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. 1990 Mar;124(3):663–676. doi: 10.1093/genetics/124.3.663

Repression of P Element-Mediated Hybrid Dysgenesis in Drosophila Melanogaster

M J Simmons 1, J D Raymond 1, K E Rasmusson 1, L M Miller 1, C F McLarnon 1, J R Zunt 1
PMCID: PMC1203959  PMID: 2155854

Abstract

Inbred lines derived from a strain called Sexi were analyzed for their abilities to repress P element-mediated gonadal dysgenesis. One line had high repression ability, four had intermediate ability and two had very low ability. The four intermediate lines also exhibited considerable within-line variation for this trait; furthermore, in at least two cases, this variation could not be attributed to recurring P element movement. Repression of gonadal dysgenesis in the hybrid offspring of all seven lines was due primarily to a maternal effect; there was no evidence for repression arising de novo in the hybrids themselves. In one of the lines, repression ability was inherited maternally, indicating the involvement of cytoplasmic factors. In three other lines, repression ability appeared to be determined by partially dominant or additive chromosomal factors; however, there was also evidence for a maternal effect that reduced the expression of these factors in at least two of the lines. In another line, repression ability seemed to be due to recessive chromosomal factors. All seven lines possessed numerous copies of a particular P element, called KP, which has been hypothesized to produce a polypeptide repressor of gonadal dysgenesis. This hypothesis, however, does not explain why the inbred Sexi lines varied so much in their repression abilities. It is suggested that some of this variation may be due to differences in the chromosomal position of the KP elements, or that other nonautonomous P elements are involved in the repression of hybrid dysgenesis in these lines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anxolabéhère D., Nouaud D., Périquet G., Tchen P. P-element distribution in Eurasian populations of Drosophila melanogaster: A genetic and molecular analysis. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5418–5422. doi: 10.1073/pnas.82.16.5418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Daniels S. B., Clark S. H., Kidwell M. G., Chovnick A. Genetic transformation of Drosophila melanogaster with an autonomous P element: phenotypic and molecular analyses of long-established transformed lines. Genetics. 1987 Apr;115(4):711–723. doi: 10.1093/genetics/115.4.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Engels W. R., Benz W. K., Preston C. R., Graham P. L., Phillis R. W., Robertson H. M. Somatic effects of P element activity in Drosophila melanogaster: pupal lethality. Genetics. 1987 Dec;117(4):745–757. doi: 10.1093/genetics/117.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engels W. R. Germline hypermutability in Drosophila and its relation to hybrid dysgenesis and cytotype. Genetics. 1981 Jul;98(3):565–587. doi: 10.1093/genetics/98.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Engels W. R. Hybrid dysgenesis in Drosophila and the Stochastic loss hypothesis. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):561–565. doi: 10.1101/sqb.1981.045.01.072. [DOI] [PubMed] [Google Scholar]
  6. Jackson M. S., Black D. M., Dover G. A. Amplification of KP elements associated with the repression of hybrid dysgenesis in Drosophila melanogaster. Genetics. 1988 Dec;120(4):1003–1013. doi: 10.1093/genetics/120.4.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karess R. E., Rubin G. M. Analysis of P transposable element functions in Drosophila. Cell. 1984 Aug;38(1):135–146. doi: 10.1016/0092-8674(84)90534-8. [DOI] [PubMed] [Google Scholar]
  8. Kidwell M. G. Hybrid dysgenesis in Drosophila melanogaster: nature and inheritance of P element regulation. Genetics. 1985 Oct;111(2):337–350. doi: 10.1093/genetics/111.2.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kidwell M. G., Kidwell J. F., Sved J. A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977 Aug;86(4):813–833. doi: 10.1093/genetics/86.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kidwell M. G., Novy J. B., Feeley S. M. Rapid unidirectional change of hybrid dysgenesis potential in Drosophila. J Hered. 1981 Jan-Feb;72(1):32–38. doi: 10.1093/oxfordjournals.jhered.a109422. [DOI] [PubMed] [Google Scholar]
