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. 1990 Jun;125(2):407–419. doi: 10.1093/genetics/125.2.407

Molecular and Phenotypic Variation of the Zw Locus Region in Drosophila Melanogaster

N T Miyashita 1
PMCID: PMC1204029  PMID: 1974224

Abstract

Restriction map polymorphism in a 13-kb region of the Zw locus in Drosophila melanogaster was investigated for 64 X chromosome lines with seven 6-cutter and ten 4-cutter restriction enzymes. A total of 203 restriction sites were scored, of which 20 were found to be polymorphic. The estimated nucleotide variation for this region for overall data (π = 0.003 and 0.001, and θ = 0.003 and 0.002, for 4-cutter and 6-cutter studies, respectively) was smaller than that reported for most regions studied in D. melanogaster. It was found that the Slow allozyme has a larger nucleotide variation and haplotype diversity than the Fast allozyme. Results suggest the relatively recent divergence of the Fast allozyme from the Slow allozyme. Glucose 6-phosphate dehydrogenase (G6PD) activity was measured as a phenotype of the Zw locus. A significant difference in G6PD activity between allozymes was detected. The between-line effect was highly significant within the Slow allozyme, but was not significant within the Fast allozyme. Although a direct causative link could not be established, these results suggest an association between the amounts of quantitative and molecular genetic variation at the Zw locus region.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguadé M., Miyashita N., Langley C. H. Restriction-map variation at the zeste-tko region in natural populations of Drosophila melanogaster. Mol Biol Evol. 1989 Mar;6(2):123–130. doi: 10.1093/oxfordjournals.molbev.a040538. [DOI] [PubMed] [Google Scholar]
  2. Brown A. J. Variation at the 87A heat shock locus in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5350–5354. doi: 10.1073/pnas.80.17.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cavener D. R., Clegg M. T. Evidence for biochemical and physiological differences between enzyme genotypes in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4444–4447. doi: 10.1073/pnas.78.7.4444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cross S. R., Birley A. J. Restriction endonuclease map variation in the Adh region in populations of Drosophila melanogaster. Biochem Genet. 1986 Jun;24(5-6):415–433. doi: 10.1007/BF00499097. [DOI] [PubMed] [Google Scholar]
  5. Eanes W. F., Ajioka J. W., Hey J., Wesley C. Restriction-map variation associated with the G6PD polymorphism in natural populations of Drosophila melanogaster. Mol Biol Evol. 1989 Jul;6(4):384–397. doi: 10.1093/oxfordjournals.molbev.a040555. [DOI] [PubMed] [Google Scholar]
  6. Eanes W. F., Bingham B., Hey J., Houle D. Targeted selection experiments and enzyme polymorphism: negative evidence for octanoate selection at the G6PD locus in Drosophila melanogaster. Genetics. 1985 Feb;109(2):379–391. doi: 10.1093/genetics/109.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Engels W. R. Estimating genetic divergence and genetic variability with restriction endonucleases. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6329–6333. doi: 10.1073/pnas.78.10.6329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fouts D., Ganguly R., Gutierrez A. G., Lucchesi J. C., Manning J. E. Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene. Gene. 1988 Mar 31;63(2):261–275. doi: 10.1016/0378-1119(88)90530-6. [DOI] [PubMed] [Google Scholar]
  9. Kaplan N. L., Hudson R. R., Langley C. H. The "hitchhiking effect" revisited. Genetics. 1989 Dec;123(4):887–899. doi: 10.1093/genetics/123.4.887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kreitman M., Aguadé M. Genetic uniformity in two populations of Drosophila melanogaster as revealed by filter hybridization of four-nucleotide-recognizing restriction enzyme digests. Proc Natl Acad Sci U S A. 1986 May;83(10):3562–3566. doi: 10.1073/pnas.83.10.3562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Langley C. H., Montgomery E., Quattlebaum W. F. Restriction map variation in the Adh region of Drosophila. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5631–5635. doi: 10.1073/pnas.79.18.5631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Langley C. H., Shrimpton A. E., Yamazaki T., Miyashita N., Matsuo Y., Aquadro C. F. Naturally occurring variation in the restriction map of the amy region of Drosophila melanogaster. Genetics. 1988 Jul;119(3):619–629. doi: 10.1093/genetics/119.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laurie-Ahlberg C. C., Maroni G., Bewley G. C., Lucchesi J. C., Weir B. S. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073–1077. doi: 10.1073/pnas.77.2.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laurie-Ahlberg C. C., Stam L. F. Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster. Genetics. 1987 Jan;115(1):129–140. doi: 10.1093/genetics/115.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laurie-Ahlberg C. C., Weir B. S. Allozymic Variation and Linkage Disequilibrium in Some Laboratory Populations of DROSOPHILA MELANOGASTER. Genetics. 1979 Aug;92(4):1295–1314. doi: 10.1093/genetics/92.4.1295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lucchesi J. C., Hughes M. B., Geer B. W. Genetic control of pentose phosphate pathway enzymes in Drosophila. Curr Top Cell Regul. 1979;15:143–154. doi: 10.1016/b978-0-12-152815-7.50008-9. [DOI] [PubMed] [Google Scholar]
  18. Maruyama T. Effective number of alleles in a subdivided population. Theor Popul Biol. 1970 Nov;1(3):273–306. doi: 10.1016/0040-5809(70)90047-x. [DOI] [PubMed] [Google Scholar]
  19. Miyashita N., Langley C. H. Molecular and phenotypic variation of the white locus region in Drosophila melanogaster. Genetics. 1988 Sep;120(1):199–212. doi: 10.1093/genetics/120.1.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Miyashita N., Laurie-Ahlberg C. C. Genetical analysis of chromosomal interaction effects on the activities of the glucose 6-phosphate and 6-phosphogluconate dehydrogenases in Drosophila melanogaster. Genetics. 1984 Apr;106(4):655–668. doi: 10.1093/genetics/106.4.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nei M., Li W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269–5273. doi: 10.1073/pnas.76.10.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nei M., Tajima F. DNA polymorphism detectable by restriction endonucleases. Genetics. 1981 Jan;97(1):145–163. doi: 10.1093/genetics/97.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith J. M., Haigh J. The hitch-hiking effect of a favourable gene. Genet Res. 1974 Feb;23(1):23–35. [PubMed] [Google Scholar]
  24. Steele M. W., Young W. J., Childs B. Glucose 6-phosphate dehydrogenase in Drosophila malanogaster: starch gel electrophoretic variation due to molecular instability. Biochem Genet. 1968 Sep;2(2):159–175. doi: 10.1007/BF01458714. [DOI] [PubMed] [Google Scholar]
  25. Stephan W., Langley C. H. Molecular genetic variation in the centromeric region of the X chromosome in three Drosophila ananassae populations. I. Contrasts between the vermilion and forked loci. Genetics. 1989 Jan;121(1):89–99. doi: 10.1093/genetics/121.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stewart B. R., Merriam J. R. Segmental aneuploidy and enzyme activity as a method for cytogenetic localization in drosophila melanogaster. Genetics. 1974 Feb;76(2):301–309. doi: 10.1093/genetics/76.2.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. YOUNG W. J., PORTER J. E., CHILDS B. GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN DROSOPHILA: X-LINKED ELECTROPHORETIC VARIANTS. Science. 1964 Jan 10;143(3602):140–141. doi: 10.1126/science.143.3602.140. [DOI] [PubMed] [Google Scholar]

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