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. 1992 Dec;132(4):943–950. doi: 10.1093/genetics/132.4.943

Spontaneous Amplification of the Adh4 Gene in Saccharomyces Cerevisiae

M Dorsey 1, C Peterson 1, K Bray 1, C E Paquin 1
PMCID: PMC1205250  PMID: 1459445

Abstract

Five spontaneous amplifications of the ADH4 gene were identified among 1,894 antimycin A-resistant mutants isolated from a diploid strain after growth at 15°. Four of these amplifications are ~40-kb linear extrachromosomal palindromes carrying telomere homologous sequences at each end similar to a previously isolated amplification. ADH4 is located at the extreme left end of chromosome VII, and the extrachromosomal fragments appear to be the fusion of two copies of the end of this chromosome. The fifth amplification is a chromosomal amplification carrying an extra copy of ADH4 on both homologs of chromosome VII. These results suggest that the ADH system can be used to study amplification in Saccharomyces cerevisiae.

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Selected References

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