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. 1990 Oct;126(2):387–400. doi: 10.1093/genetics/126.2.387

Characterization of the Fb-Nof Transposable Element of Drosophila Melanogaster

N Harden 1, M Ashburner 1
PMCID: PMC1204193  PMID: 2174013

Abstract

FB-NOF is a composite transposable element of Drosophila melanogaster. It is composed of foldback sequences, of variable length, which flank a 4-kb NOF sequence with 308-bp inverted repeat termini. The NOF sequence could potentially code for a 120-kD polypeptide. The FB-NOF element is responsible for unstable mutations of the white gene (w(c) and w(DZL)) and is associated with the large TEs of G. Ising. Although most strains of D. melanogaster have 20-30 sites of FB insertion, FB-NOF elements are usually rare, many strains lack this composite element or have only one copy of it. A few strains, including w(DZL) and Basc have many (8-21) copies of FB-NOF, and these show a tendency to insert at ``hot-spots.'' These strains also have an increased number of FB elements. The DNA sequence of the NOF region associated with TE146(Z) has been determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bingham P. M. The Regulation of White Locus Expression: A Dominant Mutant Allele at the White Locus of DROSOPHILA MELANOGASTER. Genetics. 1980 Jun;95(2):341–353. doi: 10.1093/genetics/95.2.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brierley H. L., Potter S. S. Distinct characteristics of loop sequences of two Drosophila foldback transposable elements. Nucleic Acids Res. 1985 Jan 25;13(2):485–500. doi: 10.1093/nar/13.2.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Campuzano S., Balcells L., Villares R., Carramolino L., García-Alonso L., Modolell J. Excess function hairy-wing mutations caused by gypsy and copia insertions within structural genes of the achaete-scute locus of Drosophila. Cell. 1986 Jan 31;44(2):303–312. doi: 10.1016/0092-8674(86)90764-6. [DOI] [PubMed] [Google Scholar]
  4. Chia W., McGill S., Karp R., Gubb D., Ashburner M. Spontaneous excision of a large composite transposable element of Drosophila melanogaster. Nature. 1985 Jul 4;316(6023):81–83. doi: 10.1038/316081a0. [DOI] [PubMed] [Google Scholar]
  5. Collins M., Rubin G. M. High-frequency precise excision of the Drosophila foldback transposable element. Nature. 1983 May 19;303(5914):259–260. doi: 10.1038/303259a0. [DOI] [PubMed] [Google Scholar]
  6. Collins M., Rubin G. M. Structure of chromosomal rearrangements induced by the FB transposable element in Drosophila. Nature. 1984 Mar 22;308(5957):323–327. doi: 10.1038/308323a0. [DOI] [PubMed] [Google Scholar]
  7. Collins M., Rubin G. M. Structure of the Drosophila mutable allele, white-crimson, and its white-ivory and wild-type derivatives. Cell. 1982 Aug;30(1):71–79. doi: 10.1016/0092-8674(82)90013-7. [DOI] [PubMed] [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Finnegan D. J., Fawcett D. H. Transposable elements in Drosophila melanogaster. Oxf Surv Eukaryot Genes. 1986;3:1–62. [PubMed] [Google Scholar]
  10. Goldberg M. L., Paro R., Gehring W. J. Molecular cloning of the white locus region of Drosophila melanogaster using a large transposable element. EMBO J. 1982;1(1):93–98. doi: 10.1002/j.1460-2075.1982.tb01130.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green M. M. Controlling element mediated transpositions of the white gene in Drosophila melanogaster. Genetics. 1969 Feb;61(2):429–441. doi: 10.1093/genetics/61.2.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gubb D., Roote J., McGill S., Shelton M., Ashburner M. Interactions between WHITE Genes Carried by a Large Transposing Element and the ZESTE Allele in DROSOPHILA MELANOGASTER. Genetics. 1986 Mar;112(3):551–575. doi: 10.1093/genetics/112.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Harris L. J., Baillie D. L., Rose A. M. Sequence identity between an inverted repeat family of transposable elements in Drosophila and Caenorhabditis. Nucleic Acids Res. 1988 Jul 11;16(13):5991–5998. doi: 10.1093/nar/16.13.5991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Henikoff S., Plasterk R. H. Related transposons in C.elegans and D.melanogaster. Nucleic Acids Res. 1988 Jul 11;16(13):6234–6234. doi: 10.1093/nar/16.13.6234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ising G., Block K. A transposon as a cytogenetic marker in Drosophila melanogaster. Mol Gen Genet. 1984;196(1):6–16. doi: 10.1007/BF00334085. [DOI] [PubMed] [Google Scholar]
