Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1990 Jun;125(2):333–340. doi: 10.1093/genetics/125.2.333

Chromosomal Assignment of Mutations by Specific Chromosome Loss in the Yeast Saccharomyces Cerevisiae

L P Wakem 1, F Sherman 1
PMCID: PMC1204023  PMID: 2199315

Abstract

Yeast 2-μm plasmids were integrated near the centromere of a different chromosome in each of 16 cir(0) mapping strains of Saccharomyces cerevisiae. The specific chromosomes containing the integrated 2-μm plasmid DNA were lost at a high frequency after crossing the cir(0) strains to cir(+) strains. A recessive mutation in a cir(+) strain can then be easily assigned to its chromosome using this set of mapping strains, since the phenotype of the recessive mutation will be manifested only in diploids having the integrated 2-μm plasmid and the unmapped mutation on homologous chromosomes.

Full Text

The Full Text of this article is available as a PDF (1.8 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abovich N., Rosbash M. Two genes for ribosomal protein 51 of Saccharomyces cerevisiae complement and contribute to the ribosomes. Mol Cell Biol. 1984 Sep;4(9):1871–1879. doi: 10.1128/mcb.4.9.1871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beggs J. D. Transformation of yeast by a replicating hybrid plasmid. Nature. 1978 Sep 14;275(5676):104–109. doi: 10.1038/275104a0. [DOI] [PubMed] [Google Scholar]
  3. Boeke J. D., LaCroute F., Fink G. R. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet. 1984;197(2):345–346. doi: 10.1007/BF00330984. [DOI] [PubMed] [Google Scholar]
  4. Botstein D., Falco S. C., Stewart S. E., Brennan M., Scherer S., Stinchcomb D. T., Struhl K., Davis R. W. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene. 1979 Dec;8(1):17–24. doi: 10.1016/0378-1119(79)90004-0. [DOI] [PubMed] [Google Scholar]
  5. Carlson M., Botstein D. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase. Cell. 1982 Jan;28(1):145–154. doi: 10.1016/0092-8674(82)90384-1. [DOI] [PubMed] [Google Scholar]
  6. Costanzo M. C., Seaver E. C., Fox T. D. The PET54 gene of Saccharomyces cerevisiae: characterization of a nuclear gene encoding a mitochondrial translational activator and subcellular localization of its product. Genetics. 1989 Jun;122(2):297–305. doi: 10.1093/genetics/122.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Falco S. C., Li Y., Broach J. R., Botstein D. Genetic properties of chromosomally integrated 2 mu plasmid DNA in yeast. Cell. 1982 Jun;29(2):573–584. doi: 10.1016/0092-8674(82)90173-8. [DOI] [PubMed] [Google Scholar]
  8. Falco S. C., Rose M., Botstein D. Homologous Recombination between Episomal Plasmids and Chromosomes in Yeast. Genetics. 1983 Dec;105(4):843–856. doi: 10.1093/genetics/105.4.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gaber R. F., Mathison L., Edelman I., Culbertson M. R. Frameshift Suppression in SACCHAROMYCES CEREVISIAE VI. Complete Genetic Map of Twenty-Five Suppressor Genes. Genetics. 1983 Mar;103(3):389–407. doi: 10.1093/genetics/103.3.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hieter P., Pridmore D., Hegemann J. H., Thomas M., Davis R. W., Philippsen P. Functional selection and analysis of yeast centromeric DNA. Cell. 1985 Oct;42(3):913–921. doi: 10.1016/0092-8674(85)90287-9. [DOI] [PubMed] [Google Scholar]
  11. Hinnebusch A. G., Fink G. R. Positive regulation in the general amino acid control of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5374–5378. doi: 10.1073/pnas.80.17.5374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klapholz S., Esposito R. E. A new mapping method employing a meiotic rec-mutant of yeast. Genetics. 1982 Mar;100(3):387–412. doi: 10.1093/genetics/100.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Liljelund P., Losson R., Kammerer B., Lacroute F. Yeast regulatory gene PPR1. II. Chromosomal localization, meiotic map, suppressibility, dominance/recessivity and dosage effect. J Mol Biol. 1984 Dec 5;180(2):251–265. doi: 10.1016/s0022-2836(84)80003-0. [DOI] [PubMed] [Google Scholar]
  14. Liras P., McCusker J., Mascioli S., Haber J. E. Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis. Genetics. 1978 Apr;88(4 Pt 1):651–671. [PMC free article] [PubMed] [Google Scholar]
  15. Mortimer R. K., Contopoulou R., Schild D. Mitotic chromosome loss in a radiation-sensitive strain of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5778–5782. doi: 10.1073/pnas.78.9.5778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ono B., Shirahige Y., Nanjoh A., Andou N., Ohue H., Ishino-Arao Y. Cysteine biosynthesis in Saccharomyces cerevisiae: mutation that confers cystathionine beta-synthase deficiency. J Bacteriol. 1988 Dec;170(12):5883–5889. doi: 10.1128/jb.170.12.5883-5889.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rudolph H. K., Antebi A., Fink G. R., Buckley C. M., Dorman T. E., LeVitre J., Davidow L. S., Mao J. I., Moir D. T. The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family. Cell. 1989 Jul 14;58(1):133–145. doi: 10.1016/0092-8674(89)90410-8. [DOI] [PubMed] [Google Scholar]
  18. Schild D., Mortimer R. K. A mapping method for Saccharomyces cerevisiae using rad52-induced chromosome loss. Genetics. 1985 Aug;110(4):569–589. doi: 10.1093/genetics/110.4.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Toyn J., Hibbs A. R., Sanz P., Crowe J., Meyer D. I. In vivo and in vitro analysis of ptl1, a yeast ts mutant with a membrane-associated defect in protein translocation. EMBO J. 1988 Dec 20;7(13):4347–4353. doi: 10.1002/j.1460-2075.1988.tb03333.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wickner R. B. Mapping chromosomal genes of Saccharomyces cerevisiae using an improved genetic mapping method. Genetics. 1979 Jul;92(3):803–821. doi: 10.1093/genetics/92.3.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wood J. S. Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae. Mol Cell Biol. 1982 Sep;2(9):1080–1087. doi: 10.1128/mcb.2.9.1080. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES