.1093/genetics/77.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hamza H., Nicolas A., Rossignol J. L. Large Heterologies Impose Their Gene Conversion Pattern onto Closely Linked Point Mutations. Genetics. 1987 May;116(1):45–53. doi: 10.1093/genetics/116.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holliday R. Gene conversion: a possible mechanism for eliminating selfish DNA. Basic Life Sci. 1982;20:259–264. doi: 10.1007/978-1-4613-3476-7_17. [DOI] [PubMed] [Google Scholar]
  14. Jackson J. A., Fink G. R. Gene conversion between duplicated genetic elements in yeast. Nature. 1981 Jul 23;292(5821):306–311. doi: 10.1038/292306a0. [DOI] [PubMed] [Google Scholar]
  15. Jinks-Robertson S., Petes T. D. Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics. 1986 Nov;114(3):731–752. doi: 10.1093/genetics/114.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Leblon G. Mechanism of gene conversion in Ascobolus immersus. II. The relationships between the genetic alterations in b 1 or b 2 mutants and their conversion spectrum. Mol Gen Genet. 1972;116(4):322–335. doi: 10.1007/BF00270089. [DOI] [PubMed] [Google Scholar]
  17. Losson R., Lacroute F. Interference of nonsense mutations with eukaryotic messenger RNA stability. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5134–5137. doi: 10.1073/pnas.76.10.5134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McKnight G. L., Cardillo T. S., Sherman F. An extensive deletion causing overproduction of yeast iso-2-cytochrome c. Cell. 1981 Aug;25(2):409–419. doi: 10.1016/0092-8674(81)90059-3. [DOI] [PubMed] [Google Scholar]
  19. Meselson M. S., Radding C. M. A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975 Jan;72(1):358–361. doi: 10.1073/pnas.72.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Orr-Weaver T. L., Szostak J. W., Rothstein R. J. Genetic applications of yeast transformation with linear and gapped plasmids. Methods Enzymol. 1983;101:228–245. doi: 10.1016/0076-6879(83)01017-4. [DOI] [PubMed] [Google Scholar]
  21. Paquin C. E., Williamson V. M. Temperature effects on the rate of ty transposition. Science. 1984 Oct 5;226(4670):53–55. doi: 10.1126/science.226.4670.53. [DOI] [PubMed] [Google Scholar]
  22. Pukkila P. J., Stephens M. D., Binninger D. M., Errede B. Frequency and directionality of gene conversion events involving the CYC7-H3 mutation in Saccharomyces cerevisiae. Genetics. 1986 Oct;114(2):347–361. doi: 10.1093/genetics/114.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Roeder G. S., Fink G. R. DNA rearrangements associated with a transposable element in yeast. Cell. 1980 Aug;21(1):239–249. doi: 10.1016/0092-8674(80)90131-2. [DOI] [PubMed] [Google Scholar]
  24. Roeder G. S., Smith M., Lambie E. J. Intrachromosomal movement of genetically marked Saccharomyces cerevisiae transposons by gene conversion. Mol Cell Biol. 1984 Apr;4(4):703–711. doi: 10.1128/mcb.4.4.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rose M., Winston F. Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene. Mol Gen Genet. 1984;193(3):557–560. doi: 10.1007/BF00382100. [DOI] [PubMed] [Google Scholar]
  26. Rothstein R. Deletions of a tyrosine tRNA gene in S. cerevisiae. Cell. 1979 May;17(1):185–190. doi: 10.1016/0092-8674(79)90306-4. [DOI] [PubMed] [Google Scholar]
  27. Scherer S., Davis R. W. Replacement of chromosome segments with altered DNA sequences constructed in vitro. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951–4955. doi: 10.1073/pnas.76.10.4951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Silverman S. J., Fink G. R. Effects of Ty insertions on HIS4 transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Jul;4(7):1246–1251. doi: 10.1128/mcb.4.7.1246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Simchen G., Winston F., Styles C. A., Fink G. R. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2431–2434. doi: 10.1073/pnas.81.8.2431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Szostak J. W., Orr-Weaver T. L., Rothstein R. J., Stahl F. W. The double-strand-break repair model for recombination. Cell. 1983 May;33(1):25–35. doi: 10.1016/0092-8674(83)90331-8. [DOI] [PubMed] [Google Scholar]
  31. Winston F., Chaleff D. T., Valent B., Fink G. R. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics. 1984 Jun;107(2):179–197. doi: 10.1093/genetics/107.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]

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