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. 1991 Dec;129(4):1043–1052. doi: 10.1093/genetics/129.4.1043

Doubling Ty1 Element Copy Number in Saccharomyces Cerevisiae: Host Genome Stability and Phenotypic Effects

J D Boeke 1, D J Eichinger 1, G Natsoulis 1
PMCID: PMC1204769  PMID: 1664403

Abstract

Haploid yeast strains bearing approximately double the normal number of Ty1 elements have been constructed using marked GAL/Ty1 fusion plasmids. The strains maintain their high transposon copy number and overall genome structure in the absence of selection. The strains bearing extra Ty1 copies are surprisingly similar phenotypically to the parental strain. The results suggest that the limit to transposon copy number, if any, has not been reached. When these strains are crossed by wild-type strains (i.e., bearing the normal complement of Ty1 elements) or by strains of opposite mating type also bearing excess Ty1 elements, normal to very slightly reduced spore viability is observed, indicating that increasing the extent of transposon homology scattered around the genome does not result in significant increases in frequency of ectopic reciprocal recombination. The results suggest that yeast cells have evolved mechanisms for coping with excess transposon copies in the genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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