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. 1990 Mar;124(3):615–625. doi: 10.1093/genetics/124.3.615

Pairing for Recombination in Lgv of Caenorhabditis Elegans: A Model Based on Recombination in Deficiency Heterozygotes

R E Rosenbluth 1, R C Johnsen 1, D L Baillie 1
PMCID: PMC1203955  PMID: 2311918

Abstract

The effect of deficiencies on recombination was studied in Caenorhabditis elegans. Heterozygous deficiencies in the left half of linkage group V [LGV(left)] were shown to inhibit recombination to their right. Fourteen deficiencies, all to the left of unc-46, were analyzed for their effect on recombination along LGV. The deficiencies fell into two groups: 10 ``major inhibitors'' which reduce recombination to less than 11% of the expected rate between themselves and unc-46; and four ``minor inhibitors'' which reduce recombination, but to a much lesser extent. All four minor inhibitors delete the left-most known gene on the chromosome, while six of the ten major inhibitors do not (i.e., these are ``internal'' deficiencies). Where recombination could be measured on both sides of a deficiency, recombination was inhibited to the right but not to the left. In order to explain these results we have erected a model for the manner in which pairing for recombination takes place. In doing so, we identify a new region of LGV, near the left terminus, that is important for the pairing process.

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Selected References

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  1. Beadle G. W. A Possible Influence of the Spindle Fibre on Crossing-Over in Drosophila. Proc Natl Acad Sci U S A. 1932 Feb;18(2):160–165. doi: 10.1073/pnas.18.2.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
  3. Blackman R. K., Grimaila R., Koehler M. M., Gelbart W. M. Mobilization of hobo elements residing within the decapentaplegic gene complex: suggestion of a new hybrid dysgenesis system in Drosophila melanogaster. Cell. 1987 May 22;49(4):497–505. doi: 10.1016/0092-8674(87)90452-1. [DOI] [PubMed] [Google Scholar]
  4. Bridges C B, Skoog E N, Li J C. Genetical and Cytological Studies of a Deficiency (Notopleural) in the Second Chromosome of Drosophila Melanogaster. Genetics. 1936 Nov;21(6):788–795. doi: 10.1093/genetics/21.6.788. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bucheton A., Paro R., Sang H. M., Pelisson A., Finnegan D. J. The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor. Cell. 1984 Aug;38(1):153–163. doi: 10.1016/0092-8674(84)90536-1. [DOI] [PubMed] [Google Scholar]
  6. Chovnick A., Ballantyne G. H., Holm D. G. Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster. Genetics. 1971 Oct;69(2):179–209. doi: 10.1093/genetics/69.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clark D. V., Rogalski T. M., Donati L. M., Baillie D. L. The unc-22(IV) region of Caenorhabditis elegans: genetic analysis of lethal mutations. Genetics. 1988 Jun;119(2):345–353. doi: 10.1093/genetics/119.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clark S. H., McCarron M., Love C., Chovnick A. On the identification of the rosy locus DNA in Drosophila melanogaster: intragenic recombination mapping of mutations associated with insertions and deletions. Genetics. 1986 Apr;112(4):755–767. doi: 10.1093/genetics/112.4.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferguson E. L., Horvitz H. R. Identification and characterization of 22 genes that affect the vulval cell lineages of the nematode Caenorhabditis elegans. Genetics. 1985 May;110(1):17–72. doi: 10.1093/genetics/110.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Finnegan D. J., Fawcett D. H. Transposable elements in Drosophila melanogaster. Oxf Surv Eukaryot Genes. 1986;3:1–62. [PubMed] [Google Scholar]
  11. Finnegan D. J. Transposable elements in eukaryotes. Int Rev Cytol. 1985;93:281–326. doi: 10.1016/s0074-7696(08)61376-5. [DOI] [PubMed] [Google Scholar]
  12. Goldstein P., Slaton D. E. The synaptonemal complexes of caenorhabditis elegans: comparison of wild-type and mutant strains and pachytene karyotype analysis of wild-type. Chromosoma. 1982;84(4):585–597. doi: 10.1007/BF00292857. [DOI] [PubMed] [Google Scholar]
