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. 1989 Oct;123(2):417–425. doi: 10.1093/genetics/123.2.417

Molecular Analysis of a Transposon-Induced Deletion of the Nivea Locus in Antirrhinum Majus

C Lister 1, C Martin 1
PMCID: PMC1203813  PMID: 2555255

Abstract

The transposable element Tam3 of Antirrhinum majus is capable of causing large-scale chromosomal restructuring. It induced a large deletion at the nivea locus, to produce the allele niv(-):529. The deletion removed the entire nivea coding region while the element remains intact with the potential to induce further rearrangements. Genetic experiments showed that the endpoint of the deletion (called x) is closely linked to nivea. The DNA sequences of niv(-):529, a genomic excision of Tam3 from niv(-):529, and the original genomic position of x have been determined. These data suggest that the deletion could have resulted from an abortive transposition or through breakage and religation.

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Selected References

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  1. Berg R., Engels W. R., Kreber R. A. Site-specific X-chromosome rearrangements from hybrid dysgenesis in Drosophila melanogaster. Science. 1980 Oct;210(4468):427–429. doi: 10.1126/science.6776625. [DOI] [PubMed] [Google Scholar]
  2. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Calos M. P., Miller J. H. Transposable elements. Cell. 1980 Jul;20(3):579–595. doi: 10.1016/0092-8674(80)90305-0. [DOI] [PubMed] [Google Scholar]
  4. Coen E. S., Carpenter R. A semi-dominant allele, niv-525, acts in trans to inhibit expression of its wild-type homologue in Antirrhinum majus. EMBO J. 1988 Apr;7(4):877–883. doi: 10.1002/j.1460-2075.1988.tb02891.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coen E. S., Carpenter R., Martin C. Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus. Cell. 1986 Oct 24;47(2):285–296. doi: 10.1016/0092-8674(86)90451-4. [DOI] [PubMed] [Google Scholar]
  6. Coupland G., Baker B., Schell J., Starlinger P. Characterization of the maize transposable element Ac by internal deletions. EMBO J. 1988 Dec 1;7(12):3653–3659. doi: 10.1002/j.1460-2075.1988.tb03246.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dooner H. K., English J., Ralston E. J. The frequency of transposition of the maize element Activator is not affected by an adjacent deletion. Mol Gen Genet. 1988 Mar;211(3):485–491. doi: 10.1007/BF00425705. [DOI] [PubMed] [Google Scholar]
  8. Ising G., Block K. Derivation-dependent distribution of insertion sites for a Drosophila transposon. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):527–544. doi: 10.1101/sqb.1981.045.01.069. [DOI] [PubMed] [Google Scholar]
  9. Martin C., Carpenter R., Sommer H., Saedler H., Coen E. S. Molecular analysis of instability in flower pigmentation of Antirrhinum majus, following isolation of the pallida locus by transposon tagging. EMBO J. 1985 Jul;4(7):1625–1630. doi: 10.1002/j.1460-2075.1985.tb03829.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martin C., Mackay S., Carpenter R. Large-scale chromosomal restructuring is induced by the transposable element tam3 at the nivea locus of antirrhinum majus. Genetics. 1988 May;119(1):171–184. doi: 10.1093/genetics/119.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin C., Prescott A., Lister C., MacKay S. Activity of the transposon Tam3 in Antirrhinum and tobacco: possible role of DNA methylation. EMBO J. 1989 Apr;8(4):997–1004. doi: 10.1002/j.1460-2075.1989.tb03466.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pohlman R. F., Fedoroff N. V., Messing J. The nucleotide sequence of the maize controlling element Activator. Cell. 1984 Jun;37(2):635–643. doi: 10.1016/0092-8674(84)90395-7. [DOI] [PubMed] [Google Scholar]
  13. Saedler H., Nevers P. Transposition in plants: a molecular model. EMBO J. 1985 Mar;4(3):585–590. doi: 10.1002/j.1460-2075.1985.tb03670.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schiefelbein J. W., Furtek D. B., Dooner H. K., Nelson O. E., Jr Two mutations in a maize bronze-1 allele caused by transposable elements of the Ac-Ds family alter the quantity and quality of the gene product. Genetics. 1988 Nov;120(3):767–777. doi: 10.1093/genetics/120.3.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Will B. M., Bayev A. A., Finnegan D. J. Nucleotide sequence of terminal repeats of 412 transposable elements of Drosophila melanogaster. A similarity to proviral long terminal repeats and its implications for the mechanism of transposition. J Mol Biol. 1981 Dec 25;153(4):897–915. doi: 10.1016/0022-2836(81)90458-7. [DOI] [PubMed] [Google Scholar]

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