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. 1991 Dec;129(4):1119–1136. doi: 10.1093/genetics/129.4.1119

Female Sterile Mutations on the Second Chromosome of Drosophila Melanogaster. II. Mutations Blocking Oogenesis or Altering Egg Morphology

T Schupbach 1, E Wieschaus 1
PMCID: PMC1204776  PMID: 1783295

Abstract

In mutagenesis screens for recessive female sterile mutations on the second chromosome of Drosophila melanogaster 528 lines were isolated which allow the homozygous females to survive but cause sterility. In 62 of these lines early stages of oogenesis are affected, and these females usually do not lay any eggs. In 333 lines oogenesis proceeds apparently normally to stage 8 of oogenesis, but morphological defects become often apparent during later stages of oogenesis, and are visible in the defective eggs produced by these females whereas 133 lay eggs that appear morphologically normal, but do not support normal embryonic development. Of the lines 341 have been genetically characterized and define a total of 140 loci on the second chromosome. Not all the loci are specific for oogenesis. From the numbers obtained we estimate that the second chromosome of Drosophila contains about 13 loci that are relatively specific for early oogenesis, 70 loci that are specifically required in mid to late oogenesis, and around 30 maternal-effect lethals.

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Selected References

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  1. Ashburner M., Thompson P., Roote J., Lasko P. F., Grau Y., el Messal M., Roth S., Simpson P. The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. VII. Characterization of the region around the snail and cactus loci. Genetics. 1990 Nov;126(3):679–694. doi: 10.1093/genetics/126.3.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gans M., Audit C., Masson M. Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster. Genetics. 1975 Dec;81(4):683–704. doi: 10.1093/genetics/81.4.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Garcia-Bellido A., Robbins L. G. Viability of Female Germ-Line Cells Homozygous for Zygotic Lethals in DROSOPHILA MELANOGASTER. Genetics. 1983 Feb;103(2):235–247. doi: 10.1093/genetics/103.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grossniklaus U., Bellen H. J., Wilson C., Gehring W. J. P-element-mediated enhancer detection applied to the study of oogenesis in Drosophila. Development. 1989 Oct;107(2):189–200. doi: 10.1242/dev.107.2.189. [DOI] [PubMed] [Google Scholar]
  5. Hay B., Jan L. Y., Jan Y. N. A protein component of Drosophila polar granules is encoded by vasa and has extensive sequence similarity to ATP-dependent helicases. Cell. 1988 Nov 18;55(4):577–587. doi: 10.1016/0092-8674(88)90216-4. [DOI] [PubMed] [Google Scholar]
  6. Klingler M., Erdélyi M., Szabad J., Nüsslein-Volhard C. Function of torso in determining the terminal anlagen of the Drosophila embryo. Nature. 1988 Sep 15;335(6187):275–277. doi: 10.1038/335275a0. [DOI] [PubMed] [Google Scholar]
  7. Komitopoulou K., Gans M., Margaritis L. H., Kafatos F. C., Masson M. Isolation and Characterization of Sex-Linked Female-Sterile Mutants in DROSOPHILA MELANOGASTER with Special Attention to Eggshell Mutants. Genetics. 1983 Dec;105(4):897–920. doi: 10.1093/genetics/105.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lasko P. F., Ashburner M. Posterior localization of vasa protein correlates with, but is not sufficient for, pole cell development. Genes Dev. 1990 Jun;4(6):905–921. doi: 10.1101/gad.4.6.905. [DOI] [PubMed] [Google Scholar]
  9. Lasko P. F., Ashburner M. The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A. Nature. 1988 Oct 13;335(6191):611–617. doi: 10.1038/335611a0. [DOI] [PubMed] [Google Scholar]
  10. Lehmann R., Nüsslein-Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the Drosophila embryo. Development. 1991 Jul;112(3):679–691. doi: 10.1242/dev.112.3.679. [DOI] [PubMed] [Google Scholar]
  11. Manseau L. J., Schüpbach T. cappuccino and spire: two unique maternal-effect loci required for both the anteroposterior and dorsoventral patterns of the Drosophila embryo. Genes Dev. 1989 Sep;3(9):1437–1452. doi: 10.1101/gad.3.9.1437. [DOI] [PubMed] [Google Scholar]
  12. Margaritis L. H., Kafatos F. C., Petri W. H. The eggshell of Drosophila melanogaster. I. Fine structure of the layers and regions of the wild-type eggshell. J Cell Sci. 1980 Jun;43:1–35. doi: 10.1242/jcs.43.1.1. [DOI] [PubMed] [Google Scholar]
  13. McKearin D. M., Spradling A. C. bag-of-marbles: a Drosophila gene required to initiate both male and female gametogenesis. Genes Dev. 1990 Dec;4(12B):2242–2251. doi: 10.1101/gad.4.12b.2242. [DOI] [PubMed] [Google Scholar]
