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. 1992 Aug;131(4):917–927. doi: 10.1093/genetics/131.4.917

A P Element Chimera Containing Captured Genomic Sequences Was Recovered at the Vestigial Locus in Drosophila following Targeted Transposition

T R Heslip 1, J A Williams 1, J B Bell 1, R B Hodgetts 1
PMCID: PMC1205102  PMID: 1325388

Abstract

A P element carrying the Dopa decarboxylase gene, P[Ddc], was targeted into vg(21), a cryptic P element induced mutant allele of the vestigial (vg) locus. The resulting allele, vg(28w), contained the expected P[Ddc] plus an additional 9.5 kb of DNA, captured from elsewhere on chromosome II. Reversion of the vg(28w) mutant allele demonstrated that the entire insert can excise but cannot reinsert at an appreciable frequency. We explain the targeted transposition as the repair of a double stranded gap, created by the excision of the P element at vg(21), and suggest that the formation of chimeric elements may be an important component of P element dependent genomic instability.

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Selected References

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  1. Ballinger D. G., Benzer S. Targeted gene mutations in Drosophila. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9402–9406. doi: 10.1073/pnas.86.23.9402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg C. A., Spradling A. C. Studies on the rate and site-specificity of P element transposition. Genetics. 1991 Mar;127(3):515–524. doi: 10.1093/genetics/127.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bingham P. M., Kidwell M. G., Rubin G. M. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982 Jul;29(3):995–1004. doi: 10.1016/0092-8674(82)90463-9. [DOI] [PubMed] [Google Scholar]
  4. Cooley L., Thompson D., Spradling A. C. Constructing deletions with defined endpoints in Drosophila. Proc Natl Acad Sci U S A. 1990 Apr;87(8):3170–3173. doi: 10.1073/pnas.87.8.3170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Engels W. R. A trans-acting product needed for P factor transposition in Drosophila. Science. 1984 Dec 7;226(4679):1194–1196. doi: 10.1126/science.6095450. [DOI] [PubMed] [Google Scholar]
  6. Engels W. R., Johnson-Schlitz D. M., Eggleston W. B., Sved J. High-frequency P element loss in Drosophila is homolog dependent. Cell. 1990 Aug 10;62(3):515–525. doi: 10.1016/0092-8674(90)90016-8. [DOI] [PubMed] [Google Scholar]
  7. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  8. Green M. M., Shepherd S. H. Genetic instability in Drosophila melanogaster: the induction of specific chromosome 2 deletions by MR elements. Genetics. 1979 Jul;92(3):823–832. doi: 10.1093/genetics/92.3.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hawley R. S., Steuber R. A., Marcus C. H., Sohn R., Baronas D. M., Cameron M. L., Zitron A. E., Chase J. W. Molecular analysis of an unstable P element insertion at the singed locus of Drosophila melanogaster: evidence for intracistronic transposition of a P element. Genetics. 1988 May;119(1):85–94. doi: 10.1093/genetics/119.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ish-Horowicz D., Pinchin S. M., Schedl P., Artavanis-Tsakonas S., Mirault M. E. Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci of D. melanogaster. Cell. 1979 Dec;18(4):1351–1358. doi: 10.1016/0092-8674(79)90245-9. [DOI] [PubMed] [Google Scholar]
  11. Karess R. E., Rubin G. M. Analysis of P transposable element functions in Drosophila. Cell. 1984 Aug;38(1):135–146. doi: 10.1016/0092-8674(84)90534-8. [DOI] [PubMed] [Google Scholar]
  12. Kidwell M. G., Kidwell J. F., Sved J. A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977 Aug;86(4):813–833. doi: 10.1093/genetics/86.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mullins M. C., Rio D. C., Rubin G. M. cis-acting DNA sequence requirements for P-element transposition. Genes Dev. 1989 May;3(5):729–738. doi: 10.1101/gad.3.5.729. [DOI] [PubMed] [Google Scholar]
  14. O'Hare K., Rubin G. M. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. doi: 10.1016/0092-8674(83)90133-2. [DOI] [PubMed] [Google Scholar]
  15. O'Kane C. J., Gehring W. J. Detection in situ of genomic regulatory elements in Drosophila. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9123–9127. doi: 10.1073/pnas.84.24.9123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rio D. C. Regulation of Drosophila P element transposition. Trends Genet. 1991 Sep;7(9):282–287. doi: 10.1016/0168-9525(91)90309-E. [DOI] [PubMed] [Google Scholar]
  17. Robertson H. M., Preston C. R., Phillis R. W., Johnson-Schlitz D. M., Benz W. K., Engels W. R. A stable genomic source of P element transposase in Drosophila melanogaster. Genetics. 1988 Mar;118(3):461–470. doi: 10.1093/genetics/118.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rubin G. M., Kidwell M. G., Bingham P. M. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982 Jul;29(3):987–994. doi: 10.1016/0092-8674(82)90462-7. [DOI] [PubMed] [Google Scholar]
  19. Scholnick S. B., Morgan B. A., Hirsh J. The cloned dopa decarboxylase gene is developmentally regulated when reintegrated into the Drosophila genome. Cell. 1983 Aug;34(1):37–45. doi: 10.1016/0092-8674(83)90134-4. [DOI] [PubMed] [Google Scholar]
  20. Searles L. L., Jokerst R. S., Bingham P. M., Voelker R. A., Greenleaf A. L. Molecular cloning of sequences from a Drosophila RNA polymerase II locus by P element transposon tagging. Cell. 1982 Dec;31(3 Pt 2):585–592. doi: 10.1016/0092-8674(82)90314-2. [DOI] [PubMed] [Google Scholar]
  21. Spradling A. C., Rubin G. M. The effect of chromosomal position on the expression of the Drosophila xanthine dehydrogenase gene. Cell. 1983 Aug;34(1):47–57. doi: 10.1016/0092-8674(83)90135-6. [DOI] [PubMed] [Google Scholar]
  22. Tsubota S. I., Huong D. V. Capture of flanking DNA by a P element in Drosophila melanogaster: creation of a transposable element. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):693–697. doi: 10.1073/pnas.88.3.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Williams J. A., Atkin A. L., Bell J. B. The functional organization of the vestigial locus in Drosophila melanogaster. Mol Gen Genet. 1990 Mar;221(1):8–16. doi: 10.1007/BF00280361. [DOI] [PubMed] [Google Scholar]
  24. Williams J. A., Bell J. B., Carroll S. B. Control of Drosophila wing and haltere development by the nuclear vestigial gene product. Genes Dev. 1991 Dec;5(12B):2481–2495. doi: 10.1101/gad.5.12b.2481. [DOI] [PubMed] [Google Scholar]
  25. Williams J. A., Pappu S. S., Bell J. B. Molecular analysis of hybrid dysgenesis-induced derivatives of a P-element allele at the vg locus. Mol Cell Biol. 1988 Apr;8(4):1489–1497. doi: 10.1128/mcb.8.4.1489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wright T. R., Beermann W., Marsh J. L., Bishop C. P., Steward R., Black B. C., Tomsett A. D., Wright E. Y. The genetics of dopa decarboxylase in Drosophila melanogaster. IV. The genetics and cytology of the 37B10-37D1 region. Chromosoma. 1981;83(1):45–58. doi: 10.1007/BF00286015. [DOI] [PubMed] [Google Scholar]

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