Abstract
Saccharomyces cerevisiae cells containing a deletion of TRK1, the gene encoding the high affinity potassium transporter, retain only low affinity uptake of this ion and consequently lose the ability to grow in media containing low levels (0.2 mM) of potassium. Using a trk1Δ strain, we selected spontaneous Trk(+) pseudorevertants that regained the ability to grow on low concentrations of potassium. The revertants define three unlinked extragenic suppressors of trk1Δ. Dominant RPD2 mutations and recessive rpd1 and rpd3 mutations confer increased potassium uptake in trk1Δ cells. Genetic evidence suggests that RPD2 mutations are alleles of TRK2, the putative low affinity transporter gene, whereas rpd1 and rpd3 mutations increase TRK2 activity: (1) RPD2 mutations are closely linked to trk2, and (2) trk2 mutations are epistatic to both rpd1 and rpd3. rpd1 maps near pho80 on chromosome XV and rpd3 maps on the left arm of chromosome XIV, closely linked to kre1.
Full Text
The Full Text of this article is available as a PDF (1.9 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Epstein W., Wieczorek L., Siebers A., Altendorf K. Potassium transport in Escherichia coli: genetic and biochemical characterization of the K+-transporting ATPase. Biochem Soc Trans. 1984 Apr;12(2):235–236. doi: 10.1042/bst0120235. [DOI] [PubMed] [Google Scholar]
- Gaber R. F., Copple D. M., Kennedy B. K., Vidal M., Bard M. The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol Cell Biol. 1989 Aug;9(8):3447–3456. doi: 10.1128/mcb.9.8.3447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gaber R. F., Styles C. A., Fink G. R. TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae. Mol Cell Biol. 1988 Jul;8(7):2848–2859. doi: 10.1128/mcb.8.7.2848. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ko C. H., Buckley A. M., Gaber R. F. TRK2 is required for low affinity K+ transport in Saccharomyces cerevisiae. Genetics. 1990 Jun;125(2):305–312. doi: 10.1093/genetics/125.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ma C., Mortimer R. K. Empirical equation that can be used to determine genetic map distances from tetrad data. Mol Cell Biol. 1983 Oct;3(10):1886–1887. doi: 10.1128/mcb.3.10.1886. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCusker J. H., Perlin D. S., Haber J. E. Pleiotropic plasma membrane ATPase mutations of Saccharomyces cerevisiae. Mol Cell Biol. 1987 Nov;7(11):4082–4088. doi: 10.1128/mcb.7.11.4082. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mortimer R. K., Schild D., Contopoulou C. R., Kans J. A. Genetic map of Saccharomyces cerevisiae, edition 10. Yeast. 1989 Sep-Oct;5(5):321–403. doi: 10.1002/yea.320050503. [DOI] [PubMed] [Google Scholar]
- Perkins D. D. Biochemical Mutants in the Smut Fungus Ustilago Maydis. Genetics. 1949 Sep;34(5):607–626. doi: 10.1093/genetics/34.5.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salkoff L., Wyman R. Genetic modification of potassium channels in Drosophila Shaker mutants. Nature. 1981 Sep 17;293(5829):228–230. doi: 10.1038/293228a0. [DOI] [PubMed] [Google Scholar]
- Serrano R., Kielland-Brandt M. C., Fink G. R. Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases. Nature. 1986 Feb 20;319(6055):689–693. doi: 10.1038/319689a0. [DOI] [PubMed] [Google Scholar]
- Shih C. K., Wagner R., Feinstein S., Kanik-Ennulat C., Neff N. A dominant trifluoperazine resistance gene from Saccharomyces cerevisiae has homology with F0F1 ATP synthase and confers calcium-sensitive growth. Mol Cell Biol. 1988 Aug;8(8):3094–3103. doi: 10.1128/mcb.8.8.3094. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ulaszewski S., Grenson M., Goffeau A. Modified plasma-membrane ATPase in mutants of Saccharomyces cerevisiae. Eur J Biochem. 1983 Feb 1;130(2):235–239. doi: 10.1111/j.1432-1033.1983.tb07141.x. [DOI] [PubMed] [Google Scholar]