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. 1990 Jan;124(1):145–156. doi: 10.1093/genetics/124.1.145

Aristapedioid: A Gain of Function, Homeotic Mutation in Drosophila Melanogaster

B P Brunk 1, P N Adler 1
PMCID: PMC1203901  PMID: 1968409

Abstract

The isolation of gain of function mutations has allowed the identification of a number of genes which are important in the normal development of the organism. We report here the isolation and characterization of Aristapedioid, a gain of function mutation which causes a partial transformation of arista towards tarsus and the loss or decrease in size of the dorso-central and scutellar bristles. Aristapedioid is the result of a P element mediated inversion which juxtaposes unrelated DNA adjacent to Suppressor 2 of zeste, causing a gain of function mutation in that gene.

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Selected References

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  1. Adler P. N., Charlton J., Brunk B. Genetic interactions of the suppressor 2 of zeste region genes. Dev Genet. 1989;10(3):249–260. doi: 10.1002/dvg.1020100314. [DOI] [PubMed] [Google Scholar]
  2. Adler P. N., MacQueen M. Cell proliferation and DNA replication in the imaginal wing disc of Drosophila melanogaster. Dev Biol. 1984 May;103(1):28–37. doi: 10.1016/0012-1606(84)90004-6. [DOI] [PubMed] [Google Scholar]
  3. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  4. Biggin M. D., Bickel S., Benson M., Pirrotta V., Tjian R. Zeste encodes a sequence-specific transcription factor that activates the Ultrabithorax promoter in vitro. Cell. 1988 Jun 3;53(5):713–722. doi: 10.1016/0092-8674(88)90089-x. [DOI] [PubMed] [Google Scholar]
  5. Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
  6. Cohen S. M., Brönner G., Küttner F., Jürgens G., Jäckle H. Distal-less encodes a homoeodomain protein required for limb development in Drosophila. Nature. 1989 Mar 30;338(6214):432–434. doi: 10.1038/338432a0. [DOI] [PubMed] [Google Scholar]
  7. Denell R. E. Homoeosis in Drosophila. I. Complementation studies with revertants of Nasobemia. Genetics. 1973 Oct;75(2):279–297. doi: 10.1093/genetics/75.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duncan I. M. Polycomblike: a gene that appears to be required for the normal expression of the bithorax and antennapedia gene complexes of Drosophila melanogaster. Genetics. 1982 Sep;102(1):49–70. doi: 10.1093/genetics/102.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Engels W. R., Preston C. R. Formation of chromosome rearrangements by P factors in Drosophila. Genetics. 1984 Aug;107(4):657–678. doi: 10.1093/genetics/107.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Engels W. R. The P family of transposable elements in Drosophila. Annu Rev Genet. 1983;17:315–344. doi: 10.1146/annurev.ge.17.120183.001531. [DOI] [PubMed] [Google Scholar]
  11. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  12. Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
  13. Frischer L. E., Hagen F. S., Garber R. L. An inversion that disrupts the Antennapedia gene causes abnormal structure and localization of RNAs. Cell. 1986 Dec 26;47(6):1017–1023. doi: 10.1016/0092-8674(86)90816-0. [DOI] [PubMed] [Google Scholar]
  14. Garber R. L., Kuroiwa A., Gehring W. J. Genomic and cDNA clones of the homeotic locus Antennapedia in Drosophila. EMBO J. 1983;2(11):2027–2036. doi: 10.1002/j.1460-2075.1983.tb01696.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gelbart W. M., Wu C. T. Interactions of zeste mutations with loci exhibiting transvection effects in Drosophila melanogaster. Genetics. 1982 Oct;102(2):179–189. doi: 10.1093/genetics/102.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ghysen A., Dambly-Chaudière C. From DNA to form: the achaete-scute complex. Genes Dev. 1988 May;2(5):495–501. doi: 10.1101/gad.2.5.495. [DOI] [PubMed] [Google Scholar]
  17. Kalisch W. E., Rasmuson B. Changes of zeste phenotype induced by autosomal mutations in Drosophila melanogaster. Hereditas. 1974;78(1):97–104. doi: 10.1111/j.1601-5223.1974.tb01432.x. [DOI] [PubMed] [Google Scholar]
  18. Kuziora M. A., McGinnis W. Autoregulation of a Drosophila homeotic selector gene. Cell. 1988 Nov 4;55(3):477–485. doi: 10.1016/0092-8674(88)90034-7. [DOI] [PubMed] [Google Scholar]
