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. 1991 Aug;128(4):763–775. doi: 10.1093/genetics/128.4.763

Genetic Analysis of Chromosome Region 63 of Drosophila Melanogaster

A D Wohlwill 1, J J Bonner 1
PMCID: PMC1204550  PMID: 1916243

Abstract

The salivary chromosome region including cytological division 63 of Drosophila melanogaster was genetically analyzed in order to (1) characterize this previously unstudied region and (2) attempt to isolate mutations in the hsp82 gene. Seven deletions which span this region were isolated, including four which remove the hsp82 gene. A Minute mutation was mapped to this region and this Minute was used to isolate duplications in the 63 region. These duplications map the Minute to 63B8-C1. F(2) screens were initiated using deletions which remove the hsp82 gene. Over 15,000 chromosomes were screened, yielding 40 lethal mutations which comprise 14 complementation groups. Several of these mutations map outside the 63 region and appear to give second site interaction with the Minute locus. Four loci, including the Minute gene, are candidates for hsp82 mutations by cytogenetic mapping. These loci were tested for complementation with a P element carrying the hsp82 gene. However, none of the mutations was rescued.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashburner M., Chihara C., Meltzer P., Richards G. Temporal control of puffing activity in polytene chromosomes. Cold Spring Harb Symp Quant Biol. 1974;38:655–662. doi: 10.1101/sqb.1974.038.01.070. [DOI] [PubMed] [Google Scholar]
  2. Blackman R. K., Meselson M. Interspecific nucleotide sequence comparisons used to identify regulatory and structural features of the Drosophila hsp82 gene. J Mol Biol. 1986 Apr 20;188(4):499–515. doi: 10.1016/s0022-2836(86)80001-8. [DOI] [PubMed] [Google Scholar]
  3. Bonner J. J., Kerby R. L. RNA polymerase II transcribes all of the heat shock induced genes of Drosophila melanogaster. Chromosoma. 1982;85(1):93–108. doi: 10.1007/BF00344596. [DOI] [PubMed] [Google Scholar]
  4. Borkovich K. A., Farrelly F. W., Finkelstein D. B., Taulien J., Lindquist S. hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures. Mol Cell Biol. 1989 Sep;9(9):3919–3930. doi: 10.1128/mcb.9.9.3919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Broderick D. J., Roberts P. A. Localization of minutes to specific polytene chromosome bands by means of overlapping duplications. Genetics. 1982 Sep;102(1):71–74. doi: 10.1093/genetics/102.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooley L., Kelley R., Spradling A. Insertional mutagenesis of the Drosophila genome with single P elements. Science. 1988 Mar 4;239(4844):1121–1128. doi: 10.1126/science.2830671. [DOI] [PubMed] [Google Scholar]
  7. Dubreuil R. R., Byers T. J., Stewart C. T., Kiehart D. P. A beta-spectrin isoform from Drosophila (beta H) is similar in size to vertebrate dystrophin. J Cell Biol. 1990 Nov;111(5 Pt 1):1849–1858. doi: 10.1083/jcb.111.5.1849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoffman E. P., Corces V. G. Correct temperature induction and developmental regulation of a cloned heat shock gene transformed into the Drosophila germ line. Mol Cell Biol. 1984 Dec;4(12):2883–2889. doi: 10.1128/mcb.4.12.2883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lefevre G., Watkins W. The question of the total gene number in Drosophila melanogaster. Genetics. 1986 Aug;113(4):869–895. doi: 10.1093/genetics/113.4.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leicht B. G., Bonner J. J. Genetic analysis of chromosomal region 67A-D of Drosophila melanogaster. Genetics. 1988 Jul;119(3):579–593. doi: 10.1093/genetics/119.3.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lindsley D. L., Sandler L., Baker B. S., Carpenter A. T., Denell R. E., Hall J. C., Jacobs P. A., Miklos G. L., Davis B. K., Gethmann R. C. Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics. 1972 May;71(1):157–184. doi: 10.1093/genetics/71.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Natzle J. E., Hammonds A. S., Fristrom J. W. Isolation of genes active during hormone-induced morphogenesis in Drosophila imaginal discs. J Biol Chem. 1986 Apr 25;261(12):5575–5583. [PubMed] [Google Scholar]
  13. O'Connor D., Lis J. T. Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation. Nucleic Acids Res. 1981 Oct 10;9(19):5075–5092. doi: 10.1093/nar/9.19.5075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Robertson H. M., Preston C. R., Phillis R. W., Johnson-Schlitz D. M., Benz W. K., Engels W. R. A stable genomic source of P element transposase in Drosophila melanogaster. Genetics. 1988 Mar;118(3):461–470. doi: 10.1093/genetics/118.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Semeshin V. F., Baricheva E. M., Belyaeva E. S., Zhimulev I. F. Electron microscopical analysis of Drosophila polytene chromosomes. III. Mapping of puffs developing from one band. Chromosoma. 1985;91(3-4):234–250. doi: 10.1007/BF00328219. [DOI] [PubMed] [Google Scholar]
  16. Shearn A., Garen A. Genetic control of imaginal disc development in Drosophila. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1393–1397. doi: 10.1073/pnas.71.4.1393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Xiao H., Lis J. T. Heat shock and developmental regulation of the Drosophila melanogaster hsp83 gene. Mol Cell Biol. 1989 Apr;9(4):1746–1753. doi: 10.1128/mcb.9.4.1746. [DOI] [PMC free article] [PubMed] [Google Scholar]

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