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. 1991 Apr;127(4):739–746. doi: 10.1093/genetics/127.4.739

Gene Conversion in Drosophila and the Effects of the Meiotic Mutants Mei-9 and Mei-218

D Curtis 1, W Bender 1
PMCID: PMC1204401  PMID: 2029970

Abstract

Simple meiotic gene conversion tracts produced in wild-type females were compared with those from two meiotic mutants, mei-9 and mei-218. The positions and lengths of conversion tracts were determined by denaturing gradient gels and DNA sequencing. Conversion tracts in wild type averaged 885 base pairs in length, were continuous, and displayed no obvious hot spots of initiation. Some unusual conversion events were found in the mei-218 and mei-9 samples, although most events were indistinguishable from wild-type tracts in their length and continuity.

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Selected References

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  1. Baker B. S., Carpenter A. T., Esposito M. S., Esposito R. E., Sandler L. The genetic control of meiosis. Annu Rev Genet. 1976;10:53–134. doi: 10.1146/annurev.ge.10.120176.000413. [DOI] [PubMed] [Google Scholar]
  2. Bender W., Spierer P., Hogness D. S. Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. J Mol Biol. 1983 Jul 25;168(1):17–33. doi: 10.1016/s0022-2836(83)80320-9. [DOI] [PubMed] [Google Scholar]
  3. Borts R. H., Haber J. E. Length and distribution of meiotic gene conversion tracts and crossovers in Saccharomyces cerevisiae. Genetics. 1989 Sep;123(1):69–80. doi: 10.1093/genetics/123.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boyd J. B., Golino M. D., Setlow R. B. The mei-9 alpha mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair. Genetics. 1976 Nov;84(3):527–544. doi: 10.1093/genetics/84.3.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boyd J. B., Mason J. M., Yamamoto A. H., Brodberg R. K., Banga S. S., Sakaguchi K. A genetic and molecular analysis of DNA repair in Drosophila. J Cell Sci Suppl. 1987;6:39–60. doi: 10.1242/jcs.1984.supplement_6.3. [DOI] [PubMed] [Google Scholar]
  6. Carpenter A. T. Electron microscopy of meiosis in Drosophila melanogaster females: II. The recombination nodule--a recombination-associated structure at pachytene? Proc Natl Acad Sci U S A. 1975 Aug;72(8):3186–3189. doi: 10.1073/pnas.72.8.3186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carpenter A. T. Mismatch repair, gene conversion, and crossing-over in two recombination-defective mutants of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5961–5965. doi: 10.1073/pnas.79.19.5961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Carpenter A. T. Recombination nodules and synaptonemal complex in recombination-defective females of Drosophila melanogaster. Chromosoma. 1979;75(3):259–292. doi: 10.1007/BF00293472. [DOI] [PubMed] [Google Scholar]
  9. Carpenter A. T., Sandler L. On recombination-defective meiotic mutants in Drosophila melanogaster. Genetics. 1974 Mar;76(3):453–475. doi: 10.1093/genetics/76.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chovnick A., Ballantyne G. H., Holm D. G. Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster. Genetics. 1971 Oct;69(2):179–209. doi: 10.1093/genetics/69.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Clark S. H., Hilliker A. J., Chovnick A. Recombination can initiate and terminate at a large number of sites within the rosy locus of Drosophila melanogaster. Genetics. 1988 Feb;118(2):261–266. doi: 10.1093/genetics/118.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Coté B., Bender W., Curtis D., Chovnick A. Molecular mapping of the rosy locus in Drosophila melanogaster. Genetics. 1986 Apr;112(4):769–783. doi: 10.1093/genetics/112.4.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Curtis D., Clark S. H., Chovnick A., Bender W. Molecular analysis of recombination events in Drosophila. Genetics. 1989 Jul;122(3):653–661. doi: 10.1093/genetics/122.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gatti M. Genetic control of chromosome breakage and rejoining in Drosophila melanogaster: spontaneous chromosome aberrations in X-linked mutants defective in DNA metabolism. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1377–1381. doi: 10.1073/pnas.76.3.1377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gray M., Charpentier A., Walsh K., Wu P., Bender W. Mapping point mutations in the Drosophila rosy locus using denaturing gradient gel blots. Genetics. 1991 Jan;127(1):139–149. doi: 10.1093/genetics/127.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hilliker A. J., Chovnick A. Further observations on intragenic recombination in Drosophila melanogaster. Genet Res. 1981 Dec;38(3):281–296. doi: 10.1017/s0016672300020619. [DOI] [PubMed] [Google Scholar]
  17. Judd S. R., Petes T. D. Physical lengths of meiotic and mitotic gene conversion tracts in Saccharomyces cerevisiae. Genetics. 1988 Mar;118(3):401–410. doi: 10.1093/genetics/118.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kalogeropoulos A., Rossignol J. L. Hybrid DNA tracts may start at different sites during meiotic recombination in gene b2 of Ascobolus. EMBO J. 1988 Jan;7(1):253–259. doi: 10.1002/j.1460-2075.1988.tb02807.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Keith T. P., Riley M. A., Kreitman M., Lewontin R. C., Curtis D., Chambers G. Sequence of the structural gene for xanthine dehydrogenase (rosy locus) in Drosophila melanogaster. Genetics. 1987 May;116(1):67–73. doi: 10.1093/genetics/116.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lee C. S., Curtis D., McCarron M., Love C., Gray M., Bender W., Chovnick A. Mutations affecting expression of the rosy locus in Drosophila melanogaster. Genetics. 1987 May;116(1):55–66. doi: 10.1093/genetics/116.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McCarron M., O'Donnell J., Chovnick A., Bhullar B. S., Hewitt J., Candido E. P. Organization of the rosy locus in Drosophila melanogaster: further evidence in support of a cis-acting control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1979 Feb;91(2):275–293. doi: 10.1093/genetics/91.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Meselson M. S., Radding C. M. A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975 Jan;72(1):358–361. doi: 10.1073/pnas.72.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nicolas A., Treco D., Schultes N. P., Szostak J. W. An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiae. Nature. 1989 Mar 2;338(6210):35–39. doi: 10.1038/338035a0. [DOI] [PubMed] [Google Scholar]
  24. Riley M. A. Nucleotide sequence of the Xdh region in Drosophila pseudoobscura and an analysis of the evolution of synonymous codons. Mol Biol Evol. 1989 Jan;6(1):33–52. doi: 10.1093/oxfordjournals.molbev.a040529. [DOI] [PubMed] [Google Scholar]
  25. Symington L. S., Petes T. D. Expansions and contractions of the genetic map relative to the physical map of yeast chromosome III. Mol Cell Biol. 1988 Feb;8(2):595–604. doi: 10.1128/mcb.8.2.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Szostak J. W., Orr-Weaver T. L., Rothstein R. J., Stahl F. W. The double-strand-break repair model for recombination. Cell. 1983 May;33(1):25–35. doi: 10.1016/0092-8674(83)90331-8. [DOI] [PubMed] [Google Scholar]
  27. von Wettstein D., Rasmussen S. W., Holm P. B. The synaptonemal complex in genetic segregation. Annu Rev Genet. 1984;18:331–413. doi: 10.1146/annurev.ge.18.120184.001555. [DOI] [PubMed] [Google Scholar]

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