  11. Kidwell M. G., Novy J. B. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: Sterility Resulting from Gonadal Dysgenesis in the P-M System. Genetics. 1979 Aug;92(4):1127–1140. doi: 10.1093/genetics/92.4.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kocur G. J., Drier E. A., Simmons M. J. Sterility and hypermutability in the P-M system of hybrid dysgenesis in Drosophila melanogaster. Genetics. 1986 Dec;114(4):1147–1163. doi: 10.1093/genetics/114.4.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laski F. A., Rio D. C., Rubin G. M. Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing. Cell. 1986 Jan 17;44(1):7–19. doi: 10.1016/0092-8674(86)90480-0. [DOI] [PubMed] [Google Scholar]
  14. Nitasaka E., Mukai T., Yamazaki T. Repressor of P elements in Drosophila melanogaster: Cytotype determination by a defective P element carrying only open reading frames 0 through 2. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7605–7608. doi: 10.1073/pnas.84.21.7605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Hare K., Rubin G. M. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. doi: 10.1016/0092-8674(83)90133-2. [DOI] [PubMed] [Google Scholar]
  16. Preston C. R., Engels W. R. Spread of P transposable elements in inbred lines of Drosophila melanogaster. Prog Nucleic Acid Res Mol Biol. 1989;36:71–85. doi: 10.1016/s0079-6603(08)60162-2. [DOI] [PubMed] [Google Scholar]
  17. Rasmusson K. E., Simmons M. J., Raymond J. D., McLarnon C. F. Quantitative effects of P elements on hybrid dysgenesis in Drosophila melanogaster. Genetics. 1990 Mar;124(3):647–662. doi: 10.1093/genetics/124.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rio D. C., Laski F. A., Rubin G. M. Identification and immunochemical analysis of biologically active Drosophila P element transposase. Cell. 1986 Jan 17;44(1):21–32. doi: 10.1016/0092-8674(86)90481-2. [DOI] [PubMed] [Google Scholar]
  19. Robertson H. M., Engels W. R. Modified P elements that mimic the P cytotype in Drosophila melanogaster. Genetics. 1989 Dec;123(4):815–824. doi: 10.1093/genetics/123.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Roiha H., Rubin G. M., O'Hare K. P element insertions and rearrangements at the singed locus of Drosophila melanogaster. Genetics. 1988 May;119(1):75–83. doi: 10.1093/genetics/119.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ronsseray S., Anxolabéhère D., Périquet G. Hybrid dysgenesis in Drosophila melanogaster: influence of temperature on cytotype determination in the P-M system. Mol Gen Genet. 1984;196(1):17–23. doi: 10.1007/BF00334086. [DOI] [PubMed] [Google Scholar]
  22. Simmons M. J., Johnson N. A., Fahey T. M., Nellett S. M., Raymond J. D. High mutability in male hybrids of Drosophila melanogaster. Genetics. 1980 Oct;96(2):479–480. doi: 10.1093/genetics/96.2.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Simmons M. J., Raymond J. D., Boedigheimer M. J., Zunt J. R. The influence of nonautonomous P elements on hybrid dysgenesis in Drosophila melanogaster. Genetics. 1987 Dec;117(4):671–685. doi: 10.1093/genetics/117.4.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Simmons M. J., Raymond J. D., Culbert T. P., Laverty T. R. Analysis of dysgenesis-induced lethal mutations on the X chromosome of a Q strain of Drosophila melanogaster. Genetics. 1984 May;107(1):49–63. doi: 10.1093/genetics/107.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Simmons M. J., Raymond J. D., Laverty T. R., Doll R. F., Raymond N. C., Kocur G. J., Drier E. A. Chromosomal effects on mutability in the P-M system of hybrid dysgenesis in Drosophila melanogaster. Genetics. 1985 Dec;111(4):869–884. doi: 10.1093/genetics/111.4.869. [DOI] [PMC free article] [PubMed] [Google Scholar]

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