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. 1992 Aug;131(4):811–819. doi: 10.1093/genetics/131.4.811

Strand-Specificity in the Transformation of Yeast with Synthetic Oligonucleotides

T Yamamoto 1, R P Moerschell 1, L P Wakem 1, S Komar-Panicucci 1, F Sherman 1
PMCID: PMC1205094  PMID: 1325385

Abstract

Cyc1 mutants of the yeast Saccharomyces cerevisiae were directly transformed with both sense and antisense oligonucleotides to examine the involvement of the two genomic DNA strands in transformation. Sense oligonucleotides yielded approximately 20-fold more transformants than antisense oligonucleotides. This differential effect was observed with oligonucleotides designed to make alterations at six different sites along the gene and was independent of the oligonucleotide sequence and length, number of mismatches and the host strain. Competition studies showed that antisense oligonucleotides did not inhibit transformation. Although the mechanism for this strand specificity is unknown, this difference was maintained even when CYC1 transcription was diminished to approximately 2% of the normal level.

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Selected References

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