Abstract
A DNA sequence capable of inducing the de novo development of fruiting bodies (mushrooms) when integrated into the genome of unmated, nonfruiting strains of the Basidiomycete Schizophyllum commune has been isolated and partially characterized. This sequence, designated FRT1, overrides the normal requirement of a mating interaction for fruiting in this organism. It has been shown to integrate stably in different chromosome locations and appears to be trans-acting. It also enhances the normal process of fruiting that occurs after mating. Additional DNA sequences with similarity to FRT1 were detected within the genome of the strain of origin by hybridization of labeled FRT1 DNA to blots of digested genomic DNAs. FRT1 and the genomic sequences similar to it were shown to be genetically linked. Southern hybridization experiments suggested sequence divergence at the FRT1 locus between different strains of S. commune. A testable model for how FRT1 may act as a key element in the pathway for the differentiation of fruiting bodies is presented as a working hypothesis for further investigation.
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- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Froeliger E. H., Muñoz-Rivas A. M., Specht C. A., Ullrich R. C., Novotny C. P. The isolation of specific genes from the basidiomycete Schizophyllum commune. Curr Genet. 1987;12(7):547–554. doi: 10.1007/BF00419565. [DOI] [PubMed] [Google Scholar]
- Giasson L., Specht C. A., Milgrim C., Novotny C. P., Ullrich R. C. Cloning and comparison of A alpha mating-type alleles of the Basidiomycete Schizophyllum commune. Mol Gen Genet. 1989 Jul;218(1):72–77. doi: 10.1007/BF00330567. [DOI] [PubMed] [Google Scholar]
- Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
- Horton J. S., Raper C. A. Pulsed-field gel electrophoretic analysis of Schizophyllum commune chromosomal DNA. Curr Genet. 1991 Feb;19(2):77–80. doi: 10.1007/BF00326286. [DOI] [PubMed] [Google Scholar]
- Koltin Y., Raper J. R., Simchen G. The genetic structure of the incompatibility factors of Schizophyllum commune: the B factor. Proc Natl Acad Sci U S A. 1967 Jan;57(1):55–62. doi: 10.1073/pnas.57.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leonard T. J., Dick S. CHEMICAL INDUCTION OF HAPLOID FRUITING BODIES IN Schizophyllum commune. Proc Natl Acad Sci U S A. 1968 Mar;59(3):745–751. doi: 10.1073/pnas.59.3.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Munoz-Rivas A., Specht C. A., Drummond B. J., Froeliger E., Novotny C. P., Ullrich R. C. Transformation of the basidiomycete, Schizophyllum commune. Mol Gen Genet. 1986 Oct;205(1):103–106. doi: 10.1007/BF02428038. [DOI] [PubMed] [Google Scholar]
- NIEDERPRUEM D. J. ROLE OF CARBON DIOXIDE IN THE CONTROL OF FRUITING OF SCHIZOPHYLLUM COMMUNE. J Bacteriol. 1963 Jun;85:1300–1308. doi: 10.1128/jb.85.6.1300-1308.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perkins J. H., Gordon S. A. Morphogenesis in Schizophyllum commune. II. Effects of monochromatic light. Plant Physiol. 1969 Dec;44(12):1712–1716. doi: 10.1104/pp.44.12.1712. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raper J. R., Boyd D. H., Raper C. A. Primary and secondary mutations at the incompatibility loci in Schizophyllum. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1324–1332. doi: 10.1073/pnas.53.6.1324. [DOI] [PMC free article] [PubMed] [Google Scholar]