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. 1990 Apr;124(4):873–880. doi: 10.1093/genetics/124.4.873

Developmental Expression of the Glucose Dehydrogenase Gene in Drosophila Melanogaster

D L Cox-Foster 1, C P Schonbaum 1, M T Murtha 1, D R Cavener 1
PMCID: PMC1203978  PMID: 2108903

Abstract

The Gld gene of Drosophila melanogaster is transiently expressed during every stage of development. The temporal pattern of Gld expression is highly correlated with that of ecdysteroids. Exogeneous treatment of third instar larvae with 20-hydroxyecdysone induces the accumulation of Gld mRNA in the hypoderm and anterior spiracular gland cells. During metamorphosis Gld is expressed in a variety of tissues derived from the ectoderm. In the developing reproductive tract, Gld mRNA accumulates in the female spermathecae and oviduct and in the male ejaculatory duct and ejaculatory bulb. These four organs are derived from closely related cell lineages in the genital imaginal disc. Since the expression of Gld is not required for the development of these reproductive structures, this spatial pattern of expression is most likely a fortuitous consequence of a shared regulatory factor in this cell lineage. At the adult stage a high level of the Gld mRNA is only observed in the male ejaculatory duct.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bainbridge S. P., Bownes M. Staging the metamorphosis of Drosophila melanogaster. J Embryol Exp Morphol. 1981 Dec;66:57–80. [PubMed] [Google Scholar]
  2. Baker B. S., Ridge K. A. Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster. Genetics. 1980 Feb;94(2):383–423. doi: 10.1093/genetics/94.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Belote J. M., Handler A. M., Wolfner M. F., Livak K. J., Baker B. S. Sex-specific regulation of yolk protein gene expression in Drosophila. Cell. 1985 Feb;40(2):339–348. doi: 10.1016/0092-8674(85)90148-5. [DOI] [PubMed] [Google Scholar]
  4. Cavener D. R. Coevolution of the glucose dehydrogenase gene and the ejaculatory duct in the genus Drosophila. Mol Biol Evol. 1985 Mar;2(2):141–149. doi: 10.1093/oxfordjournals.molbev.a040344. [DOI] [PubMed] [Google Scholar]
  5. Cavener D. R., MacIntyre R. J. Biphasic expression and function of glucose dehydrogenase in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6286–6288. doi: 10.1073/pnas.80.20.6286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cavener D., Corbett G., Cox D., Whetten R. Isolation of the eclosion gene cluster and the developmental expression of the Gld gene in Drosophila melanogaster. EMBO J. 1986 Nov;5(11):2939–2948. doi: 10.1002/j.1460-2075.1986.tb04590.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DiBenedetto A. J., Lakich D. M., Kruger W. D., Belote J. M., Baker B. S., Wolfner M. F. Sequences expressed sex-specifically in Drosophila melanogaster adults. Dev Biol. 1987 Jan;119(1):242–251. doi: 10.1016/0012-1606(87)90225-9. [DOI] [PubMed] [Google Scholar]
  8. Epper F., Nöthiger R. Genetic and developmental evidence for a repressed genital primordium in Drosophila melanogaster. Dev Biol. 1982 Nov;94(1):163–175. doi: 10.1016/0012-1606(82)90079-3. [DOI] [PubMed] [Google Scholar]
  9. Hafen E., Levine M., Garber R. L., Gehring W. J. An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex. EMBO J. 1983;2(4):617–623. doi: 10.1002/j.1460-2075.1983.tb01472.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirsh J., Morgan B. A., Scholnick S. B. Delimiting regulatory sequences of the Drosophila melanogaster Ddc gene. Mol Cell Biol. 1986 Dec;6(12):4548–4557. doi: 10.1128/mcb.6.12.4548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kornberg T., Sidén I., O'Farrell P., Simon M. The engrailed locus of Drosophila: in situ localization of transcripts reveals compartment-specific expression. Cell. 1985 Jan;40(1):45–53. doi: 10.1016/0092-8674(85)90307-1. [DOI] [PubMed] [Google Scholar]
  12. Kraminsky G. P., Clark W. C., Estelle M. A., Gietz R. D., Sage B. A., O'Connor J. D., Hodgetts R. B. Induction of translatable mRNA for dopa decarboxylase in Drosophila: an early response to ecdysterone. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4175–4179. doi: 10.1073/pnas.77.7.4175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lynn D. A., Angerer L. M., Bruskin A. M., Klein W. H., Angerer R. C. Localization of a family of MRNAS in a single cell type and its precursors in sea urchin embryos. Proc Natl Acad Sci U S A. 1983 May;80(9):2656–2660. doi: 10.1073/pnas.80.9.2656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marsh J. L., Wright T. R. Developmental relationship between dopa decarboxylase, dopamine acetyltransferase, and ecdysone in Drosophila. Dev Biol. 1980 Dec;80(2):379–387. doi: 10.1016/0012-1606(80)90412-1. [DOI] [PubMed] [Google Scholar]
  15. Mitchison T. J., Sedat J. Localization of antigenic determinants in whole Drosophila embryos. Dev Biol. 1983 Sep;99(1):261–264. doi: 10.1016/0012-1606(83)90275-0. [DOI] [PubMed] [Google Scholar]
  16. Murtha M. T., Cavener D. R. Ecdysteroid regulation of glucose dehydrogenase and alcohol dehydrogenase gene expression in Drosophila melanogaster. Dev Biol. 1989 Sep;135(1):66–73. doi: 10.1016/0012-1606(89)90158-9. [DOI] [PubMed] [Google Scholar]
  17. White K., Kankel D. R. Patterns of cell division and cell movement in the formation of the imaginal nervous system in Drosophila melanogaster. Dev Biol. 1978 Aug;65(2):296–321. doi: 10.1016/0012-1606(78)90029-5. [DOI] [PubMed] [Google Scholar]

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