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. 1992 Jun;131(2):449–459. doi: 10.1093/genetics/131.2.449

Germinal and Somatic Activity of the Maize Element Activator (Ac) in Arabidopsis

J Keller 1, E Lim 1, D W James-Jr 1, H K Dooner 1
PMCID: PMC1205017  PMID: 1322854

Abstract

We have investigated the germinal and somatic activity of the maize Activator (Ac) element in Arabidopsis with the objective of developing an efficient transposon-based system for gene isolation in that plant. Transposition activity was assayed with a chimeric marker that consists of the cauliflower mosaic virus 35S promoter and a bacterial streptomycin phosphotransferase gene (SPT). Somatic activity was detected in seedlings germinated on plates containing streptomycin as green-resistant sectors against a background of white-sensitive cells. Germinal excisions resulted in fully green seedlings. The transposition frequency was extremely low when a single copy of the transposon was present, but appeared to increase with an increase in Ac copy number. Plants that were selected as variegated produced an increased number of green progeny. The methylation state of the Ac elements in lines with either low or high levels of excision was assessed by restriction analysis. No difference was found between these lines, indicating that the degree of methylation did not contribute to the level of Ac activity. Germinal excision events were analyzed molecularly and shown to carry reinserted transposons in about 50% of the cases. In several instances, streptomycin-resistant siblings carried the same transposed Ac element, indicating that excision had occurred prior to meiosis in the parent. We discuss parameters that need to be considered to optimize the use of Ac as a transposon tag in Arabidopsis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carpenter R., Coen E. S. Floral homeotic mutations produced by transposon-mutagenesis in Antirrhinum majus. Genes Dev. 1990 Sep;4(9):1483–1493. doi: 10.1101/gad.4.9.1483. [DOI] [PubMed] [Google Scholar]
  2. Chomet P. S., Wessler S., Dellaporta S. L. Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J. 1987 Feb;6(2):295–302. doi: 10.1002/j.1460-2075.1987.tb04753.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cone K. C., Schmidt R. J., Burr B., Burr F. A. Advantages and limitations of using Spm as a transposon tag. Basic Life Sci. 1988;47:149–159. doi: 10.1007/978-1-4684-5550-2_11. [DOI] [PubMed] [Google Scholar]
  4. Dooner H. K., Belachew A. Transposition Pattern of the Maize Element Ac from the Bz-M2(ac) Allele. Genetics. 1989 Jun;122(2):447–457. doi: 10.1093/genetics/122.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Greenblatt I. M. A chromosome replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element, modulator, in maize. Genetics. 1984 Oct;108(2):471–485. doi: 10.1093/genetics/108.2.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Haring M. A., Rommens C. M., Nijkamp H. J., Hille J. The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies. Plant Mol Biol. 1991 Mar;16(3):449–461. doi: 10.1007/BF00023995. [DOI] [PubMed] [Google Scholar]
  7. Hehl R., Baker B. Properties of the maize transposable element Activator in transgenic tobacco plants: a versatile inter-species genetic tool. Plant Cell. 1990 Aug;2(8):709–721. doi: 10.1105/tpc.2.8.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones J. D., Carland F. M., Maliga P., Dooner H. K. Visual detection of transposition of the maize element activator (ac) in tobacco seedlings. Science. 1989 Apr 14;244(4901):204–207. doi: 10.1126/science.244.4901.204. [DOI] [PubMed] [Google Scholar]
  9. Jones J. D., Carland F., Lim E., Ralston E., Dooner H. K. Preferential transposition of the maize element Activator to linked chromosomal locations in tobacco. Plant Cell. 1990 Aug;2(8):701–707. doi: 10.1105/tpc.2.8.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keith B., Chua N. H. Monocot and dicot pre-mRNAs are processed with different efficiencies in transgenic tobacco. EMBO J. 1986 Oct;5(10):2419–2425. doi: 10.1002/j.1460-2075.1986.tb04516.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koncz C., Mayerhofer R., Koncz-Kalman Z., Nawrath C., Reiss B., Redei G. P., Schell J. Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana. EMBO J. 1990 May;9(5):1337–1346. doi: 10.1002/j.1460-2075.1990.tb08248.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marks M. D., Feldmann K. A. Trichome Development in Arabidopsis thaliana. I. T-DNA Tagging of the GLABROUS1 Gene. Plant Cell. 1989 Nov;1(11):1043–1050. doi: 10.1105/tpc.1.11.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCLINTOCK B. Chromosome organization and genic expression. Cold Spring Harb Symp Quant Biol. 1951;16:13–47. doi: 10.1101/sqb.1951.016.01.004. [DOI] [PubMed] [Google Scholar]
  14. Van Sluys M. A., Tempé J., Fedoroff N. Studies on the introduction and mobility of the maize Activator element in Arabidopsis thaliana and Daucus carota. EMBO J. 1987 Dec 20;6(13):3881–3889. doi: 10.1002/j.1460-2075.1987.tb02728.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yanofsky M. F., Ma H., Bowman J. L., Drews G. N., Feldmann K. A., Meyerowitz E. M. The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature. 1990 Jul 5;346(6279):35–39. doi: 10.1038/346035a0. [DOI] [PubMed] [Google Scholar]

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