  14. Mohler J. D. Developmental genetics of the Drosophila egg. I. Identification of 59 sex-linked cistrons with maternal effects on embryonic development. Genetics. 1977 Feb;85(2):259–272. doi: 10.1093/genetics/85.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mohler J., Wieschaus E. F. Dominant maternal-effect mutations of Drosophila melanogaster causing the production of double-abdomen embryos. Genetics. 1986 Apr;112(4):803–822. doi: 10.1093/genetics/112.4.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Orr W. C., Galanopoulos V. K., Romano C. P., Kafatos F. C. A female sterile screen of the Drosophila melanogaster X chromosome using hybrid dysgenesis: identification and characterization of egg morphology mutants. Genetics. 1989 Aug;122(4):847–858. doi: 10.1093/genetics/122.4.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perrimon N., Engstrom L., Mahowald A. P. The effects of zygotic lethal mutations on female germ-line functions in Drosophila. Dev Biol. 1984 Oct;105(2):404–414. doi: 10.1016/0012-1606(84)90297-5. [DOI] [PubMed] [Google Scholar]
  18. Perrimon N., Mohler D., Engstrom L., Mahowald A. P. X-linked female-sterile loci in Drosophila melanogaster. Genetics. 1986 Jul;113(3):695–712. doi: 10.1093/genetics/113.3.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reuter G., Szidonya J. Cytogenetic analysis of variegation suppressors and a dominant temperature-sensitive lethal in region 23-26 of chromosome 2L in Drosophila melanogaster. Chromosoma. 1983;88(4):277–285. doi: 10.1007/BF00292904. [DOI] [PubMed] [Google Scholar]
  20. Romano C. P., Bienz-Tadmor B., Mariani B. D., Kafatos F. C. Both early and late Drosophila chorion gene promoters confer correct temporal, tissue and sex specificity on a reporter Adh gene. EMBO J. 1988 Mar;7(3):783–790. doi: 10.1002/j.1460-2075.1988.tb02876.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schüpbach T. Germ line and soma cooperate during oogenesis to establish the dorsoventral pattern of egg shell and embryo in Drosophila melanogaster. Cell. 1987 Jun 5;49(5):699–707. doi: 10.1016/0092-8674(87)90546-0. [DOI] [PubMed] [Google Scholar]
  22. Schüpbach T. Normal female germ cell differentiation requires the female X chromosome to autosome ratio and expression of sex-lethal in Drosophila melanogaster. Genetics. 1985 Mar;109(3):529–548. doi: 10.1093/genetics/109.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Steward R., Nüsslein-Volhard C. The genetics of the dorsal-Bicaudal-D region of Drosophila melanogaster. Genetics. 1986 Jul;113(3):665–678. doi: 10.1093/genetics/113.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Strecker T. R., Halsell S. R., Fisher W. W., Lipshitz H. D. Reciprocal effects of hyper- and hypoactivity mutations in the Drosophila pattern gene torso. Science. 1989 Feb 24;243(4894 Pt 1):1062–1066. doi: 10.1126/science.2922596. [DOI] [PubMed] [Google Scholar]
  25. Suter B., Romberg L. M., Steward R. Bicaudal-D, a Drosophila gene involved in developmental asymmetry: localized transcript accumulation in ovaries and sequence similarity to myosin heavy chain tail domains. Genes Dev. 1989 Dec;3(12A):1957–1968. doi: 10.1101/gad.3.12a.1957. [DOI] [PubMed] [Google Scholar]
  26. Underwood E. M., Briot A. S., Doll K. Z., Ludwiczak R. L., Otteson D. C., Tower J., Vessey K. B., Yu K. Genetics of 51D-52A, a region containing several maternal-effect genes and two maternal-specific transcripts in Drosophila. Genetics. 1990 Nov;126(3):639–650. doi: 10.1093/genetics/126.3.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wharton R. P., Struhl G. Structure of the Drosophila BicaudalD protein and its role in localizing the the posterior determinant nanos. Cell. 1989 Dec 1;59(5):881–892. doi: 10.1016/0092-8674(89)90611-9. [DOI] [PubMed] [Google Scholar]
  28. Wieschaus E., Audit C., Masson M. A clonal analysis of the roles of somatic cells and germ line during oogenesis in Drosophila. Dev Biol. 1981 Nov;88(1):92–103. doi: 10.1016/0012-1606(81)90221-9. [DOI] [PubMed] [Google Scholar]
  29. Wieschaus E., Szabad J. The development and function of the female germ line in Drosophila melanogaster: a cell lineage study. Dev Biol. 1979 Jan;68(1):29–46. doi: 10.1016/0012-1606(79)90241-0. [DOI] [PubMed] [Google Scholar]
  30. Wustmann G., Szidonya J., Taubert H., Reuter G. The genetics of position-effect variegation modifying loci in Drosophila melanogaster. Mol Gen Genet. 1989 Jun;217(2-3):520–527. doi: 10.1007/BF02464926. [DOI] [PubMed] [Google Scholar]

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