  16. Ising G., Block K. Derivation-dependent distribution of insertion sites for a Drosophila transposon. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):527–544. doi: 10.1101/sqb.1981.045.01.069. [DOI] [PubMed] [Google Scholar]
  17. Jakubczak J. L., Xiong Y., Eickbush T. H. Type I (R1) and type II (R2) ribosomal DNA insertions of Drosophila melanogaster are retrotransposable elements closely related to those of Bombyx mori. J Mol Biol. 1990 Mar 5;212(1):37–52. doi: 10.1016/0022-2836(90)90303-4. [DOI] [PubMed] [Google Scholar]
  18. Levis R., Bingham P. M., Rubin G. M. Physical map of the white locus of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Jan;79(2):564–568. doi: 10.1073/pnas.79.2.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Levis R., Collins M., Rubin G. M. FB elements are the common basis for the instability of the wDZL and wC Drosophila mutations. Cell. 1982 Sep;30(2):551–565. doi: 10.1016/0092-8674(82)90252-5. [DOI] [PubMed] [Google Scholar]
  20. Levis R., Rubin G. M. The unstable wDZL mutation of Drosophila is caused by a 13 kilobase insertion that is imprecisely excised in phenotypic revertants. Cell. 1982 Sep;30(2):543–550. doi: 10.1016/0092-8674(82)90251-3. [DOI] [PubMed] [Google Scholar]
  21. Liebermann D. A., Hoffman-Liebermann B. Proto-oncogene expression and dissection of the myeloid growth to differentiation developmental cascade. Oncogene. 1989 May;4(5):583–592. [PubMed] [Google Scholar]
  22. O'Hare K., Murphy C., Levis R., Rubin G. M. DNA sequence of the white locus of Drosophila melanogaster. J Mol Biol. 1984 Dec 15;180(3):437–455. doi: 10.1016/0022-2836(84)90021-4. [DOI] [PubMed] [Google Scholar]
  23. Paro R., Goldberg M. L., Gehring W. J. Molecular analysis of large transposable elements carrying the white locus of Drosophila melanogaster. EMBO J. 1983;2(6):853–860. doi: 10.1002/j.1460-2075.1983.tb01513.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Potter S. S. DNA sequence of a foldback transposable element in Drosophila. Nature. 1982 May 20;297(5863):201–204. doi: 10.1038/297201a0. [DOI] [PubMed] [Google Scholar]
  25. Potter S., Truett M., Phillips M., Maher A. Eucaryotic transposable genetic elements with inverted terminal repeats. Cell. 1980 Jul;20(3):639–647. doi: 10.1016/0092-8674(80)90310-4. [DOI] [PubMed] [Google Scholar]
  26. Schmid C. W., Manning J. E., Davidson N. Inverted repeat sequences in the Drosophila genome. Cell. 1975 Jun;5(2):159–172. doi: 10.1016/0092-8674(75)90024-0. [DOI] [PubMed] [Google Scholar]
  27. Shields D. C., Sharp P. M. Evidence that mutation patterns vary among Drosophila transposable elements. J Mol Biol. 1989 Jun 20;207(4):843–846. doi: 10.1016/0022-2836(89)90252-0. [DOI] [PubMed] [Google Scholar]
  28. Silber J., Bazin C., Lemeunier F., Aulard S., Volovitch M. Distribution and conservation of the foldback transposable element in Drosophila. J Mol Evol. 1989 Mar;28(3):220–224. doi: 10.1007/BF02102479. [DOI] [PubMed] [Google Scholar]
  29. Staden R. Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521–538. doi: 10.1093/nar/12.1part2.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Templeton N. S., Potter S. S. Complete foldback transposable elements encode a novel protein found in Drosophila melanogaster. EMBO J. 1989 Jun;8(6):1887–1894. doi: 10.1002/j.1460-2075.1989.tb03585.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Truett M. A., Jones R. S., Potter S. S. Unusual structure of the FB family of transposable elements in Drosophila. Cell. 1981 Jun;24(3):753–763. doi: 10.1016/0092-8674(81)90101-x. [DOI] [PubMed] [Google Scholar]
  32. Zachar Z., Bingham P. M. Regulation of white locus expression: the structure of mutant alleles at the white locus of Drosophila melanogaster. Cell. 1982 Sep;30(2):529–541. doi: 10.1016/0092-8674(82)90250-1. [DOI] [PubMed] [Google Scholar]

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