  19. Lasko P. F., Pardue M. L. Studies of the genetic organization of the vestigial microregion of Drosophila melanogaster. Genetics. 1988 Oct;120(2):495–502. doi: 10.1093/genetics/120.2.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Levis R., Rubin G. M. The unstable wDZL mutation of Drosophila is caused by a 13 kilobase insertion that is imprecisely excised in phenotypic revertants. Cell. 1982 Sep;30(2):543–550. doi: 10.1016/0092-8674(82)90251-3. [DOI] [PubMed] [Google Scholar]
  21. Lewis E. B. A gene complex controlling segmentation in Drosophila. Nature. 1978 Dec 7;276(5688):565–570. doi: 10.1038/276565a0. [DOI] [PubMed] [Google Scholar]
  22. Lewis R. A., Kaufman T. C., Denell R. E., Tallerico P. Genetic Analysis of the Antennapedia Gene Complex (Ant-C) and Adjacent Chromosomal Regions of DROSOPHILA MELANOGASTER. I. Polytene Chromosome Segments 84b-D. Genetics. 1980 Jun;95(2):367–381. doi: 10.1093/genetics/95.2.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lewis R. A., Wakimoto B. T., Denell R. E., Kaufman T. C. Genetic Analysis of the Antennapedia Gene Complex (Ant-C) and Adjacent Chromosomal Regions of DROSOPHILA MELANOGASTER. II. Polytene Chromosome Segments 84A-84B1,2. Genetics. 1980 Jun;95(2):383–397. doi: 10.1093/genetics/95.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nüsslein-Volhard C., Wieschaus E. Mutations affecting segment number and polarity in Drosophila. Nature. 1980 Oct 30;287(5785):795–801. doi: 10.1038/287795a0. [DOI] [PubMed] [Google Scholar]
  25. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  26. Rubin G. M., Kidwell M. G., Bingham P. M. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982 Jul;29(3):987–994. doi: 10.1016/0092-8674(82)90462-7. [DOI] [PubMed] [Google Scholar]
  27. Schneuwly S., Klemenz R., Gehring W. J. Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia. 1987 Feb 26-Mar 4Nature. 325(6107):816–818. doi: 10.1038/325816a0. [DOI] [PubMed] [Google Scholar]
  28. Schneuwly S., Kuroiwa A., Gehring W. J. Molecular analysis of the dominant homeotic Antennapedia phenotype. EMBO J. 1987 Jan;6(1):201–206. doi: 10.1002/j.1460-2075.1987.tb04739.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schupbach T., Wieschaus E. Germline autonomy of maternal-effect mutations altering the embryonic body pattern of Drosophila. Dev Biol. 1986 Feb;113(2):443–448. doi: 10.1016/0012-1606(86)90179-x. [DOI] [PubMed] [Google Scholar]
  30. Searles L. L., Jokerst R. S., Bingham P. M., Voelker R. A., Greenleaf A. L. Molecular cloning of sequences from a Drosophila RNA polymerase II locus by P element transposon tagging. Cell. 1982 Dec;31(3 Pt 2):585–592. doi: 10.1016/0092-8674(82)90314-2. [DOI] [PubMed] [Google Scholar]
  31. Seeger M. A., Haffley L., Kaufman T. C. Characterization of amalgam: a member of the immunoglobulin superfamily from Drosophila. Cell. 1988 Nov 18;55(4):589–600. doi: 10.1016/0092-8674(88)90217-6. [DOI] [PubMed] [Google Scholar]
  32. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  33. Spradling A. C., Rubin G. M. Transposition of cloned P elements into Drosophila germ line chromosomes. Science. 1982 Oct 22;218(4570):341–347. doi: 10.1126/science.6289435. [DOI] [PubMed] [Google Scholar]
  34. Struhl G. A gene product required for correct initiation of segmental determination in Drosophila. Nature. 1981 Sep 3;293(5827):36–41. doi: 10.1038/293036a0. [DOI] [PubMed] [Google Scholar]
  35. Struhl G. A homoeotic mutation transforming leg to antenna in Drosophila. Nature. 1981 Aug 13;292(5824):635–638. doi: 10.1038/292635a0. [DOI] [PubMed] [Google Scholar]
  36. Swaroop A., Paco-Larson M. L., Garen A. Molecular genetics of a transposon-induced dominant mutation in the Drosophila locus Glued. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1751–1755. doi: 10.1073/pnas.82.6.1751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Vinson C. R., Conover S., Adler P. N. A Drosophila tissue polarity locus encodes a protein containing seven potential transmembrane domains. Nature. 1989 Mar 16;338(6212):263–264. doi: 10.1038/338263a0. [DOI] [PubMed] [Google Scholar]
  38. Wu C. T., Jones R. S., Lasko P. F., Gelbart W. M. Homeosis and the interaction of zeste and white in Drosophila. Mol Gen Genet. 1989 Sep;218(3):559–564. doi: 10.1007/BF00332424. [DOI] [PubMed] [Google Scholar]

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