Abstract
The genetic organization of interval 62B3-4 to 62D3-4 on the Drosophila third chromosome was investigated. The region (designated DRE) includes four known loci: Roughened (R; 3-1.4), defined by a dominant mutation disrupting eye morphology; the nonvital locus Aprt, structural gene for adenine phosphoribosyltranferase; Dras3, a homolog of the vertebrate ras oncogene; and l(3)ecdysoneless (l(3)ecd), a gene that has been implicated in the regulation of larval molting hormone (ecdysteroid) synthesis. Overlapping chromosomal deletions of the region were generated by γ-ray-induced reversion of the R mutation. Recessive lethal mutations were isolated based upon failure to complement the recessive lethality of Df(3L)R(R2), a deletion of the DRE region that removes 16-18 polytene chromosome bands. A total of 117 mutations were isolated following ethyl methanesulfonate and γ-ray mutagenesis. These and two additional mutations define 13 lethal complementation groups. Mutations at two loci were recovered at disproportionately high rates. One of these loci is preferentially sensitive to radiation-induced mutational alterations. Additionally, an unusually low recovery rate for cytologically detectable rearrangement breakpoints within the γ-ray-sensitive locus suggests that an interval of the DRE region closely linked to the R locus may be dominantly sensitive to position effects. Lethal phase analysis of mutant hemizygotes indicates that a high proportion of DRE-region loci (11 of 13) are necessary for larval development. Mutations in five loci cause predominantly first-instar larval lethality, while mutations in four other loci cause predominantly second-instar lethality. Mutations in two loci cause late-larval lethality associated with abnormal imaginal disc development. A temperature-sensitive allele of one newly identified complementation group blocks ecdysteroid-induced pupariation. This developmental block is overcome by dietary 20-hydroxyecdysone, suggesting that a second locus in the region in addition to l(3)ecd may play a role in the regulation of late larval ecdysteroid levels.
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ashburner M. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. I. Autosomal puffing patterns in a laboratory stock of Drosophila melanogaster. Chromosoma. 1967;21(4):398–428. doi: 10.1007/BF00336950. [DOI] [PubMed] [Google Scholar]
- Barrett J. A. The estimation of the number of mutationally silent loci in saturation-mapping experiments. Genet Res. 1980 Feb;35(1):33–44. doi: 10.1017/s0016672300013914. [DOI] [PubMed] [Google Scholar]
- Cooley L., Kelley R., Spradling A. Insertional mutagenesis of the Drosophila genome with single P elements. Science. 1988 Mar 4;239(4844):1121–1128. doi: 10.1126/science.2830671. [DOI] [PubMed] [Google Scholar]
- Duncan I. W., Kaufman T. C. Cytogenic analysis of chromosome 3 in Drosophila melanogaster: mapping of the proximal portion of the right arm. Genetics. 1975 Aug;80(4):733–752. doi: 10.1093/genetics/80.4.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hazelrigg T., Kaufman T. C. Revertants of Dominant Mutations Associated with the Antennapedia Gene Complex of DROSOPHILA MELANOGASTER: Cytology and Genetics. Genetics. 1983 Nov;105(3):581–600. doi: 10.1093/genetics/105.3.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henrich V. C., Tucker R. L., Maroni G., Gilbert L. I. The ecdysoneless (ecd1ts) mutation disrupts ecdysteroid synthesis autonomously in the ring gland of Drosophila melanogaster. Dev Biol. 1987 Mar;120(1):50–55. doi: 10.1016/0012-1606(87)90102-3. [DOI] [PubMed] [Google Scholar]
- Jack J. W. Molecular organization of the cut locus of Drosophila melanogaster. Cell. 1985 Oct;42(3):869–876. doi: 10.1016/0092-8674(85)90283-1. [DOI] [PubMed] [Google Scholar]
- Johnson D. H., Edström J. E., Burnett J. B., Friedman T. B. Cloning of a Drosophila melanogaster adenine phosphoribosyltransferase structural gene and deduced amino acid sequence of the enzyme. Gene. 1987;59(1):77–86. doi: 10.1016/0378-1119(87)90268-x. [DOI] [PubMed] [Google Scholar]
- Judd B. H., Shen M. W., Kaufman T. C. The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics. 1972 May;71(1):139–156. doi: 10.1093/genetics/71.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lefevre G. The distribution of randomly recovered X-ray-induced sex-linked genetic effects in Drosophila melanogaster. Genetics. 1981 Nov-Dec;99(3-4):461–480. doi: 10.1093/genetics/99.3-4.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lefevre G., Watkins W. The question of the total gene number in Drosophila melanogaster. Genetics. 1986 Aug;113(4):869–895. doi: 10.1093/genetics/113.4.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mohler J., Pardue M. L. Mutational Analysis of the Region Surrounding the 93d Heat Shock Locus of DROSOPHILA MELANOGASTER. Genetics. 1984 Feb;106(2):249–265. doi: 10.1093/genetics/106.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neuman-Silberberg F. S., Schejter E., Hoffmann F. M., Shilo B. Z. The Drosophila ras oncogenes: structure and nucleotide sequence. Cell. 1984 Jul;37(3):1027–1033. doi: 10.1016/0092-8674(84)90437-9. [DOI] [PubMed] [Google Scholar]
- Segal D., Shilo B. Z. Tissue localization of Drosophila melanogaster ras transcripts during development. Mol Cell Biol. 1986 Jun;6(6):2241–2248. doi: 10.1128/mcb.6.6.2241. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sliter T. J. Imaginal disc-autonomous expression of a defect in sensory bristle patterning caused by the lethal(3)ecdysoneless1 (1(3)ecd1) mutation of Drosophila melanogaster. Development. 1989 Jun;106(2):347–354. doi: 10.1242/dev.106.2.347. [DOI] [PubMed] [Google Scholar]