Abstract
Restriction site variation in mitochondrial DNA (mtDNA) of the American oyster (Crassostrea virginica) was surveyed in continuously distributed populations sampled from the Gulf of St. Lawrence, Canada, to Brownsville, Texas. mtDNA clonal diversity was high, with 82 different haplotypes revealed among 212 oysters with 13 endonucleases. The mtDNA clones grouped into two distinct genetic arrays (estimated to differ by about 2.6% in nucleotide sequence) that characterized oysters collected north vs. south of a region on the Atlantic mid-coast of Florida. The population genetic ``break'' in mtDNA contrasts with previous reports of near uniformity of nuclear (allozyme) allele frequencies throughout the range of the species, but agrees closely with the magnitude and pattern of mtDNA differentiation reported in other estuarine species in the southeastern United States. This concordance of mtDNA phylogenetic pattern across independently evolving species provides strong evidence for vicariant biogeographic processes in initiating intraspecific population structure. The post-Miocene ecological history of the region suggests that reduced precipitation levels in an enlarged Floridian peninsula may have created discontinuities in suitable estuarine habitat for oysters during glacial periods, and that today such population separations are maintained by the combined influence of ecological gradients and oceanic currents on larval dispersal. The results are consistent with the hypothesis that historical vicariant events, in conjunction with contemporary environmental influences on gene flow, can result in genetic discontinuities in continuously distributed species with high dispersal capability.
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Selected References
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- Avise J. C., Ball R. M., Arnold J. Current versus historical population sizes in vertebrate species with high gene flow: a comparison based on mitochondrial DNA lineages and inbreeding theory for neutral mutations. Mol Biol Evol. 1988 Jul;5(4):331–344. doi: 10.1093/oxfordjournals.molbev.a040504. [DOI] [PubMed] [Google Scholar]
- Avise J. C., Nelson W. S. Molecular genetic relationships of the extinct dusky seaside sparrow. Science. 1989 Feb 3;243(4891):646–648. doi: 10.1126/science.243.4891.646. [DOI] [PubMed] [Google Scholar]
- Bermingham E., Lamb T., Avise J. C. Size polymorphism and heteroplasmy in the mitochondrial DNA of lower vertebrates. J Hered. 1986 Jul-Aug;77(4):249–252. doi: 10.1093/oxfordjournals.jhered.a110230. [DOI] [PubMed] [Google Scholar]
- Birky C. W., Jr, Maruyama T., Fuerst P. An approach to population and evolutionary genetic theory for genes in mitochondria and chloroplasts, and some results. Genetics. 1983 Mar;103(3):513–527. doi: 10.1093/genetics/103.3.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown W. M., George M., Jr, Wilson A. C. Rapid evolution of animal mitochondrial DNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1967–1971. doi: 10.1073/pnas.76.4.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coyne J. A. Lack of genic similarity between two sibling species of drosophila as revealed by varied techniques. Genetics. 1976 Nov;84(3):593–607. doi: 10.1093/genetics/84.3.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drouin J. Cloning of human mitochondrial DNA in Escherichia coli. J Mol Biol. 1980 Jun 15;140(1):15–34. doi: 10.1016/0022-2836(80)90354-x. [DOI] [PubMed] [Google Scholar]
- Gyllensten U., Wharton D., Wilson A. C. Maternal inheritance of mitochondrial DNA during backcrossing of two species of mice. J Hered. 1985 Sep-Oct;76(5):321–324. doi: 10.1093/oxfordjournals.jhered.a110103. [DOI] [PubMed] [Google Scholar]
- KORRINGA P. Recent advances in oyster biology. Q Rev Biol. 1952 Sep;27(3):266–contd. doi: 10.1086/399023. [DOI] [PubMed] [Google Scholar]
- Nei M., Li W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269–5273. doi: 10.1073/pnas.76.10.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nei M., Tajima F. DNA polymorphism detectable by restriction endonucleases. Genetics. 1981 Jan;97(1):145–163. doi: 10.1093/genetics/97.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vawter L., Brown W. M. Nuclear and mitochondrial DNA comparisons reveal extreme rate variation in the molecular clock. Science. 1986 Oct 10;234(4773):194–196. doi: 10.1126/science.3018931. [DOI] [PubMed] [Google Scholar]