  13. Herman R. K. Crossover suppressors and balanced recessive lethals in Caenorhabditis elegans. Genetics. 1978 Jan;88(1):49–65. doi: 10.1093/genetics/88.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Herman R. K., Kari C. K., Hartman P. S. Dominant X-chromosome nondisjunction mutants of Caenorhabditis elegans. Genetics. 1982 Nov;102(3):379–400. doi: 10.1093/genetics/102.3.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Herman R. K., Kari C. K. Recombination between small X chromosome duplications and the X chromosome in Caenorhabditis elegans. Genetics. 1989 Apr;121(4):723–737. doi: 10.1093/genetics/121.4.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Horvitz H. R., Brenner S., Hodgkin J., Herman R. K. A uniform genetic nomenclature for the nematode Caenorhabditis elegans. Mol Gen Genet. 1979 Sep;175(2):129–133. doi: 10.1007/BF00425528. [DOI] [PubMed] [Google Scholar]
  17. Johnsen R. C., Baillie D. L. Formaldehyde mutagenesis of the eT1 balanced region in Caenorhabditis elegans: dose-response curve and the analysis of mutational events. Mutat Res. 1988 Sep;201(1):137–147. doi: 10.1016/0027-5107(88)90120-0. [DOI] [PubMed] [Google Scholar]
  18. Jones G. H. The control of chiasma distribution. Symp Soc Exp Biol. 1984;38:293–320. [PubMed] [Google Scholar]
  19. Kidwell M. G., Kidwell J. F., Sved J. A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977 Aug;86(4):813–833. doi: 10.1093/genetics/86.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lefevre G., Jr, Moore L. B. Recombination in regions adjacent to deletions in the X chromosome of Drosophila melanogaster. Genetics. 1968 Apr;58(4):557–571. doi: 10.1093/genetics/58.4.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mackay T. F. Transposable elements and fitness in Drosophila melanogaster. Genome. 1989;31(1):284–295. doi: 10.1139/g89-046. [DOI] [PubMed] [Google Scholar]
  22. McKim K. S., Howell A. M., Rose A. M. The effects of translocations on recombination frequency in Caenorhabditis elegans. Genetics. 1988 Dec;120(4):987–1001. doi: 10.1093/genetics/120.4.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mori I., Moerman D. G., Waterston R. H. Analysis of a mutator activity necessary for germline transposition and excision of Tc1 transposable elements in Caenorhabditis elegans. Genetics. 1988 Oct;120(2):397–407. doi: 10.1093/genetics/120.2.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moses M. J., Dresser M. E., Poorman P. A. Composition and role of the synaptonemal complex. Symp Soc Exp Biol. 1984;38:245–270. [PubMed] [Google Scholar]
  25. Roberts P. A. Screening for x-ray-induced crossover suppressors in Drosophila melanogaster: prevalence and effectiveness of translocations. Genetics. 1970 Jul;65(3):429–448. doi: 10.1093/genetics/65.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rogalski T. M., Bullerjahn A. M., Riddle D. L. Lethal and amanitin-resistance mutations in the Caenorhabditis elegans ama-1 and ama-2 genes. Genetics. 1988 Oct;120(2):409–422. doi: 10.1093/genetics/120.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rose A. M., Baillie D. L., Curran J. Meiotic pairing behavior of two free duplications of linkage group I in Caenorhabditis elegans. Mol Gen Genet. 1984;195(1-2):52–56. doi: 10.1007/BF00332723. [DOI] [PubMed] [Google Scholar]
  28. Rose A. M., Baillie D. L. The Effect of Temperature and Parental Age on Recombination and Nondisjunction in CAENORHABDITIS ELEGANS. Genetics. 1979 Jun;92(2):409–418. doi: 10.1093/genetics/92.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rosenbluth R. E., Baillie D. L. The genetic analysis of a reciprocal translocation, eT1(III; V), in Caenorhabditis elegans. Genetics. 1981 Nov-Dec;99(3-4):415–428. doi: 10.1093/genetics/99.3-4.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stadler L. J., Roman H. The Effect of X-Rays upon Mutation of the Gene A in Maize. Genetics. 1948 May;33(3):273–303. doi: 10.1093/genetics/33.3.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yamamoto M., Miklos G. L. Genetic studies on heterochromatin in Drosophila melanogaster and their implications for the functions of satellite DNA. Chromosoma. 1978 Mar 22;66(1):71–98. doi: 10.1007/BF00285817. [DOI] [PubMed] [Google Scholar]
  32. Yannopoulos G., Stamatis N., Monastirioti M., Hatzopoulos P., Louis C. hobo is responsible for the induction of hybrid dysgenesis by strains of Drosophila melanogaster bearing the male recombination factor 23.5MRF. Cell. 1987 May 22;49(4):487–495. doi: 10.1016/0092-8674(87)90451-x. [DOI] [PubMed] [Google Scholar]
  33. von Wettstein D., Rasmussen S. W., Holm P. B. The synaptonemal complex in genetic segregation. Annu Rev Genet. 1984;18:331–413. doi: 10.1146/annurev.ge.18.120184.001555. [DOI] [PubMed] [Google Scholar]

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